Glucose with bovine serum albumin

Recently, two examples of the separation of enantiomers using CCC have been published (Fig. 1-2). The complete enantiomeric separation of commercial d,l-kynurenine (2) with bovine serum albumin (BSA) as a chiral selector in an aqueous-aqueous polymer phase system was achieved within 3.5 h [128]. Moreover, the chiral resolution of 100 mg of an estrogen receptor partial agonist (7-DMO, 3) was performed using a sulfated (3-cyclodextrin [129, 130], while previous attempts with unsubstituted cyclodextrin were not successful [124]. The same authors described the partial resolution of a glucose-6-phosphatase inhibitor (4) with a Whelk-0 derivative as chiral selector (5) [129]. 

Two methods for the spatially controlled deposition of proteins on microelectrodes are described. The first technique involves the entrapment of glucose oxidase in photopolymerized polyHK. The second uses electrochemically aid adsorption to deposit urease and to co-deposit glucose oxidase with bovine serum albumin. Both techniques were found to lead to active deposits and the properties and optimisation of the deposition procedures will be described. Further, to facilitate glucose measurement in complex medi the depointion of a thin film of polypyrrole following that of the protdns is described. The properties of s film with respect to two model interferents and complex yeast extract medium will be reported. 

X 10 2 M Tris HC1 buffer, pH 7.5, 2.4 X 10 3 M glucose 6-phosphate (G-6-P), 1.6 units glucose 6-phosphate dehydrogenase (G-6-P dH), 5.1 X 10"5 M NADP, and 2.7 X 10 3 M KC1. In addition, the following chemicals were included in the final concentration indicated 5.1 X 10-3 M NADH, 1% (W/V) bovine serum albumin (BSA), and 1.0 mg aldrin in 0.1 ml ethanol. Whole body homogenate experiments included all of the above chemicals unless otherwise noted. Reaction mixtures were incubated with swirling in test tubes at 30 - 1°C. Reactions in Steps 1-4 of the experimental sequence were stopped after 1 hr and Steps 6-8 after 15 min, by the addition of 2 ml 5% TCA. 

The effects of fouling were studied by obtaining glucose calibration curves for two ultramicrobiosensors, one without electropolymerized film, the other with poly(1,3-DAB). Both sensors were then placed in a solution containing 3% w/v bovine serum albumin, at temperature of 4°C, for 6 h. After 6 h, both sensors were again calibrated. 

The aim of this contribution is to present experimental data concerning the synthesis, physicochemical properties and bio-activity of several nanosilica-based composites tested by interaction with native red blood cells (RBCs). The investigations include (i) synthesis of nanocomposites by adsorption of BSA, fructose and glucose on a fumed silica surface, and (ii) analysis of the adsorption properties of composites and their bio-activity. It is known that both glucose and fructose do not individually adsorb on fumed silica therefore, bovine serum albumin was used as a mediator to bind the monosaccharides to the fumed silica surface. 

In situ rat kidney perfusion experiments were carried out as described in Ref. [5.4]. Briefly, the perfusion medium used was Krebs-Henseleit buffer at pH7.4 containing glucose (5.6 mmol/L), 5.5% bovine serum albumin (fraction V, Sigma), 5-6% washed rat erythrocytes and different amino acids. After cannulation of the ureter and the renal vein and artery, the right kidney was perfused via the renal artery at a constant arterial pressure of 14.5 kPa at 37"C. After an equilibration period of 30-35 min, the agent was added and the perfusion continued for 60 min. Urine samples were collected every 10 min, and midpoint samples of the perfusate were also obtained. Inuhn was used as the standard for the measurement of the glomerular filtration rate. Elimination parameters of labelled peptides in the perfused rat kidney were characterized by the values of total renal clearance (CLr) and free fraction of the peptide in the perfusate (FJ. These values were compared with the glomerular filtration rate (GFR). 

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