Chromosomes bacterial artificial

A Bacterial Artificial Chromosome (BAC) is a vector that allows the propagation of larger exogenous DNA fragments, up to several hundred kb. BACs are propagated in recombination-deficient strains of E. coli. They are more stable and easier to handle than yeast artificial chromosomes (YACs). 

Nasmyth I think Tim Hunt s question is a sensible one. Normally p57 is expressed on the maternal chromosome and not on the paternal one, because according to this hypothesis the father s chromosome does not express it because he doesn t want to slow down growth. If this is the case, then if you had an extra copy and introduced it on a bacterial artificial chromosome (BAC) into the maternal genome, what would happen to growth This is a crucial prediction of the hypothesis. 

Suetsugu, Y., Minami, H., Shimomura, M. et al. 2007. End-sequencing and characterization of silkworm (Bombyx mori) bacterial artificial chromosome libraries. BMC Genomics, 8 314. 

Yamamoto, K., Narukawa, J., Kadono-Okuda, K. et al. 2006. Construction of a single nucleotide polymorphism linkage map for the silkworm, Bombyx mori, based on bacterial artificial chromosome end sequences. Genetics, 173(1) 151—161. 

Zeilhofer, H. U., Studler, B Arabadzisz, D. et al. (2005). Glycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome transgenic mice. J. Comp. Neurol. 482, 123-41. 

Kim U.J., Shizuya H., Deaven L., Chen X.N., Korenberg J.R., Simon M.I., Selection of a sublibrary enriched for a chromosome from total human bacterial artificial chromosome library using dna from flow-sorted chromosomes as hybridization probes. Nucl. Acids. Res. 1995 23 1838-1839. 

Gong, S., Zheng, C., Doughty, M. L., Losos, K., Hatten, M. E. and Heintz, N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425 917-925,2003. 

Bacterial ADPGPP enzymes, 12 491 Bacterial a-amylases, 10 280 Bacterial artificial chromosome (BAC) vectors, 12 508 Bacterial cellulose, 20 557 Bacterial genera, nitrogen fixation by, 17 295-296... 

Peptides with (K)ig domains can also form stable transfection particles with large DNA molecules including PI artificial chromosomes of 110 kb (28) and bacterial artificial chromosome (BAC) DNA constructs in a range of sizes up to 250 kb (29). The size, determined by atomic forces microscopy, of lipopolyplex particles formed with BAC DNA is directly proportional to the size of the BAC and transfections performed with equimolar amounts of 100-kb BACs and 8-kb pDNA are similar in efficiency. 

The principles that govern the delivery of recombinant DNA in clonable form to a host cell, and its subsequent amplification in the host, are well illustrated by considering three popular cloning vectors commonly used in experiments with E. coli—plasmids, bacteriophages, and bacterial artificial chromosomes—and a vector used to clone large DNA segments in yeast. 

Cloning vectors include plasmids, bacteriophages, and, for the longest DNA inserts, bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs). 

Studies of overall genome composition based on reassociation kinetics (Simpson et ai, 1982 Cox et ai, 1990 Marx et a/., 2000) and analysis of fully sequenced bacterial artificial chromosome (BAC) clones from the 5. mansoni genome project show that platyhelminth genomes contain abundant highly and moderately repetitive sequence (Fig. 2.1). Much of the repetitive DNA comprises two classes of integrated mobile elements class I elements, which include long terminal repeat (LTR) retrotransposons and retroviruses, non-LTR retro-transposons and short interspersed nuclear elements (SINES) and transpose via an RNA intermediate, and class II elements (trans-posons), which transpose as DNA (Brindley et ai, 2003). Additionally, small dispersed or tandemly repeated sequences are common. A wide variety of these sequences have been isolated and characterized from a variety of taxa (Table 2.4). 

---