Bacterial growth inhibition

Some related antibacteiials are also included with the sulfonamides. The azo dye, Piontosil (3) is metabolized to sulfanilamide in and was the piogenitoi of the sulfa dmgs. Also, the antibacteiial sulfones, eg, dapsone (4), are believed to act in a similai fashion on enzymes involved with synthesis of fohc acid, leading to bacterial growth inhibition. 

Bacterial signal peptidase is an example of a known enzyme that could serve as a target for new antibacterial agents [13], Recently, a catalytically active, soluble fragment of signal peptidase from E. coli has been crystallized as a complex with a P-lactam inhibitor [69], This represents a major step in the efforts toward the rational design of inhibitors that can be readily tested for their enzyme inhibition and bacterial growth-inhibition activities. 

Brought to logical conclusion, it was shown that the probit transformations of bacterial growth inhibition and inhibition of DNA biosynthesis by Nitroakridin 3582 were superposable while the same functions for inhibition of RNA and protein biosyntheses were superposable upon each other but indicated a lesser susceptibility of the test organism. This led to the conclusion that the mode of antibacterial action of the nitroacridine was its inhibition of DNA biosynthesis27. ... 

Microbiologic culture studies are useful fc>r bacterial identification, especially when an ocular infection foils to respond to treatment. Cultures are often obtained from the eyelids, the conjimctiva, expressed material from the lacrimal sac, and the cornea. Because preserved ophthalmic anesthetics have a bacteriostatic effect, cultures should be obtained if possible before anesthetic instillation. In the case of corneal sampling, it is necessary to provide topical anesthesia for patient comfort. The anesthetic of choice is 0.5% proparacaine because it causes the least bacterial growth inhibition. To enhance the bacterial yield, sterile preservative-free anesthetic may be used. Samples obtained may be inoculated directly onto soUd media plates (e.g., blood agar). Amies without charcoal transport medium (e g., BBL CultureSwab Plus) appears to be an acceptable alternative to direct plating and has the added benefit of convenience. 

U. Ambrose, K. Middleton and D. Seal. In vitro studies of water activity and bacterial growth inhibition of sucrose-polyethylene glycol 400-hydrogen peroxide and xylose-polyethylene glycol 400- hydrogen peroxide pastes used to treat infected wounds. Antimicrob. Agents Chemother., 35, 1799-803 (1991)... 

Nakai, S.A. and Siebert, K.J. 2003. Validation of bacterial growth inhibition models based on molecular properties of organic acids. International Journal of Food Microbiology 86 249-255. 

Although organic acids have been used for centuries to preserve foods, only recently has the mechanism by which bacterial growth inhibition... 

Sundman, V., and Sundman, J., Bacterial growth inhibiting effect of some phloroglucinol derivatives. Acta Pathol. Microbiol. Scand. 53, 345-355 (1961). 

Bacterial growth inhibition data have been reported for the -octyl and propargyl analogues (IV.3) and (IV.6) in comparison with MTX [63]. The IC50 values (IV.3) against S. faecium and L. casei were 0.72 and 0.030 nM. The corresponding values for (IV.6) were 0.41 and 0.027 nM, respectively, while those for MTX were 0.2 and 0.03 nM. Thus, the antibacterial activities of (IV.3) and (IV.6) were similar to MTX and were consistent with their anti-DHFR activity. Neither compound overcame MTX resistance in L. ca-sei/MTX. However, S. faeciumj Wn X was about 50-fold less resistant to (IV.3) than to MTX. 

The next significant advances were made by attempts to use substituent constants rather than physical measurements on the whole molecule. In 19 f0 Hammett (16) developed the (o ) substituent constants, which measure the degree of electron release/withdrawcil of aromatic substituents. Based on the Hammett equation, Hcinsen (17) correlated bacterial growth inhibition of a series of compounds with their Hammett [Pg.178]

An aqueous extract of senna leaf was tested in four different assays for genotoxic and mutagenic effects E. coli cultures, bacterial growth inhibition, reverse mutation test, and DNA strand break analysis in plasmid DNA. The extract produced single and double strand breaks in plasmid DNA in a cell-free system but was not cytotoxic or mutagenic in the E. coli strains tested (Silva et al. 2008). 

Encapsulation Entrapping of bacteria Bacterial growth inhibition... 

NBS is sharing with LC-MS/MS the same sample collection (filter paper collection and handling is now referred as dried blood spot (DBS)) and sometimes this creates some confusion in the terminology. For example, Guthrie card was intended to be the filter paper where blood is collected and today, with the adoption of LC-MS/MS, this definition is still used, even if LC-MS/MS has nothing to do with the Guthrie test (bacterial growth inhibition assay). 

Bacterial growth inhibition can be observed after 3 days of cultivation. If not, the incubation time needs to be extended to 4 days. 

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