Lectins, insolubilized

An alternative approach involves insolubilized glycoproteins. Thus, an affinity system for the isolation of the lectin of red kidney-bean (Phaseolus vulgaris)149,154 involved thyroglobulin-Sepharose, and, for... 

Enzyme purification Binding proteins and receptor sites Insolubilized nucleotides and nucleic acids Insolubilized lectins Immunochemical applications... 

The application of insolubilized lectins in polysaccharide fractionation was demonstrated by Kennedy and Rosevear with Con A-Sepharose... 

The ability for lectins to agglutinate some cell lines has suggested that insolubilized lectins may prove of value in certain specific cell separations. Edelman, Rutishauser and Millette [141], for example, showed that BALB mouse erythrocytes, thymocytes and lymphocytes bind to insolubilized Con A. While Zabriskie, Ollis and Berger found that wheat germ agglutinin insolubilized on polyacrylamide was able to bind mouse leukemia cells [142]. 

Plants contain at least three classes of hydroxyproline-rich glycoproteins 1) the cell wall proteins, 2) the arabinogalactan proteins, and 3) lectins. According to Cooper and Varner (23) the hydroxyproline-rich glycoprotein of the cell wall of carrots is secreted from the cytoplasm as a soluble monomer that slowly becomes insolubilized. 

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