Adhesive peptides

Hem DL, Hubbell JA (1998) Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing. J Biomed Mater Res 39 266-276... 

Feng Y, Mrksich M (2004) The synergy peptide PHSRN and the adhesion peptide RGD mediate cell adhesion through a common mechanism. Biochemistry 43 15811-15821... 

IL-8 upregulated via integrin engagement of RGD adhesion peptides in a hydrogel matrix... 

Synthesis of Biodegradable Poiy(lactic acid-co-lysine) Copolymers Linked to Cell Adhesive Peptide Sequences General Procedure 27 ... 

Kato R, Kaga C, Kunimatsu M et al (2006) Peptide array-based interaction assay of solid-bound peptides and anchorage-dependant cells and its effectiveness in cell-adhesive peptide design. J Biosci Bioeng 101(6) 485—495... 

Fig. 9 Reversible cell adhesion by photochemical control of azobenzene SAMs on gold. Interconversion of Z and E configurations (top). Cell adhesive peptide, RGD, is displayed when SAMs adopt the E configuration at 450-490 nm, and cells adhere and grow (bottom left and right). At 340-380 nm, azobenzene converts to the Z conformation, masking RGD, and cell adhesion is prevented (center bottom). Reproduced from [148] with permission. Copyright Wiley, 2009...

Fig. 2. Schematic representation (not to scale) of the amino acid sequence of a Type I collagen. A Type I collagen consists of two identical of cd(I) and one unique a2(I) chains whose combination produces a triple helical structure 300 nm long and 0.5 nm thick, with a periodicity of 67 nm. Arginine-glycine-aspartic acid adhesive peptides ( ) start at peptide sequence 1083 in the cd(I) chain as well as at 772, 822, and 1005 in the a2(l) chain. (The schematic was redrawn using information obtained from the following references Ayad et al, 1994 Mathews and van Holde, 1990 and Darnell et al, 1990.)...

Brandley, B. K. and Schnaar, R. L. (1989). Tumor cell haptotaxis on covalently immobilized linear and exponential gradients of a cell adhesion peptide. Dev. Biol. 135, 74-86. 

Harfenist EJ, Packham MA, Mustard IF. Effects of the cdl adhesion peptide, Arg-Gly-Asp-Ser, cm responses ofwashedplatdets from humans, rabbits and rats Blood 1988 71 132-136... 

One of the most prevalent cell adhesion peptides is the tripeptide arginine-glycine-aspartate (RGD) from fibronectin.f This tripeptide has been used extensively to promote adhesion and spreading of many cell types. For example, polyurethane surface can be activated and coupled to RGD via the carboxy terminus. Surfaces containing photo-activable groups, such as benzophenone or aryl azide, can be modified easily with RGD by UV radiation. This tripeptide and other... 

Besides solid material surfaces, functional water-soluble polymers have also been created to promote cell-cell adhesion in aqueous media. For example, cell adhesion peptides of RGD or tyrosine-isoleucine-glycine-serine-arginine (YIGSR) have been attached to the ends of biofunctional PEG and shown to induce neural cell aggregation in culture media. ° ... 

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