Bone mesenchymal stem cells

Ke, Y., Wang, Y.J., Ren, L., Zhao, Q.C., and Huang, W. (2010) Modified PHBV scaffolds by in situ UV polymerization structural characteristic, mechanical properties and bone mesenchymal stem cell compatibility. Acta Biomater., 6 (4), 1329-1336. 

HA can be added to the surface of nanofibrous PLLA scaffolds through its inclusion into the PLLA solution prior to phase separation.The presence of HA in the material resulted in an increase in the compressive modulus, giving a value of 420 110 kPa for materials produced from an 80 wt% HA solution. As previously discussed, PLLA scaffolds with nanofibrous pore walls were able to adsorb higher levels of serum proteins than their solid wall counterparts. The addition of HA to the surface of the material was found to further enhance the protein adsorption from BSA when compared to a nanofibrous scaffold. The viability of bone mesenchymal stem cells (BMSCs) cultured on the nanofibrous PLLA scaffolds was determined by MTT assay after seven days. The viability of BMSCs was found to be greatest on the scaffold containing HA. ... 

Figure 7.3. All skeletal tissues arise from a single cell type, the mesenchymal stem cell. Differentiation into bone, cartilage, muscle, or ligament occurs in response to the mechanical and biochemical stimuli of the stem cell s environment.

The primary advantage of MSC for utilization in cell therapy is the ease with which they can be harvested from the bone marrow, isolated by plastic adherence, expanded in culture, genetically engineered, differentiated, and handled in vitro. The ease with which mesenchymal stem cells can be iron-dextran labeled (Ittrich et ah, 2005 Arab et al, 2004) and monitored by MRl... 

Mesenchymal stem cells are CD45 CD34 bone marrow cells that can be readily grown in culture. They are rare in the bone marrow (<0.01% of... 

Bruder SP, Kurth AA, Shea M, Hayes WC, Jaiswal N, Kadiyala S. Bone regeneration by implantation of purified, culture-expanded human mesenchymal stem cells. J Orthop Res 1998 16 155-162. 

Barbash IM, Chouraqui P, Baron J, Eeinberg MS, Etzion S, Tessone A, Miller R, Guetta E, Zipori D, Kedes LH, Kloner RA, Leor J. Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted myocardium feasibility, cell migration, and body distribution. Circulation 2003 108 863-868. 

Chen SL, Fang WW, Ye F, Liu YH, Qian J, Shan SJ, Zhang JJ, Chunhua RZ, Liao LM, LinS, Sun JP. Effect on left ventricular function of intracoronary transplantation of autologous bone marrow mesenchymal stem cell in patients with acute myocardial infarction. Am J Cardiol 2004 94 92-95. 

Duan HF, Wu CT, Wu DL, Lu Y, Liu HJ, Ha XQ, Zhang QW, Wang H, Jia XX, Wang LS. Treatment of myocardial ischemia with bone marrow-derived mesenchymal stem cells overexpressing hepatocyte growth factor. Mol Ther 2003 8 467-474. 

J. M. Zimmet and J. M. Hare. Emerging Role For Bone Marrow Derived Mesenchymal Stem Cells In Myocardial Regenerative Therapy. Basic Res Cardiol 2005 100 471-A81. 

Miao, Z., Jin, J., Chen, L., et al. (2006), Isolation of mesenchymal stem cells from human placenta Comparison with human bone marrow mesenchymal stem cells, Cell. Biol. Int., 30(9), 681-687. 

Kern, S., Eichler, H., Stoeve, J., Kluter, H., and Bieback, K. (2006), Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue, Stem Cells, 24(5), 1294-1301. 

N., Peters, C., Aubourg, P, et al. (1995), Bone marrow-derived mesenchymal stem cells remain host-derived despite successful hematopoietic engraftment after allogeneic transplantation in patients with lysosomal and peroxisomal storage diseases. Exp. Hematol, 27(11), 1675-1681. 

Wagner, W., Wein, F., Seckinger, A., et al. (2005), Comparative characteristics of mesenchymal stem cells from human bone marrow, adipose tissue, and umbilical cord blood, Exp. Hematol., 33(11), 1402-1416. 

Panepucci, R. A., Siufi, J. L., Silva, W. A., Jr., et al. (2004), Comparison of gene expression of umbilical cord vein and bone marrow-derived mesenchymal stem cells, Stem Cells, 22(7), 1263-1278. 

Robinson, S. N., Ng, J., Niu, T., et al. (2006), Superior ex vivo cord blood expansion following co-culture with bone marrow-derived mesenchymal stem cells, Bone Marrow Transplant., 37(4), 359-366. 

Mesenchymal stem cells (MSCs) are multipotent cells that contribute to the regeneration of mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon, adipose and marrow stroma [394517], [656539]. MSCs represent an important cellular component of the BM microenvironment [656703] and can be easily isolated from the adult BM stroma, where they represent a rare population of the cells (estimated at 0.001 to 0.01% of the nucleated cells, 10-fold less abundant than HSCs) [658632], [658640]. MSCs have also been found in umbilical cord blood, but not peripheral blood [658631]. Once isolated, MSCs can be expanded in culture through many generations, producing billions of MSCs for cellular therapy [656543]. 

Title Enhancing bone marrow engraftment using mesenchymal stem cells (MSCs). 

Phenotype of human mesenchymal stem cells isolated directly from bone marrow Davis-Sproul, J., McNeil, R., Simonetti, D., Craig, S., Moseley, A., Deans, R., Moorman, M. (1999). Blood, 94 10 Suppl 1 Abs 3905. 

Zahanich I, Graf EM, Heubach JF, Hempel U, Boxberger S, Ravens U. 2005. Molecular and functional expression of voltage-operated calcium channels during osteogenic differentiation of human mesenchymal stem cells. J Bone Miner Res 20 1637-46. 

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