Chromosome sorting

Ibrahim SF, Engh van den G. 2004. High-speed chromosome sorting. Chromosome Res 12 5-14. 

Hashimoto, K. (1992) Flow karyotyping and chromosome sorting. Nippon Rinsho. 50,2484-2488. 

For discussion of chromosome sorting by optical zapping, see Roslaniec MC, Reynolds RJ, Martin JC, et al. (1996). Advances in flow cytogenetics Progress in the development of a high speed optical chromosome sorter based on photochemical adduct formation between psoralens and chromosomal DNA. NATO Advanced Studies Series, Flow and Image Cytometry 95 104-114. 

Fig. 11.13. DNA blot analysis using spots from chromosomes sorted directly onto filter paper on the basis of their Hoechst 33258 fluorescence. From Van Dilla et al. (1990).

Fig. 8.20. Reprinted with permission of John Wiley Sons, Inc. 1990 from Gray JW and Cram LS (1990). Flow karyotyping and chromosome sorting. Melamed MR, et al. (eds). Flow Cytometry and Sorting. New York Wiley-Liss, pp 503-529. The work was performed at the University of California Lawrence Livermore National Laboratory under the auspices of the U.S. Department of Energy.

Ehrlich, G., Viegas-Pequignot, E., Ginzberg, D., Sindel, L., Soreq, H., Zakut, H. (1992). Mapping the human acetylcholinesterase gene to chromosome 7q22 by fluorescent in situ hybridization coupled with selective PCR amplification from a somatic hybrid cell panel and chromosome-sorted DNA libraries. Genomics 13 1192-7. 

Gray JW, Cram LS (1990) Flow karyotyping and chromosome sorting. In Melamed MR, Lindmo T, Mendelsohn M (eds) Flow cytometry and sorting. Wiley-Liss, New York, pp 503-530... 

In the analysis of genomes, it is often useful to begin with a less complex mixture than an entire genome DNA sample. Individual chromosomes can be obtained in high purity using a technology known as chromosome sorting [8], a form of flow cytometry. A suspension of chromosomes stained with fluorescent... 

Kim U.J., Shizuya H., Deaven L., Chen X.N., Korenberg J.R., Simon M.I., Selection of a sublibrary enriched for a chromosome from total human bacterial artificial chromosome library using dna from flow-sorted chromosomes as hybridization probes. Nucl. Acids. Res. 1995 23 1838-1839. 

Boschman, G. A., Buys, C. H., van der Veen, A. Y Rens, W., Osinga, J., Slater, R. M and Aten, J. A. (1993) Identification of a tumor marker chromosome by flow sorting, DNA amplification in vitro, and in situ hybridization of the amplified product. Genes Chromosom. Cancer 6, 10-16. 

Termination Eventually, the two replication forks of the circular E. coli chromosome meet at a terminus region containing multiple copies of a 20 bp sequence called Ter (for terminus) (Fig. 25-17a). The Ter sequences are arranged on the chromosome to create a sort of trap that a replication fork can enter but cannot leave. The Ter sequences function as binding sites for a protein called Tus (terminus utilization substance). The Tus-Ter complex can arrest a replication fork from only one direction. Only one Tus-Ter complex functions per replication cycle—the complex first encountered by either... 

One way to select a desired segment of DNA from a digest of chromosomal DNA is to sort out the "restriction fragments" by gel electrophoresis.613 The DNA from the gel can be transferred to a nitrocellulose sheet while retaining the separation pattern using a method devised by Southern.560 614 615 In this Southern blot technique, solvent flows from a pool beneath the gel up through the gel and the nitrocellulose sheet into paper towels. The DNA is trapped on the nitrocellulose in the same pattern observed in the electrophero-gram. A suitably labeled probe such as cDNA with... 

Microtubules, especially those that make up the mitotic spindle, are in a delicate state of balance between assembly and disassembly. This is because both the formation of the spindle and the movement of chromosomes to opposite spindle poles depend on carefully coordinated extension or shrinkage at both ends of the microtubules in the spindle. The end of a microtubule that terminates with /1-tubulin is more dynamic than the other end, which has an a-tubulin monomer as its final subunit. In cells, microtubules usually grow out from some sort of organizing... 

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