Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

C-terminal

Identify the N terminal and the C terminal amino acid m the original peptide and m each fragment... [Pg.1130]

An amino acid sequence is ambiguous unless we know the direction m which to read It—left to right or right to left We need to know which end is the N terminus and which IS the C terminus As we saw m the preceding section carboxypeptidase catalyzed hydrolysis cleaves the C terminal ammo acid and so can be used to identify it What about the N terminus ... [Pg.1131]

Protect the ammo group of the N terminal ammo acid and the carboxyl group of the C terminal ammo acid... [Pg.1137]

The actual process of solid phase peptide synthesis outlined m Figure 27 15 begins with the attachment of the C terminal ammo acid to the chloromethylated polymer m step 1 Nucleophilic substitution by the carboxylate anion of an N Boc protected C terminal... [Pg.1141]

By successively adding ammo acid residues to the C terminal ammo acid it took Memfield only eight days to synthesize bradykmm m 68% yield The biological activ ity of synthetic bradykmm was identical with that of natural material... [Pg.1142]

Step 2 The Boc protecting group is removed by treatment with hydrochloric acid m dilute acetic acid After the resin has been washed the C terminal ammo acid IS ready for coupling... [Pg.1143]

Step 3 The resin bound C terminal ammo acid IS coupled to an N protected ammo acid by using N N dicyclohexylcarbodiimide Excess reagent and N N dicyclohexylurea are washed away from the resin after coupling is complete... [Pg.1143]

FIGURE 27 19 Proposed mechanism of hydrolysis of a peptide catalyzed by carboxypeptidase A The peptide is bound at the active site by an ionic bond between its C terminal ammo acid and the positively charged side chain of arginine 145 Coordination of Zn to oxygen makes the carbon of the carbonyl group more positive and increases the rate of nucleophilic attack by water... [Pg.1147]

Carboxypeptidase catalyzed hydrolysis can be used to identify the C terminal ammo acid The N terminus is determined by chemical means One reagent used for this purpose is Sanger s reagent 1 fluoro 2 4 dimtrobenzene (see Figure 27 9)... [Pg.1151]

Solid phase peptide synthesis (Section 27 18) Method for peptide synthesis m which the C terminal ammo acid is co valently attached to an inert solid support and successive ammo acids are attached via peptide bond formation At the completion of the synthesis the polypeptide is removed from the support... [Pg.1293]

However, interpretation of, or even obtaining, the mass spectrum of a peptide can be difficult, and many techniques have been introduced to overcome such difficulties. These techniques include modifying the side chains in the peptide and protecting the N- and C-terminals by special groups. Despite many advances made by these approaches, it is not always easy to read the sequence from the mass spectrum because some amide bond cleavages are less easy than others and give little information. To overcome this problem, tandem mass spectrometry has been applied to this dry approach to peptide sequencing with considerable success. Further, electrospray ionization has been used to determine the molecular masses of proteins and peptides with unprecedented accuracy. [Pg.333]

Combination and disproportionation are competitive processes and do not occur to the same extent for all polymers. For example, at 60°C termination is virtually 100% by combination for polyacrylonitrile and 100% by disproportionation for poly (vinyl acetate). For polystyrene and poly (methyl methacrylate), both reactions contribute to termination, although each in different proportions. Each of the rate constants for termination individually follows the Arrhenius equation, so the relative amounts of termination by the two modes is given by... [Pg.360]

P-Endorphin. A peptide corresponding to the 31 C-terminal amino acids of P-LPH was first discovered in camel pituitary tissue (10). This substance is P-endorphin, which exerts a potent analgesic effect by binding to cell surface receptors in the central nervous system. The sequence of P-endorphin is well conserved across species for the first 25 N-terminal amino acids. Opiates derived from plant sources, eg, heroin, morphine, opium, etc, exert their actions by interacting with the P-endorphin receptor. On a molar basis, this peptide has approximately five times the potency of morphine. Both P-endorphin and ACTH ate cosecreted from the pituitary gland. Whereas the physiologic importance of P-endorphin release into the systemic circulation is not certain, this molecule clearly has been shown to be an important neurotransmitter within the central nervous system. Endorphin has been invaluable as a research tool, but has not been clinically useful due to the avadabihty of plant-derived opiates. [Pg.175]

These methodologies have been reviewed (22). In both methods, synthesis involves assembly of protected peptide chains, deprotection, purification, and characterization. However, the soHd-phase method, pioneered by Merrifield, dominates the field of peptide chemistry (23). In SPPS, the C-terminal amino acid of the desired peptide is attached to a polymeric soHd support. The addition of amino acids (qv) requires a number of relatively simple steps that are easily automated. Therefore, SPPS contains a number of advantages compared to the solution approach, including fewer solubiUty problems, use of less specialized chemistry, potential for automation, and requirement of relatively less skilled operators (22). Additionally, intermediates are not isolated and purified, and therefore the steps can be carried out more rapidly. Moreover, the SPPS method has been shown to proceed without racemization, whereas in fragment synthesis there is always a potential for racemization. Solution synthesis provides peptides of relatively higher purity however, the addition of hplc methodologies allows for pure peptide products from SPPS as well. [Pg.200]

The group of peptides known as tachykinins include substance P, substance K or neurokinin A, and neuromedin K, ie, neurokinin B, as well as a number of nonmammalian peptides. All members of this family contain the conserved carboxy-terrninal sequence Phe-X-Gly-Leu-Met-NH2, where X is an aromatic, ie, Phe or Tyr, or branched aliphatic, eg, Val or lie, amino acid. In general, this C-terminal sequence is cmcial for tachykinin activity (33) in fact, both the methionineamide and the C-terminal amide are cmcial for activity. The nature of the X residue in this sequence determines pharmacological identity (34,35) thus the substance P group contains an aromatic residue in this position, while the substance K group contains an aliphatic residue (33). [Pg.202]

NifA proteins are of one of two types. One group consists of 02-sensitive NifA proteins, which are found in the rhizobia Bhodobacter and Ae spirillum. These proteins have a proposed iron-binding motif on a linker between the central and C-terminal domains that may form a redox-sensitive... [Pg.90]

H-Tyr-D-Ala-Pile-Asp-Val-Val-Gly-NH2 (D-Ala -deltorpliiu I) and H-Tyr-D-Ala-Plie-Glu-Val-Val-Gly-NH2 (D-Ala -deltorpliiu II) display greater 5-selectivity than DPDPE owiag to their higher 5-receptor affinity (96). These compounds both contain the same N-terminal tripeptide sequence as the. -selective dermorphins, which underscores the importance of the C-terminal tetrapeptide sequence in conferring 5-selectivity. [Pg.448]

The endogenous peptide dynorphin and its C-terminally degraded fragments dynorphin A 3 and dynorphin A2 c, are somewhat K-selective... [Pg.448]

A2 23, and D-Pro -dynorphin A 23 (97). Analogues with C-terminal deletions, such as D-Pro -dynorphin have been found to display further... [Pg.448]

Fig. 5. Schematic diagram of the presumed arrangement of the amino acid sequence for the 5-opioid receptor, showing seven putative transmembrane segments three intracellular loops, A three extracellular loops, B the extracellular N-terrninus and the intracellular C-terrninus, where (0) represents amino acid residues common to ] -, 5-, and K-receptors ( ), amino acid residues common to all three opioid receptors and other neuropeptide receptors and (O), other amino acids. Branches on the N-terruinal region indicate possible glycosylation sites, whereas P symbols in the C-terminal region indicate... Fig. 5. Schematic diagram of the presumed arrangement of the amino acid sequence for the 5-opioid receptor, showing seven putative transmembrane segments three intracellular loops, A three extracellular loops, B the extracellular N-terrninus and the intracellular C-terrninus, where (0) represents amino acid residues common to ] -, 5-, and K-receptors ( ), amino acid residues common to all three opioid receptors and other neuropeptide receptors and (O), other amino acids. Branches on the N-terruinal region indicate possible glycosylation sites, whereas P symbols in the C-terminal region indicate...
A two-site immunometric assay of undecapeptide substance P (SP) has been developed. This assay is based on the use of two different antibodies specifically directed against the N- and C-terminal parts of the peptide (95). Affinity-purified polyclonal antibodies raised against the six amino-terminal residues of the molecule were used as capture antibodies. A monoclonal antibody directed against the carboxy terminal part of substance P (SP), covalently coupled to the enzyme acetylcholinesterase, was used as the tracer antibody. The assay is very sensitive, having a detection limit close to 3 pg/mL. The assay is fiiUy specific for SP because cross-reactivity coefficients between 0.01% were observed with other tachykinins, SP derivatives, and SP fragments. The assay can be used to measure the SP content of rat brain extracts. [Pg.247]

Research on an hCG vaccine has been conducted over the past 15 years. WHO has conducted a phase I clinical study in AustraUa, using a vaccine based on a synthetic C-terminal peptide (109—141) of P-hCG conjugated to Diptheria Toxoid (CTP-DT), that showed potentially effective contraceptive levels of antibodies were produced in vaccinated women without any adverse side effects. Phase II clinical studies are under consideration to determine if the immune response, raised to its prototype anti-hCG vaccine, is capable of preventing pregnancy in fertile women volunteers (115). While research on the C-terminal peptide from the P-subunit of hCG has been carried out under the auspices of WHO, research supported by the Population Council and the National Institutes of Health has involved two alternative vaccine candidates (109,116,118). [Pg.123]


See other pages where C-terminal is mentioned: [Pg.29]    [Pg.404]    [Pg.1035]    [Pg.235]    [Pg.1126]    [Pg.1127]    [Pg.1127]    [Pg.1127]    [Pg.1129]    [Pg.1129]    [Pg.1129]    [Pg.1130]    [Pg.1133]    [Pg.1142]    [Pg.1147]    [Pg.330]    [Pg.181]    [Pg.190]    [Pg.198]    [Pg.203]    [Pg.204]    [Pg.241]    [Pg.548]    [Pg.549]    [Pg.447]    [Pg.450]    [Pg.253]    [Pg.259]    [Pg.219]   
See also in sourсe #XX -- [ Pg.28 ]

See also in sourсe #XX -- [ Pg.34 , Pg.35 , Pg.37 , Pg.38 , Pg.40 , Pg.65 ]

See also in sourсe #XX -- [ Pg.171 , Pg.203 , Pg.214 , Pg.215 , Pg.216 , Pg.244 , Pg.259 , Pg.283 , Pg.310 , Pg.324 ]

See also in sourсe #XX -- [ Pg.84 , Pg.125 , Pg.187 , Pg.203 ]

See also in sourсe #XX -- [ Pg.55 , Pg.78 ]

See also in sourсe #XX -- [ Pg.94 ]

See also in sourсe #XX -- [ Pg.667 ]

See also in sourсe #XX -- [ Pg.5 ]

See also in sourсe #XX -- [ Pg.300 , Pg.301 ]

See also in sourсe #XX -- [ Pg.70 ]

See also in sourсe #XX -- [ Pg.797 , Pg.807 , Pg.841 , Pg.900 , Pg.907 , Pg.913 , Pg.919 ]




SEARCH



Amides C-terminal

C terminal a-helix

C-Jun-N-terminal kinases

C-Jun-NH2-terminal kinase

C-Terminal Activation by Lipase

C-Terminal Tripeptide Mimetics

C-terminal Gly residue

C-terminal amidation

C-terminal amide moiety

C-terminal amino acid

C-terminal amino acid residu

C-terminal amino acid residues

C-terminal analysis

C-terminal carboxy group

C-terminal carboxyl group

C-terminal domain

C-terminal end

C-terminal fragment ions

C-terminal ladder sequencing

C-terminal modified peptides

C-terminal peptide sequencing

C-terminal regions

C-terminal repeat domain

C-terminal residue

C-terminal residue, determining

C-terminal sequence determination

C-terminal sequences

C-terminal sequencing

C-terminal structure

C-terminal tetrapeptide

C-terminal thiocarboxylate

C-terminal thioester

C-terminal transmembrane domains

C-terminal transmembrane segments

Cholecystokinin-C-terminal octapeptid

Enzymic determination of C-terminal sequences

Esterification of the C-terminal fragment on 2-chlorotrityl chloride resin

Formation of C-N Bonds via Anti-Markovnikov Addition to Terminal Alkynes

Leucine rich repeats C-terminal subdomain

Peptides C-terminal thioester

Protein C-terminal amino acid

RNA polymerase II C-terminal domain

The C-Terminal Domain

The Flexible C-Terminal Domain

Thioesterification via Activation of C-Terminal Carboxylic Acids

Ubiquitin C-terminal Hydrolases (UCH)

Ubiquitin C-terminal hydrolase

Ubiquitin C-terminal hydrolases

© 2024 chempedia.info