Bovine albumin with glucose

Reaction Mixtures of Bovine Serum Albumin with Glucose or Glyoxal in the Absence/Presence of Ascorbic Acid... 

Note First results for the determination of glucose in urine and cerebrospinal fluid (CSF) using this hexokinase method have been published (SI99, S224). In this case the samples should be diluted with 1% bovine albumin solution. Good correspondence to a comparative method was described for CSF (S224). A 30% deviation was known for urine samples (SI99). 

Figure 2. Pyro-GCMS output showing pattern of labeling peptidic groups in the soil incubated with glucose (expl described in text), HS C4abeled soil humic material. BSA Bovine serum albumin, a standard peptidic material.

In 1988, a pioneering work dealing with electrode characteristics of an immobilized enzyme chemically modified electrode based on bilayer-film coating for amperometric determination of glucose was reported. In this work, a substrate was coated with two kinds of polymeric films in a bilayer state, that is, first with the cobalt tetrakis(o-aminophenyl)porphyrin polymer film and then with the enzyme film consisting of bovine serum albumin and glucose oxidase that were... 

A stable enzyme-D-glucose intermediate has been obtained in the hydrolysis of methyl a-D-[ C]glucopyranoside by an a-D-glucosidase from Saccharomyces oviformis Phenol was used to terminate the reaction and to trap the intermediate in a rapid-flow technique. The intermediate appears to be covalently linked, since continuous washing of the denatured protein failed to remove the radiolabel, which was also retained by a tryptic peptide isolated by gel filtration. Treatment of the intermediate with acid released D-[ C]glucose. The radiolabel was not bound when the enzyme was replaced with bovine albumin or when jS-D-glucosylamine (a potent inhibitor of a-D-glucosidase) was added with the enzyme. 

Compound (60) was prepared as a carrier for introducing anti-hepatitis drugs, so that they concentrate on the hepatocytes, by reaction of bovine serum albumin with compound (61), and fluorescent diastereomeric 1-isoindolyl l-thio-/6-D-glucoside derivatives (62) were made from amino acids and q-phthalaldehyde in the presence of l-thio-/9-D-glucose for the HPLC resolution of enantiomeric amino acids. ... 

We begin with electrophoretic studies on undenatured proteins. Stastna, et al. report the mobility of bovine serum albumin and glucose-6-phosphate dehydrogenase through three polymers(34), as shown in Figure 3.13. The values of... 

Fig. 4. The effects of unsaturated fatty-acid depletion on the passive permeability of yeast mitochondria to protons. KDl 15 cells were grown in batch culture with glucose as the carbon source on medium supplemented with 3 mg/ml or 0.05 mg/ml of Tween 80 as a source of unsaturated fatty acid. Mitochondria were isolated from both batches of cells and were incubated (10 mg protein) aerobically at 30°C in 3.0 ml of medium containing KCl (125 mM), glycylglycine (2 mM, pW 7.0), EDTA (0.5 mM, pH 7.0), bovine serum albumin (2 mg/ml), and antimycin A (5 Mg) Carbonyl cyanideM-chlorophenylhydrazone (10/xM) and HCl (0.5 /tmol) were added as indicated. Proton absorption was measured as described by Haslam et The numbers above the curves represent the half times for passive equilibration of the protons.

Recently, two examples of the separation of enantiomers using CCC have been published (Fig. 1-2). The complete enantiomeric separation of commercial d,l-kynurenine (2) with bovine serum albumin (BSA) as a chiral selector in an aqueous-aqueous polymer phase system was achieved within 3.5 h [128]. Moreover, the chiral resolution of 100 mg of an estrogen receptor partial agonist (7-DMO, 3) was performed using a sulfated (3-cyclodextrin [129, 130], while previous attempts with unsubstituted cyclodextrin were not successful [124]. The same authors described the partial resolution of a glucose-6-phosphatase inhibitor (4) with a Whelk-0 derivative as chiral selector (5) [129]. 

X 10 2 M Tris HC1 buffer, pH 7.5, 2.4 X 10 3 M glucose 6-phosphate (G-6-P), 1.6 units glucose 6-phosphate dehydrogenase (G-6-P dH), 5.1 X 10"5 M NADP, and 2.7 X 10 3 M KC1. In addition, the following chemicals were included in the final concentration indicated 5.1 X 10-3 M NADH, 1% (W/V) bovine serum albumin (BSA), and 1.0 mg aldrin in 0.1 ml ethanol. Whole body homogenate experiments included all of the above chemicals unless otherwise noted. Reaction mixtures were incubated with swirling in test tubes at 30 - 1°C. Reactions in Steps 1-4 of the experimental sequence were stopped after 1 hr and Steps 6-8 after 15 min, by the addition of 2 ml 5% TCA. 

The effects of fouling were studied by obtaining glucose calibration curves for two ultramicrobiosensors, one without electropolymerized film, the other with poly(1,3-DAB). Both sensors were then placed in a solution containing 3% w/v bovine serum albumin, at temperature of 4°C, for 6 h. After 6 h, both sensors were again calibrated. 

The aim of this contribution is to present experimental data concerning the synthesis, physicochemical properties and bio-activity of several nanosilica-based composites tested by interaction with native red blood cells (RBCs). The investigations include (i) synthesis of nanocomposites by adsorption of BSA, fructose and glucose on a fumed silica surface, and (ii) analysis of the adsorption properties of composites and their bio-activity. It is known that both glucose and fructose do not individually adsorb on fumed silica therefore, bovine serum albumin was used as a mediator to bind the monosaccharides to the fumed silica surface. 

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