Bacterial artificial chromosome vector

Frengen E, Zhao B, Howe S, et al. (2000). Modular bacterial artificial chromosome vectors for transfer of large inserts into mammalian cells. Genomics. 68 118-126. 

A Bacterial Artificial Chromosome (BAC) is a vector that allows the propagation of larger exogenous DNA fragments, up to several hundred kb. BACs are propagated in recombination-deficient strains of E. coli. They are more stable and easier to handle than yeast artificial chromosomes (YACs). 

Bacterial ADPGPP enzymes, 12 491 Bacterial a-amylases, 10 280 Bacterial artificial chromosome (BAC) vectors, 12 508 Bacterial cellulose, 20 557 Bacterial genera, nitrogen fixation by, 17 295-296... 

The principles that govern the delivery of recombinant DNA in clonable form to a host cell, and its subsequent amplification in the host, are well illustrated by considering three popular cloning vectors commonly used in experiments with E. coli—plasmids, bacteriophages, and bacterial artificial chromosomes—and a vector used to clone large DNA segments in yeast. 

Cloning vectors include plasmids, bacteriophages, and, for the longest DNA inserts, bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs). 

DNA amplified as bacterial artificial chromosome in Escherichia coli Rescue of replication-competent virus progeny and packaging of amplicon vectors. Hum Gene Ther 9 2787-2794. 

Saeki Y, Fraefel C, Ichikawa T, Breakefield XO, Chiocca EA (2001) Improved helper virus-free packaging system for HSV amplicon vectors using an ICP27-deleted, oversized HSV-1 DNA in a bacterial artificial chromosome. Mol Ther 3 591-601. 

Saeki Y, Ichikawa T, Saeki A, Chiocca EA, Tobler K, et al. 1998. Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coir. rescue of replication-competent virus progeny and packaging of amplicon vectors. Hum. Gene Ther. 9 2787-94... 

Messerle M, Hahn G, Brune W, Koszinowski UH (2000) Cytomegalovirus bacterial artificial chromosomes a new herpesvirus vector approach. Adv Virus Res 55 463 -478 Mocarski-ES J, Kemble GW (1996) Recombinant cytomegaloviruses for study of replication and pathogenesis. Intervirology 39 320 330... 

Various bacterial plasmids, bacteriophages, and yeast artificial chromosomes are used as cloning vectors (Brown, 2001). At the heart of the general approach to generating and propagating a recombinant DNA molecule is a set of enzymes which synthesize, modify, cut, and join DNA molecules. 

Various bacterial plasmids, bacteriophages and yeast artificial chromosomes are used as cloning vectors (Brown, 2(X)1), such as pBR322. 

A major advance in replication-defective adenoviruses is the development of methods for creating recombinant viruses from distinct molecular clones in which the entire genome of the vector has been subcloned into a prokaryotic or invertebrate host. This has now been accomplished in a number of systems including yeast artificial chromosomes (21), cosmids (22), and bacterial plasmids (23-25). The subsequent discussion will focus on methods in which the adenoviral genome is subeloned in its entirety into bacterial plasmids,... 

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