Peptide, analysis

Modern methods of amino-acid and peptide analysis, have enabled the complete amino-acid sequence of a number of proteins to be worked out. The grosser structure can be determined by X-ray diffraction procedures. Proteins have molecular weights ranging from about 6 000 000 to 5 000 (although the dividing line between a protein and a peptide is ill defined). Edible proteins can be produced from petroleum and nutrients under fermentation. 

E. Gross and J. Meinenhofer, Eds., The Peptides Analysis. Synthesis, Biology Vol. 3 Protection of Functional Groups in Peptide Synthesis, Academic Press, New York, 1981. 

Table 5.5 Nomenclature of the ions formed in the mass spectral fragmentation of polypeptides. From Chapman, J. R. (Ed.), Protein and Peptide Analysis by Mass Spectrometry, Methods in Molecular Biology, Vol. 61, 1996. Reproduced by permission of Humana Press, Inc. 

J. R. (Ed.), Protein and Peptide Analysis by Mass Spectrometry, Methods in Molecular Biology, Vol. 61, 1996. 

DEAE 25W were operated in tandem to map the peptides generated on tryptic hydrolysis of very large proteins, such as albumin genetic variants.197 A useful review that covers many aspects of peptide analysis is available.198... 

Hartley, R. W., Peptide analysis and preparation with an amino acid analyzer, Anal. Biochem., 46, 676, 1972. 

The pH used in the first C18 separation dimension was rather high (pH 10), however, no peptide loses or carryover, due to on-column precipitation, were observed. Peak shape was comparable to peptide analysis at low pH. Modern stationary phases, based on hybrid silica and stable alkyl bonding chemistry, are well suited for chromatography at extreme pH without compromising column lifetime or analysis-to-analysis reproducibility (Wyndham et al., 2003). 

Janini, G.M., Chan, K.C., Conrads, T.P., Issaq, H.J., Veenstra, T.D. (2004). Two-dimensional liquid chromatography-capillary zone electrophoresis—sheathless electrospray ionization-mass spectrometry evaluation for peptide analysis and protein identification. Electrophoresis 25, 1973-1980. 

J. Gobom, M. Schuerenberg, M. Mueller, D. Theiss, H. Lehrach, and E. Nordhoff. a-Cyano-4-hydroxycinnamic Acid Affinity Sample Preparation. A Protocol for MALDI-MS Peptide Analysis in Proteomics. Anal. Chem., 73(2001) 434-438. 

J. R. Yates, D. Cociorva, L. Liao, and V. Zabrouskov. Performance of a Linear Ion Trap-Orbitrap Hybrid for Peptide Analysis. Anal. Chem., 78(2006) 493-500. 

Pennington, M.W. and Dunn, B.M. (Eds.) (1994) Peptide Synthesis Protocols. 350pp. Humana Press. (Also Peptide Analysis Protocols). 

DH Rich, J Singh. The carbodiimide method, in E Gross, J Meienhofer, eds. The Peptides Analysis, Synthesis, Biology, Academic, New York, 1979, Vol l,pp 241-261. 

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