Artificial chromosomes yeast

Eig. 5. Restriction map of the yeast artificial chromosome (YAC) vector used for cloning very large fragments of eukaryotic DNA. Terms defined in text... 

A Bacterial Artificial Chromosome (BAC) is a vector that allows the propagation of larger exogenous DNA fragments, up to several hundred kb. BACs are propagated in recombination-deficient strains of E. coli. They are more stable and easier to handle than yeast artificial chromosomes (YACs). 

An yeast artificial chromosome (YAC) is a vector that allows the propagation of large exogenous DNA fragments, up to several megabases, in yeast. 

Yeast artificial chromosome (YAC) Large segments of DNA (up to 1 million bases) from another species spliced into DNA of yeast. The new construct that carries the foreign DNA is called a vector. 

Yeast artificial chromosome (YAC) cloning experiments, 22 507-508 Yeast biomass... 

Amyloid production and deposition are increased in AD patients with PS mutations, in transgenic mice with PS mntations, and in mntant APP and PSl yeast artificial chromosome transgenic mice (166-169). Transgenic mice with AD-related PSl mntations show accelerated nenrodegeneration with intracellular ABP deposition and withont amyloid plaqne formation, snggesting that PSl mutation is npstream of the amyloid cascade in AD (170). 

Lamb, B.T., Bardel, K.A., Kulnane, L.S., et al. (1999) Amyloid production and deposition in mutant amyloid precursor protein and presenUin-1 yeast artificial chromosome transgenic mice. Nat. Neurosci., 2, 695-697. 

The immunoglobulin k locus of the mouse in comparison to the human k locus Use of yeast artificial chromosomes (YACs) for transgenesis... 

Research work with large genomes and the associated need for high-capacity cloning vectors led to the development of yeast artificial chromosomes (YACS Fig. 9-8). YAC vectors contain all the elements needed to maintain a eukaryotic chromosome in the yeast nucleus a yeast origin of replication, two selectable markers, and specialized sequences (derived from the telomeres and centromere, regions of the chromosome discussed in Chapter 24) needed for stability and... 

Cloning vectors include plasmids, bacteriophages, and, for the longest DNA inserts, bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs). 

Artificial chromosomes (Chapter 9) have been constructed as a means of better understanding the functional significance of many structural features of eukaryotic chromosomes. A reasonably stable artificial linear chromosome requires only three components a centromere, telomeres at each end, and sequences that allow the initiation of DNA replication. Yeast artificial chromosomes (YACs see Fig. 9-8) have been developed as a research tool in biotechnology. Similarly, human artificial chromosomes (HACs) are being developed for the treatment of genetic diseases by somatic gene therapy. 

Yeast Artificial Chromosomes (YACs) YACs are used to clone large pieces of DNA in yeast cells. What three types of DNA sequences are required to ensure proper replication and propagation of a YAC in a yeast cell ... 

For repetitive DNA sequences, such as alphoid repeats, especially for identification of centromeric regions on chromosomes, as well as in mterphase nuclei, I routinely prepare labeled probes by PCR using a centromeric DNA-specific set of primers. For single-copy gene mapping by FISH, it is essential to use DNA cloned into either cosmids or in yeast artificial chromosome vectors (YAC) m order to obtain the level of sensitivity that will enable visualization of signal with a fluorescence microscope... 

Maynard Olson and colleagues at Washington University invented yeast artificial chromosomes, or YACs, expression vectors for large proteins. 

Yeast artificial chromosomes have been developed that can clone hundreds of kilobases of DNA (see Watson, Gilman, Wilkowski, and Zoller reference). 

Tanaka, M., Hirai, H., LoVerde, P.T., Nagafuchi, S., Franco, C.R., Simpson, A.J. and Pena, S.D. (1995) Yeast artificial chromosome (YAC)-based genome mapping of Schistosoma mansoni. Molecular and Biochemical Parasitology 69, 41-51. 

Brensing-Kiippers, J Zocher, I., Thiebe, R., Zachau, H.G. (1997). The human immunoglobulin kappa locus on yeast artificial chromosomes (YACs). Gene 191,173-181. 

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