Bag proteins

Bag proteins differ from GrpE proteins by virtue of their ability to associate with ligands other than Hsp70 proteins. Although not shown experimentally, it is generally assumed that Bag proteins use this ability... 

Patient case A patient s daily nutritional requirements have been estimated to be 100 g protein and 2,000 total kcal. The patient has a central venous access and reports no history of hyperlipidemia or egg allergy. The patient is not fluid restricted. The PN solution will be compounded as an individualized regimen using a single-bag, 24-hour infusion of a 2-in-1 solution with intravenous fat emulsion (IVFE) piggybacked into the PN infusion line. Determine the total PN volume and administration rate by calculating the macronutrient stock solution volumes required to provide the desired daily nutrients. The stock solutions used to compound this regimen are 10% crystalline amino acids (CAA), 70% dextrose, and 20% IVFE. 

Yam tubers of Dioscorea alata (Umudike cultivar), D. rotundata (asukwu and obiaturugo cultivars)" and D. cayenensis (water yam and Nkokpu cultivars) were obtained from the National Root Crops Research Institute, Umudike, Nigeria. Some tubers were stored 6 or 12 months at room temperature (25-27 °C), some in vacuum dessicators over a suitable dessicant, and some in paper bags placed in a dark cabinet (absence of circulating air). Fresh tubers were peeled by carefully scraping away the cork layer to minimize loss of outer tissue since much of the protein is concentrated here ( ). They were then cut into 2 cu. cm. pieces, quickly frozen with solid CO2 in 50 9 portions in plastic bags, and stored in a freezer until needed. 

The greater number of folds in larger proteomes is intuitively obvious simply because the functioning of more complex organisms is expected to require a greater structural diversity of proteins. From a different perspective, the increase of diversity follows from a stochastic model, which describes a proteome as a finite sample from an infinite pool of proteins with a particular distribution of fold fractions ( a bag of proteins ). A previous random simulation analysis suggested that the stochastic model significantly (about twofold) underestimates the number of different folds in the proteomes (Wolf et al., 1999). In other words, the structural diversity of real proteomes does not seem to follow... 

At the end of the dialysis procedure, the bag is blotted dry and enzyme solution is removed prior to assessment of activity. It may be necessary to include cofactor in the assay mixture if it is possible that a dissociable cofactor was lost from the enzyme during dialysis. As the volume containing enzyme inside the dialysis bag changes to some degree during the dialysis procedure, it is usually necessary to correct measured enzyme activity to reflect this change in volume, and a correction based on protein concentration is often done in this regard. It is normal for activity thus measured to be expressed as a fraction of that in a parallel (dialyzed) control experiment. 

Polymer-based biomaterials are becoming increasingly important, whether they are used as medical supplies (pipes, catheters, bags), prostheses, or dental materials, or in a pharmaceutical context as drug conjugates [4, 7, 158-160], protein conjugates [6, 158, 159, 161], synthetic vectors [12, 14, 18, 162, 163], or as immuno-adjuvants [164, 165]. 

Effect of predigestion factors on the apparent digestibility of protein for swine determined by the mobile nylon bag technique. Journal of Animal Science 66 (8), 1963-1968. 

Moore-Colyer, M.J.S., Hyslop, J.J., Longland, A.C. and Cuddeford, D. (1997b) The degradation of organic matter and crude protein of four botanically diverse feed-stuffs in the foregut of ponies as measured by the mobile bag technique. In Proceedings of the British Society of Animal Science, Scarborough, p. 120. 

Huang, Y., Cai, J., Ji, L. and Li, Y. (2004) Classifying G-protein coupled receptors with bagging classification tree. Comput. Biol. Chem 28, 275-280. 

Water extract of W. sativa was prepared, dried and powdered. Powder was dissolved in phosphate buffer saline (pH 6.4) and centrifuged at 10.000 rpm for 30 min at 4 °C. The supernatant was collected as the soluble extract by removing the oily layer and unsoluble pellet. Protein concentration of the soluble extract was determined with Bradford method. Then proteins dialyzed against 0.05 M phosphate buffer (pH 6.4) using 3500 MW cut off dialyzing bags and centrifuged. 

---