Lactose-peptone broth

The effects of ampicillin-induced spheroplast formation on the production of molecular hydrogen by Escherichia coli carrying out fermentation in a lactose-peptone broth with an osmolality of 342 mosmol/1 was investigated previously... 

To prepare the single-strength lactose-peptone broth, the nutrient broth described above is diluted with the same volume of demineralized water prior to the addition of the bromocresol purple indicator. The pH is then set and the indicator solution added. Test-tubes are then each filled with 10 ml of this solution a Durham tube is added to each test tube and the... 

This culture medium is prepared in exactly the same way as the preparation of the lactose-peptone broth (Recipe 3), the only difference being that glucose is used instead of lactose (same quantity). 

Sterile lactose-peptone broth in suitable vessels, double-strength for 100 ml and 10 ml water (broth I), single-strength for 1 ml and 0.1 ml water (broth II) with Durham tubes (see Recipe 3)... 

The first issue in bioburden determination that merits validation is the choice of fluid used in preparatory stages of removal of microorganisms from devices and for suspending, dissolving, and diluting dosage forms. Phosphate buffer pH 7.2. buffered sodium chloride-peptone solution pH 7.0, and lactose broth are recommended in the various compendia. Saline, Rtnger s solution, and... 

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