Cell adhesion plasma polymerization

Hatakeyama H, Kikuchi A, Yamato M et al (2007) Patterned biofunctional designs of thermo-responsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally induced detachment. Biomaterials 28 3632-3643 Hem DL, Hubbell JA (1998) Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing. J Biomed Mater Res 39 266-276 Huang J, Wang XL, Chen XZ et al (2003) Temperature-sensitive membranes prepared by the plasma-induced graft polymerization of N-isopropylacrylamide into porous polyethylene membranes. J Appl Polym Sci 89 3180-3187... 

Anticoagulants, e.g., heparin and hirudin, antimicrobials, cell adhesion peptides, cell adhesion proteins, negative surface charge plasma polymerized coating, thrombolytics Tissue Adhesives... 

The necessary functional groups to activate the surface with the spacer molecule benzoquinone on the Mitrathane surface [141] were introduced using plasma polymerization of vinyl acetate followed by saponification. Besides the immobilization of single amino acids, the fragment of fibronectin GRGDS as cell adhesion mediator was immobilized [142,143]. 

To achieve enhanced cell adhesion and proliferation in vitro which probably leads to a better integration of the prosthesis into the surrounding tissue the cell adhesion mediator fibronectin was coupled to the surface of silicone. Figure 40 shows the two principal steps of the appHed surface modification procedure. Argon plasma treatment and subsequent exposure to air of the silicone samples leads to the formation of hydroperoxide groups on its surface which are used to initiate polymerization of acrylic acid (AAc), methacrylic acid (MAAc) and gly-cidyl methacrylate (GMA) generating carboxylic and epoxy groups, respectively. 

Kim (2002) Plasma Polymerization of acrylamide Chinese hamster ovary cells adhesion [44]... 

As an extension to this surface-modification method, researchers have utilized plasma polymerization of acrylic acid to immobilize biologically active molecules, such as recombinant human bone formation protein-2 (rhBMP-2). rhBMP-2 is a signaling molecule that promotes bone formation by osteoinduction that has been utilized for various orthopedic tissue-engineering applications (Kim et al., 2013). One research group modified a PCL scaffold surface with plasma-polymerized acrylic acid (PPAA) and rhBMP-2 via electrostatic interactions (Kim et al., 2013) (which is outside of the scope of this chapter). This interesting approach may be apphed to the surface modification of solid fillers and provide additional benefits compared to the surface-modification techniques currently utihzed in orthopedic polymeric biocomposite development. The acrylic acid and rhBMP-2-modifled surface showed improved cell attachment and adhesion compared to the surface with acrylic acid alone. The ability to modify the surface of a solid-filler particle in a polymeric biocomposite with a bioactive molecule, such as rhBMP-2, provides a delivery vehicle for the bioactive molecule to the polymeric biocomposite and the eventual implantation site of this biomaterial. Such surface-modification and immobihzation approaches may provide a method to control the release kinetics of attached molecules to the localized bone-defect site. 

R.A. Pareta, A.B. Reising, T. Miller, D. Storey, T.J. Webster, Increased endothelial cell adhesion on plasma modified nanostructured polymeric and metallic surfaces for vascular stent applications, Biotechnol. Bioeng. 103 (2009) 459-471. 

Surface modification with hydrophilic polymers, such as poly(ethylene oxide) (PEO), has been beneficial in improving the blo( compatibility of polymeric biomaterials. Surface-bound PEO is expected to prevent plasma protein adsoiption, platelet adhesion, and bacterial adhesion by the steric repulsion mechanism. PEO-rich surfaces have been prepared either by physical adsorption, or by covalent grafting to the surface. Physically adsorbed PEO homopolymers and copolymers are not very effective since they can be easily displaced from the surface by plasma proteins and cells. Covalent grafting, on the other hand, provides a permanent layer of PEO on the surface. Various methods of PEO grafting to the surface and their effect on plasma protein adsorption, platelet adhesion, and bacterial adhesion is discussed. 

Lopez-Perez, P.M., Marques, A.P., da SUva, R.M.P., Pashkuleva, I. and Reis, R.L. 2007. Effect of chitosan membrane surface modification via plasma induced polymerization on the adhesion of osteoblast-like cells. 

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