Packed capillary column

Packed capillary (PC) columns were originally developed by Novotny and coworkers and may be regarded as a hybrid between packed 

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Slurry packing techniques are required for the preparation of efficient columns with rigid particles of less than 20 micrometers in diameter. The same general packing apparatus. Figure 4.8, can be used to pack columns by the balanced-density slurry, liquid slurry, or the viscous slurry techniques. Down-fill slurry packing is the method of choice for small bore columns and packed capillary columns. 

COMPARATIVE COLUMN PERFORMANCE DATA FOR SCH(B MICROPACKED AMD PACKED CAPILLARY COLUMNS... 

One of the major practical problems to the installation of HPLC as a permanent process monitor is the need to replace solvent. A large solvent reservoir may present problems both in terms of size and safety. One solution is the use of packed capillary columns, which consume much less solvent than conventional columns, as the comparison (at constant linear velocity) in Table 1 shows. 

Trisciani, A. and Andreolini, F, Evaluation of a micro-HPLC system dedicated to packed capillary column liquid chromatography, /. HRC CC, 13,270,1990. 

Applications High-temperature liquid chromatography with packed-capillary columns, nonaqueous mobile phases, and ELSD and ICP-MS detection, has been developed specifically as a robust analytical tool for the analysis of high-MW polymer additives [731,738]. Dissolving such moderately polar, heavy compounds with low water solubility at ambient temperature usually... 

Miniaturised SEC uses small fused-silica packed-capillary columns (0.32-1 mm i.d., 30-200cm) instead of relatively large metal columns. Miniaturisation puts stringent requirements on the quality of SEC columns. Advantages of ptSEC are (i) much smaller amounts of (toxic, expensive) solvents (ii) smaller samples (iii) better and easier temperature control (iv) increased detector compatibility (e.g. MS) and (v) greatly reduced... 

SFE can be combined with several forms of SFC, i.e. with conventional packed columns (l-4.6mm i.d. packed-column SFC or pSFC), with capillary columns (10-250 xm i.d. capillary SFC or cSFC), and recently with packed capillary columns (200-530 p,m i.d., 3-10 xm particles packed capillary SFC or pc-SFC). 

MacNair, J.E., Lewis, K.C., Jorgenson, J.W. (1997). Ultrahigh-pressure reversed-phase liquid chromatography in packed capillary columns. Anal. Chem. 69, 983-989. 

Fig. 30. Effect of field strength and percentage of acetonitrile in the mobile phase on electroosmotic flow in a packed capillary column. (Reprinted with permission from [35]. Copyright 2000 Elsevier). Conditions capillary column 100 pm i.d., total length 38 cm, active length 8.5 cm packed with 0.5-pm C8 silica beads, mobile phase acetonitrile/25 mmol/1 TRIS-HCl buffer pH = 8, temperature 20 °C, marker thiourea...

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