Capillary electrochromatography pressure-assisted

Ivanov, A. R., Horvath, C. and Karger, B. L. High-efficiency peptide analysis on monolithic multi-mode capillary columns Pressure-assisted capillary electrochromatography/capillary electrophoresis coupled to UV and electrospray ionization-mass spectrometry. Electrophoresis, 24,3663, 2003. [Pg.287]

Pressurization of the vials at both the inlet and the outlet ends of the CEC capillary column packed with particles to about 1.2 MPa is required to prevent formation of bubbles that lead to a noisy baseline. Typically, equal pressure of an inert gas such as nitrogen is applied to both vials to avoid flow that would otherwise occur resulting from the pressure difference. Hydraulic pressure applied only at the inlet end of the capillary column is occasionally used in pressure-assisted electrochromatography [38,39]. [Pg.12]

Two successful approaches of pressurization are known (i) Both the inlet and the outlet buffer vial are constantly pressurized [10-13]. (ii) Pressure can be applied either to the inlet [9,14-18] or the outlet end of the capillary [5,6], This technique is called pressure supported or pressure assisted electrochromatography (other synonyms are pressurized CEC, pressurized flow CEC), pressure electrochromatography (PEC), pseudoelectrochromatography (pEC) or electro-HPLC [9,14,15,19-21]). [Pg.332]

Capillary electrochromatography (CEC) — A special case of capillary liquid chromatography, in which the mobile phase motion is driven by -> electroosmotic volume flow through a capillary, filled, packed, or coated with a stationary phase, (which may be assisted by pressure). The retention time is determined by a combination of -> electrophoretic mobility and chromatographic retention. [Pg.70]

Higher pressures are required for pressure-assisted separations in packed columns used for capillary electrochromatography [235,376,464-466]. These columns require a high-pressure pump to provide the supplementary mobile phase flow. Conventional rotary injection valves and autosamplers can be used for sample introduction with this arrangement if a special inlet tee housing the electrode and split line with a restrictor is installed [422]. Microcolumn pumps are also useful for conditioning columns before initial use in capillary electrochromatography (section 8.4.2). There are no com-... [Pg.694]

Lu, M. Zhang, L. Feng, Q. Xia, S. Chi, Y. Tong, P. Chen, G. Pressure-assisted capillary electrochromatography with electrospray ionization-mass spectrometry based on silica-based monolithic column for rapid analysis of narcotics. Electrophoresis 2008, 29, 936-943. [Pg.202]

DGE a AC AMS APCI API AP-MALDI APPI ASAP BIRD c CAD CE CF CF-FAB Cl CID cw CZE Da DAPCI DART DC DE DESI DIOS DTIMS EC ECD El ELDI EM ESI ETD eV f FAB FAIMS FD FI FT FTICR two-dimensional gel electrophoresis atto, 10 18 alternating current accelerator mass spectrometry atmospheric pressure chemical ionization atmospheric pressure ionization atmospheric pressure matrix-assisted laser desorption/ionization atmospheric pressure photoionization atmospheric-pressure solids analysis probe blackbody infrared radiative dissociation centi, 10-2 collision-activated dissociation capillary electrophoresis continuous flow continuous flow fast atom bombardment chemical ionization collision-induced dissociation continuous wave capillary zone electrophoresis dalton desorption atmospheric pressure chemical ionization direct analysis in real time direct current delayed extraction desorption electrospray ionization desorption/ionization on silicon drift tube ion mobility spectrometry electrochromatography electron capture dissociation electron ionization electrospray-assisted laser desorption/ionization electron multiplier electrospray ionization electron transfer dissociation electron volt femto, 1CT15 fast atom bombardment field asymmetric waveform ion mobility spectrometry field desorption field ionization Fourier transform Fourier transform ion cyclotron resonance... [Pg.11]