Cannabinoid receptors gene expression

Chakrabarti, A., Ekuta, J.E., Onaivi, E.S. (1998) Neurobehavioral effects of anandamide and cannabinoid receptor gene expression in mice. Brain Res. Bull. 45, 67-74. 

Fig. 4. C57B1/6 mice were subjected to acute and chronic mild stress and assessed for anhedonia (CMS animals) by weekly sucrose consmnption. Mice were sacri eed at the end of wk 4 or following acute stress, and the brains were quickly dissected to extract RNA for CB2 cannabinoid receptor gene expression by real-time PCR system using the gene-speci c probe and primers (T aqMan real-time PCR system, ABl 7900). CB2 gene expression was detected in the acute and chronic mild stress mice in the striatum (str), midbrain (mid), and the hippocampus (hip).

Gadzicki, D., K. R. Muller-Vahl, D. Heller, et al. Tourette syndrome is not caused by mutations in the central cannabinoid receptor (CNRl) gene. Am J Med Genet 2004 127B(1) 97-103. Oliva, ]. M., S. Ortiz, T. Palomo, and ]. Manzanares. Spontaneous cannabinoid withdrawal produces a differential time-related responsiveness in cannabinoid GBl receptor gene expression in the mouse brain. J Psychopharmacol 2004 18(1) 59-65. 

Onaivi ES, Chakrabarti A, Gwebu ET, Chaudhuri G (1996) Neurobehavioral effects of A9-THC and cannabinoid (CBI) receptor gene expression in mice. Behav Brain Res 72 115-125... 

Abood ME, Ditto KA, Noel MA, Showalter VM, Tao Q (1997) Isolation and expression of mouse CBI cannabinoid receptor gene comparison of binding properties with those of native CBI receptors in mouse brain and N18TG2 neuroblastoma cells. Biochem Pharmacol 53 207-214... 

These anatomical data reinforce the notion that CB 1 receptors play an important role in the mediation of motor effects of cannabinoid agonists, an idea that is supported by results obtained when studying motor function in mice deficient in CBl receptor gene expression (Ledent et al. 1999 Zimmer et al. 1999). These knockout mice exhibited significant motor disturbances, although the two models developed so far have yielded conflicting results, since a trend to hyperlocomotion was observed in one of these two models (Ledent et al. 1999) and hypoactivity was evident in the other (Zimmer et al. 1999). 

Oliva JM, Ortiz S, Palomo T, Manzanares J (2004) Spontaneous cannabinoid withdrawal produces a differential time-related responsiveness in cannabinoid CBl receptor gene expression in the mouse brain. J Psychopharmacol 18 59-65... 

CMS and control mice in-groups of 5-10 were sacri ced and brains removed immediately to extract RNA. The expression of CB2 RNA was compared by real-time PCR system (TaqMan, ABI) using the CB2 cannabinoid receptor gene-speci c probe and primers. 

Previous study of the distribution of rat CB2 receptor mRNA was assessed by Northern blot analysis, and CB2 gene expression was detected only in the spleen and not in the brain, which led the investigators to conclude that CB2 receptor was absent in the CNS (14). Our preliminary data using real-time PCR system (TaqMan, ABI) with CB2 cannabinoid-receptor-gene-speciiic probe and primers indicated abundant expression of CB2 mRNA in the striatum, cortex, and hippocampus in naive and stressed mice. 

Jbilo O, Derocq J-M, Segui M, Le Fur G, Casellas P (1999) Sdmula-don of peripheral cannabinoid receptor CB2 induces MCP-1 and IL-8 gene expression in human promyelocydc cell line HL60. FEBS Lett 448 273-277. 

Steiner H, Bonner TI, Zimmer AM, Kitai ST, Zimmer A (1999) Altered gene expression in striatal projection neurons in CBi cannabinoid receptor knockout mice [see comments]. Proc Natl Acad Sci U S A 96 5786-5790... 

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