Binding mouse

Segal, D.M., et al. The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen-binding site. Proc. Natl. Acad. Sci. USA 71 4298-4302, 1974. 

M-1, but is higher for polymers and immune complexes of IgG. There is considerable class/subclass and species specificity among different FcR s. For example, the FcR on some human cells was found to bind mouse monoclonal lgG2a and lgG3 but not other IgG subclasses (9). Goat sera do not react with FcR s of human leucocytes (10). 

The ability for lectins to agglutinate some cell lines has suggested that insolubilized lectins may prove of value in certain specific cell separations. Edelman, Rutishauser and Millette [141], for example, showed that BALB mouse erythrocytes, thymocytes and lymphocytes bind to insolubilized Con A. While Zabriskie, Ollis and Berger found that wheat germ agglutinin insolubilized on polyacrylamide was able to bind mouse leukemia cells [142]. 

Omatsky et al. have designed bioassays with metal-encoded beads functionalized in such a way that COOH groups are exposed to covalently bind mouse IgGs. Their detection is carried out through goat anti-mouse antibody conjugated to a lanthanide group (45). 

Activation and nucleocytoplasmic translocation of CAR have been studied extensively. CAR is normally localized in the cytoplasm of liver cells. When treated with PB, CAR is translocated to the nucleus. This is the first step of activation of CAR in response to drug treatment. PB does not bind to the ligand binding domain (LBD) of human or mouse CAR. TCPOBOP binds mouse but not human CAR. In general, active transport of nuclear receptors from the cytoplasm to the nucleus requires a short sequence called the nuclear localization signal (NLS) that is exposed... 

Immunoaffinity chromatography utilizes the high specificity of antigen—antibody interactions to achieve a separation. The procedure typically involves the binding, to a soHd phase, of a mouse monoclonal antibody which reacts either directly with the protein to be purified or with a closely associated protein which itself binds the product protein. The former approach has been appHed in the preparation of Factor VIII (43) and Factor IX (61) concentrates. The latter method has been used in the preparation of Factor VIII (42) by immobilization of a monoclonal antibody to von WiHebrand factor [109319-16-6] (62), a protein to which Factor VIII binds noncovalenfly. Further purification is necessary downstream of the immunoaffinity step to remove... 

Zheng, Z.-H., Catano, A. R., Segelke, B.W., Stura, E.A., Peterson, P.A., Wilson, l.A. Crystal stmcture of mouse CDl an MHC-like fold with a large hydrophobic binding groove. Science 277 339-345, 1997. 

Desmoplakin is the most abundant desmosomal component that plays a critical role in linking intermediate filament networks to the desmosomal plaque. Desmoplakin forms rod-like dimers that bind to intermediate filaments and to the cadherin-associated proteins plakoglobin and plakophilin. Gene knock-out experiments have revealed an essential role of desmoplakin in establishing cell-cell contacts in early mouse embryos. 

A peroxisome proliferator-activated receptor (PPAR) binding site was identified in the murine FATP1 promoter. Several reports have shown a positive regulation of mouse FATPs by ligands that activate PPAR-a, PPAR-y, or PPAR-y/RXR heterodimers. 

The importance of cross-talk in GR actions is indicated by the construction of a GR dimerisation-deficient mutant mouse in which GR is unable to dimerise and therefore bind to DNA, thus separating the DNA-binding (transactivation) and inflammatory gene repression (transrepression) activities of glucocorticoids. In these animals dexamethasone was able to inhibit AP-1- and NF-kB-mediated gene transcription,... 

Monoclonal antibodies (mAh) are molecules that recognize and bind a specific foreign substance called an antigen. They are produced from a single clone of B lymphocytes. Conventionally, mouse mAh have been generated for experimental and diagnostic use. Techniques have been developed to humanize mouse mAh to facilitate their therapeutic use in humans. It is also now possible to make mAh which are fully human. 

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