Bases buffer solutions

Because the ionic product of water = [H ] [OH ] = 1.04 x 10" at 25°C, it follows that pH = 14 - pOH. Thus, a neutral solution (e.g., pure water at 25°C) in which [H j = [OH ] has a pH = pOH = 7. Acids show a lower pH and bases a higher pH than this neutral value of 7. The hydrogen ion concentrations can cover a wide range, from -1 g-ion/liter or more in acidic solutions to -lO" " g-ion/liter or less in alkaline solutions [53, p. 545]. Buffer action refers to the property of a solution in resisting change of pH upon addition of an acid or a base. Buffer solutions usually consist of a mixture of a weak acid and its salt (conjugate base) or of a weak base and its salt (conjugate acid). 

TABLE 4 Fluorescence Lifetimes, Relative Amplitudes and Normalized Fluorescence Quantum Yields of 124-kDa Pr Phytochrome from Oat in H2O- and D2O-Based Buffer Solutions [86 ... 

A buffer solution is a solution that resists a change in pH after addition of small amounts of an acid or a base. Buffer solutions require the presence of an acid to neutralize an added base and also the presence of a base to neutralize an added acid. These two components present in the buffer also must not neutralize each other A conjugate acid-base pair is present in buffers to fulfill these requirements. 

It was assumed that brine pH values obtained using a conventional pH electrode standardized in distilled water based buffer solutions would provide pH values of sufficient accuracy... 

For the dioxin binder screening, some changes were made as shown in Fig. 8.6. A new pentapeptide library was prepared because a strong affinity is required, to detect dioxins at the ppb level. The design of the screening solution is very important since it determines which peptides can be screened. Also, the composition of the screening solution may restrict the detection conditions of the sensor. For the herbicide binder screening, ethanol was used to dissolve the herbicide in an aqueous buffer. The refractive index of ethanol (1.3623) is not very different from that of water (1.3335) therefore, the ethanol-based buffer solution could be used in the... 

This has been a subject of much discussion and debate. Many assay kits will be provided with calibration standards that have been prepared in a surrogate matrix. One example is protein-based buffer solution. 

T. Imato, C. Azemori, Y. Asano, and N. Ishibashi, Flow Injection Analysis of Organic Acids and Amino Acids in Sake (Japanese Rice Wine) by Using pH-Sensitive Glass Electrode and Acid-Base Buffer Solution [in Japanese]. J. Flow Injection Anal., 3(2) (1986) 103. 

In a weak base buffer solution, when the concentrations of the weak base and its conjugate acid are equal the pOH of the solution is equal to the pKb of the weak base. 

Okafo, G N., Brown, R., and CamUleri, R Some physico-chemical properties that make D.O-based buffer solutions useful media for capUlary electrophoresis, J. Chem. Soc. Chem. Commun., 864,1991. 

EXAMPLE 19-3 Weak Base/Salt of Weak Base Buffer Solution... 

Buffer Solutions A buffer solution contains a weak acid and a salt derived from the acid. To maintain a relatively constant pH, the acid and base components of the buffer solution react with added acid or base. Buffer solutions play an important role in many chemical and biological processes. 

Capillary Electrochromatography Another approach to separating neutral species is capillary electrochromatography (CEC). In this technique the capillary tubing is packed with 1.5-3-pm silica particles coated with a bonded, nonpolar stationary phase. Neutral species separate based on their ability to partition between the stationary phase and the buffer solution (which, due to electroosmotic flow, is the mobile phase). Separations are similar to the analogous HPLC separation, but without the need for high-pressure pumps, furthermore, efficiency in CEC is better than in HPLC, with shorter analysis times. 

Since the principal hazard of contamination of acrolein is base-catalyzed polymerization, a "buffer" solution to shortstop such a polymerization is often employed for emergency addition to a reacting tank. A typical composition of this solution is 78% acetic acid, 15% water, and 7% hydroquinone. The acetic acid is the primary active ingredient. Water is added to depress the freezing point and to increase the solubiUty of hydroquinone. Hydroquinone (HQ) prevents free-radical polymerization. Such polymerization is not expected to be a safety hazard, but there is no reason to exclude HQ from the formulation. Sodium acetate may be included as well to stop polymerization by very strong acids. There is, however, a temperature rise when it is added to acrolein due to catalysis of the acetic acid-acrolein addition reaction. 

Because they are weak acids or bases, the iadicators may affect the pH of the sample, especially ia the case of a poorly buffered solution. Variations in the ionic strength or solvent composition, or both, also can produce large uncertainties in pH measurements, presumably caused by changes in the equihbria of the indicator species. Specific chemical reactions also may occur between solutes in the sample and the indicator species to produce appreciable pH errors. Examples of such interferences include binding of the indicator forms by proteins and colloidal substances and direct reaction with sample components, eg, oxidising agents and heavy-metal ions. 

Aqueous solutions of citric acid make excellent buffer systems when partially neutralized because citric acid is a weak acid and has three carboxyl groups, hence three p-K s. At 20°C pifj = 3.14, pi 2 4.77, and = 6.39 (2). The buffer range for citrate solutions is pH 2.5 to 6.5. Buffer systems can be made using a solution of citric acid and sodium citrate or by neutralizing a solution of citric acid with a base such as sodium hydroxide. In Table 4 stock solutions of 0.1 Af (0.33 N) citric acid are combined with 0.1 Af (0.33 N) sodium citrate to make a typical buffer solution. 

The effeet of temperature satisfies the Arrhenius relationship where the applieable range is relatively small beeause of low and high temperature effeets. The effeet of extreme pH values is related to the nature of enzymatie proteins as polyvalent aeids and bases, with aeid and basie groups (hydrophilie) eoneentrated on the outside of the protein. Einally, meehanieal forees sueh as surfaee tension and shear ean affeet enzyme aetivity by disturbing the shape of the enzyme moleeules. Sinee the shape of the aetive site of the enzyme is eonstrueted to eoirespond to the shape of the substrate, small alteration in the strueture ean severely affeet enzyme aetivity. Reaetor s stirrer speed, flowrate, and foaming must be eontrolled to maintain the produetivity of the enzyme. Consequently, during experimental investigations of the kineties enzyme eatalyzed reaetions, temperature, shear, and pH are earefully eontrolled the last by use of buffered solutions. 

Usually special cases of the full scheme are studied so that only one or two relaxation times are observed. Important examples are a solution of an acid-base solute in the presence of an acid-base indicator, and a buffered solution of an acid—base solute. PP... 

Buffers are solutions that tend to resist changes in their pH as acid or base is added. Typically, a buffer system is composed of a weak acid and its conjugate base. A solution of a weak acid that has a pH nearly equal to its by definition contains an amount of the conjugate base nearly equivalent to the weak acid. Note that in this region, the titration curve is relatively flat (Figure 2.15). Addition of H then has little effect because it is absorbed by the following reaction ... 

Textbooks of analytieal ehemistry should be eonsulted for further details eoneeming the ionization of weak aeids and bases and the theory of indieators, buffer solutions, and aeid-alkali titrations. " ... 

Calcium Chloride [25]. Calcium chloride estimation is based on calcium titration. To 20 ml of 1 1 mixture of toluene (xylene) isopropyl alcohol, add a 1-ml (or 0.1-ml, if calcium is high) sample of oil-base mud, while stirring. Dilute the mixture with 75 to 100 ml of distilled water. Add 2 ml of hardness buffer solution and 10 to 15 drops of hardness indicator solution. Titrate mixture with standard versenate solution until the color changes from wine-red to blue. If common standard versenate solution (1 ml = 20 g calcium ions) is used, then... 

This is what is supposed to happen with buffer solutions. Addition of strong acid lowers the pH slightly addition of strong base raises it slightly. 

The concentration of the acid is usually of the order 0.05-0.2 mol L" Similar remarks apply to weak bases. It is clear that the greater the concentrations of acid and conjugate base in a buffer solution, the greater will be the buffer capacity. A quantitative measure of buffer capacity is given by the number of moles of strong base required to change the pH of 1 litre of the solution by 1 pH unit. 

The preparation of a buffer solution of a definite pH is a simple process once the acid (or base) of appropriate dissociation constant is found smhll variations in pH are obtained by variations in the ratios of the acid to the salt concentration. One example is given in Table 2.2. 

This shows that the pM value of the solution is fixed by the value of K and the ratio of complex-ion concentration to that of the free ligand. If more of M is added to the solution, more complex will be formed and the value of pM will not change appreciably. Likewise, if M is removed from the solution by some reaction, some of the complex will dissociate to restore the value of pM. This recalls the behaviour of buffer solutions encountered with acids and bases (Section 2.20), and by analogy, the complex-ligand system may be termed a metal ion buffer. 

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