Bone mineral levels, rats, effect

Studies in rats have shown effects of lead on bone mineralization and bone growth. The effects observed in rats may be relevant to our understanding of the mechanisms for the growth deficits that have been associated with low-level in utero and childhood lead exposures. Additional studies of the effects of lead on bone metabolism in humans and in animal models would improve our understanding of the toxicological significance of lead in bone. 

Williams DC, Paul DC, Black LJ (1991) Effects of estrogen and tamoxifen on serum osteocalcin levels in ovariectomized rats. Bone Miner 14 205-220... 

Recently, a Japanese research group published preclinical safety and efficacy data of an oral antiestrogen (TZE-5323) (Saito et al. 2003). This drug has been shown to have a strong affinity for human ERa and ER/i and a dose-dependent capacity to inhibit estradiol-stimulated transcriptional activation (Saito et al. 2003). In the experimental endometriosis model in rats, TZE-5323 dose-dependently reduced the volume of the endometrial implant with an effectiveness similar to that of danazol and leuprorelin acetate without causing significant changes in bone mineral density and in serum estradiol levels (Saito et al. 2003). 

The data presented in this paper indicate that excess levels (0.75%) of dietary zinc result in decreases in the bioavailability of calcium and phosphorus in rats and interfere with normal bone mineralization. High dietary levels of calcium or zinc appeared to cause a shift in the excretion of phosphorus from the urine to the feces, while the presence of extra phosphorus tended to keep the pathway of phosphorus excretion via the urine. The presence of large amounts of phosphorus in the Intestinal tract due to high intakes of zinc would increase the possibility of the formation of insoluble phosphate salts with various cations, including calcium, which may be present. A shift in phosphorus excretion from the feces to the urine, however, could result in an environmental condition within the system which would tend to increase the bioavailability of cations to the animal. The adverse effect of zinc toxicity on calcium and phosphorus status of young rats could be alleviated with calcium and/or phosphorus supplements. 

Westerlind KC, Turner RT.The skeletal effects of spaceflight in growing rats Tissue specific alterations in mRNA levels for TGF-b. J Bone Miner Res. 1995 10 843-848. 

The use of selective ERa modulators has provided information similar to that obtained from ERKO transgenic mice. PPT stimulated uterine proliferation as well as ethinyl-estradiol, prevented bone mineral density loss in ovariectomized rats, lowered serum cholesterol levels and prevented hot flashes in a chemically induced hot flash rat model [17]. The use of PPTalso showed that ERa is primarily responsible for the effects of estrogen on the pituitary, vasodilation, neuroprotection, cardioprotec-tion and insulin sensitivity (reviewed in Ref. [18]). Protective effects were also... 

Manjhi J, Mathur R, Behari J (2010) Effect of low level capacitive-coupled pulsed electric field stimulation on mineral profile of weightbearing bones in ovariectomized rats. J Biomed Mater Res B Appl Biomater 92 189-195. 

Table II shows the effects of varying dietary levels of zinc on weight gains and on bone calcium and phosphorus levels of young rats at the end of a 4-week experiment. Increases in dietary zinc were associated with significant linear decreases in bone calcium and phosphorus deposition. The bones taken from animals at the time of sacrifice and used for the mineral analyses were very soft in nature and could be easily squeezed with the fingers.

Yerramshetty, J.S., Lind, C., and Akkus, O. (2006) The compositional and physicochemical homogeneity of male femoral cortex increases after the sixth decade. Bone, 39 (6), 1236-1243. Donnelly, E. et al. (2010) Effects of tissue age on bone tissue material composition and nanomechanical properties in the rat cortex. J. Biomed. Mater. Res. A, 92 (3), 1048-1056. Donnelly, E. et al (2010) Contribution of mineral to bone structural behavior and tissue mechanical properties. Calcif. Tissue Int., 87 (5), 450—460. Pathak, S. et al (2012) Assessment of lamellar level properties in mouse bone utilizing a novel spherical nanoindentation data analysis method. J. Mech. Behav. Biomed. Mater., 13, 102—117. Burket, J.C. et al (2013) Variations in nanomechanical properties and tissue composition within trabeculae from an ovine model of osteoporosis and treatment. Bone, 52 (1), 326-336. Carden, A. et al (2003) Ultrastructural changes accompanying the mechanical deformation of bone tissue a Raman imaging study. Calcif. Tissue Int., 72 (2), 166-175. 

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