Alkaline phosphatase staining

Fig. 14 Alkaline phosphatase staining of recovered ES cells after dissociation of PMBV/PVA hydrogel (left), and of the ES cells cultured on PMB30 (right). The undifferentiated ES cells were well stained. ES cells encapsulated in the PMBV/PVA hydrogel maintained their undifferentiated character during the 3 days of encapsulation...

Background seen in all control and specimen tissue when using an alkaline phosphatase staining system. ... 

Van der Loos CM, Becker AE (1994) Double epi-illumination microscopy with separate visualization of two antigens a combination of epi-polarization for immunogold-silver staining and epi-fluorescence for alkaline phosphatase staining. J Histochem Cytochem 42 289-295... 

Measurement of serum iron, determination of peroxidase and of alkaline phosphatase activity In peripheral granulocytes can be performed in most pathology laboratories. Peroxidase and alkaline phosphatase staining are usually undertaken when the index of suspicion for leukemia is high. 

Figure 3 Immuno localization of acetyl esterase. Sections were incubated with antibodies raised against the acetyl esterase, followed by visualization with alkaline phosphatase conjugated secondary antibodies and staining with Fast Red.

Van der Loos CM, Teeling P. A generally applicable sequential alkaline phosphatase immunohistochemical double staining. I. Histotechnol. 2008 31 119-127. 

The PMBV/PVA hydrogel containing ES cells was dissociated by the addition of 0.2 M D-fructose solution after 3 days. After dissociation of the PMBV/PVA hydrogel, the recovered ES cells were cultured on gelatin-coated TCPS in the culture medium as usual, and the differentiation characters of recovered ES cells were estimated by alkaline phosphatase (ALP) staining (Fig. 14). 

Introduction The Primary Antibodies Visualization of the Target/Staining Methods Enhancing of Signals with Antibody Complexes A Protocol for Immunovisualization of Proteins with Alkaline Phosphatase Conclusions References... 

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

When using an enzyme (peroxidase or alkaline phosphatase) marker, incubate sections in an enzyme substrate solution until desired stain intensity develops (see Sect. 2.3). A fluorophore label can be directly visualized in a fluorescent microscope. 

The on-bead assay was conducted according to Scheme 3.19, which shows the chain of events, which leads to a colorimetric response, when an oligosaccharide binds effectively to the B. purpurea lectin. The lectin was covalently linked to biotin, a small molecule with an extremely high affinity for streptavidin. The bead-lectin-biotin conjugates were then exposed to streptavidin, linked to the enzyme alkaline phosphatase. Alkaline phosphatase hydrolyses phosphate esters [e.g., 5-bromo-4-chloro-3-indolyl phosphate (BCIP), 110]. When the 5-bromo-4-chloro-3-hydroxyindole (111) is released, in the presence of nitro blue tetrazolium (NBT), it forms a dark purple, insoluble dye, thus staining beads where there was a favorable binding interaction. 

Golgi, in early neuroanatomical studies (1898) staining neurones by silver impregnation, observed a reticular apparatus which was crescent shaped and appeared to be linked through canaliculi. The structure was also seen in secretory cells. Between 1949 and 1954, Baker reported the presence of similar systems in unfixed cells examined by phase contrast. The structures could be stained by osmium tetroxide and probably contained lipid. They also stained for glycoprotein and alkaline phosphatase. Baker s confirmation of the existence of the... 

The acoustic micrograph in Fig. 1.5(a) came from a 5-week-old preparation. It was fixed in alcohol, and stained for alkaline phosphatase and, with von Kossa stain, for biomineral material. The biomineral material of interest here is hydroxyapatite, the principal crystalline mineral constituent of bone. The ordered structure visible within the matrix is not seen with either the light or electron microscopes. But the acoustic microscope can also work perfectly well with unfixed, unstained specimens. Figure 1.5(b) is an acoustic micrograph of matrix and cells from a 17-year-old male. In addition to the standard ingredients of culture medium, these cells were specifically stimulated with beta-glycerolphosphate and a vitamin C preparation. Because the acoustic... 

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