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Recombinant bacterial artificial chromosomes

A Bacterial Artificial Chromosome (BAC) is a vector that allows the propagation of larger exogenous DNA fragments, up to several hundred kb. BACs are propagated in recombination-deficient strains of E. coli. They are more stable and easier to handle than yeast artificial chromosomes (YACs). [Pg.245]

The principles that govern the delivery of recombinant DNA in clonable form to a host cell, and its subsequent amplification in the host, are well illustrated by considering three popular cloning vectors commonly used in experiments with E. coli—plasmids, bacteriophages, and bacterial artificial chromosomes—and a vector used to clone large DNA segments in yeast. [Pg.311]

Messerle M, Hahn G, Brune W, Koszinowski UH (2000) Cytomegalovirus bacterial artificial chromosomes a new herpesvirus vector approach. Adv Virus Res 55 463 -478 Mocarski-ES J, Kemble GW (1996) Recombinant cytomegaloviruses for study of replication and pathogenesis. Intervirology 39 320 330... [Pg.21]

Key words Recombineering, Hit and Fix method. Oligonucleotide, Bacterial artificial chromosome, Point mutation... [Pg.111]

Muyrers JP, Zhang Y, Testa G, Stewart AF (1999) Rapid modification of bacterial artificial chromosomes by ET-recombination. Nucleic Acids Res 27 1555-7. [Pg.131]

Various bacterial plasmids, bacteriophages, and yeast artificial chromosomes are used as cloning vectors (Brown, 2001). At the heart of the general approach to generating and propagating a recombinant DNA molecule is a set of enzymes which synthesize, modify, cut, and join DNA molecules. [Pg.169]

A major advance in replication-defective adenoviruses is the development of methods for creating recombinant viruses from distinct molecular clones in which the entire genome of the vector has been subcloned into a prokaryotic or invertebrate host. This has now been accomplished in a number of systems including yeast artificial chromosomes (21), cosmids (22), and bacterial plasmids (23-25). The subsequent discussion will focus on methods in which the adenoviral genome is subeloned in its entirety into bacterial plasmids,... [Pg.36]


See other pages where Recombinant bacterial artificial chromosomes is mentioned: [Pg.1235]    [Pg.277]    [Pg.313]    [Pg.1497]    [Pg.1235]    [Pg.215]    [Pg.714]    [Pg.714]    [Pg.634]    [Pg.83]    [Pg.432]    [Pg.313]    [Pg.584]    [Pg.563]    [Pg.122]    [Pg.111]    [Pg.112]    [Pg.122]    [Pg.134]    [Pg.9]    [Pg.855]    [Pg.140]    [Pg.195]   
See also in sourсe #XX -- [ Pg.145 ]




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Bacterial artificial chromosome

Chromosomal recombination

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