Serum proteome

Adkins, J.N., Vamum, S.M., Auberry, K.J., Moore, R.J., Angell, N.H., Smith, R.D., Springer, D.L., Pounds, J.G. (2002). Toward a human blood serum proteome analysis by multidimensional separation coupled with mass spectrometry. Mol. Cell. Proteomics 1,47-955. 

E.F. Petricoin and L.A. Liotta, SELDI-TOF-based serum proteomic pattern diagnostics for early detection of cancer. Curr. Opin. Biotech. 15, 24-30 (2004). 

In addition to MALDI-TOF and LC-MS/MS, SELDI-TOF-MS can also be used to determine expression profiling of various biological samples, such as serum or plasma for early detection of infection. Serum proteomic profiling assay, for instance, has been used to distinguish patients with acute SARS (severe acute respiratory syndrome) from patients with fever and influenza with 100% accuracy [16]. A major limitation of SELDI-TOF-MS, however, is that it cannot be used for direct amino acid sequence identification of the biomarker proteins, necessitating further sample fractionation and protein purification. 

Conrads TP, Fusaro VA, Ross S, et al. High-resolution serum proteomic features for ovarian cancer detection. Endocr Relat Cancer 2004 11(2) 163 178. 

Rosenblatt KP, Bryant-Greenwood P, Killian JK, et al. Serum proteomics in cancer diagnosis and management. Annu Rev Med 2004 55 97-112. 

Yu LR, Zhou M, Conrads TP, Veenstra TD. Diagnostic proteomics Serum proteomic patterns for the detection of early stage cancers. Dis Marker 2003 19(4-5) 209-218. 

Ahmed N, Barker G, Oliva K, et al. An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum. Proteomics 2003 3(10) 1980-1987. 

Pieper R, Gatlin CL, Makusky AJ, Russo PS, Schatz CR, Miller SS, et al. The human serum proteome Display of nearly 3700 chromatographically separated protein spots on two-dimensional electrophoresis gels and identification of 325 distinct proteins. Prote-omics 2003 3(7) 1345 1364. 

Velassco, A., Trucco, C., Wiegand, L., Wood, K., et al. (2002a). Serum proteomic patterns for detection of prostate cancer. J. Natl. Cancer Inst. 94, 1576-1578. 

Pattern analysis of serum proteome distinguishes renal cell carcinoma from... 

Pang RT, Poon TC, Chan KC, Lee NL, Chiu RW, Tong YK, Wong RM, Cliim SS, Ngai SM, Sung JJ, Lo YM (2006) Serum Proteomic Fingerprints of Adult Padents with Severe Acute Respiratory Syndrome. Clin Chem 52(3) 421 29. 

Puroliit S, Podolsky R, Schatz D, Muir A, Hopkins D, Huang YH, She JX (2006) Assessing tire utility of SELDI-TOF and model averaging for serum proteomic biomarker discovery. Proteomics 6(24) 6405-6415. 

Sheng S, Chen D, van Eyk JE (2006) Multidimensional liquid chromatography separadon of intact proteins by chromatographic focusing and reversed phase of the human serum proteome Optimizadon and protein database. Mol Cell Proteomics 5 26-34. 

Coombes KR, Morris JS, Hu J, Edmonson SR, Baggerly KA (2005) Serum proteomics profiling—a young technology begins to mature. Nat Biotechnol 23 291-292. 

Lee, S.W. Pattern analysis of serum proteome distinguishes 28. renal cell carcinoma from other urologic diseases and healthy persons. Proteomics 2003, 5 (12), 2310-2316. 

Petricoin EF, III. Ornstein DK, Paweletz CP, Ardekani A, Hackett PS, Hitt BA, Velassco A, Trucco C, Wiegand L, Wood K, Simone CB, I. cvinc PJ, Linehan WM, Emmert-Buck MR, Steinberg SM, Kohn EC, Liotta LA. Serum proteomic patterns for detection of prostate cancer. J Natl Cancer Inst 2002 94(20) 1576-8. 

Tirumalai RS, Chan KC, Prieto DA, Issaq HJ, Conrads TP, Veenstra TD. Characterization of the low molecular weight human serum proteome. Mol Cell Proteomics 2003 2(10) 1096-103. 

Diamandis EP. Serum proteomic patterns for detection of prostate cancer. J Natl Cancer Inst 2003 95 489-90. 

Zhang Z, Bast RC, Yu Y, Li J, SokoH LJ, Rai AJ, et al. Three biomarkers identified from serum proteomic analysis for the detection of early state ovarian cancer. Cancer Res 2004 64 5882-90. 

Wong N, et al. Prediction of Ever fibrosis and cirrhosis in chronic hepatitis B infection by serum proteomic fingerprinting A pilot study. Clin Chem 2005 51 In Press. 

---