Gelatinous coating

Reagent layer 1 porous gelatin coating film of urease and pH buffer... 

Encapsulated scale inhibitors have been developed. This type of scale inhibitor allows chemical release over an extended period [865,1452], Microencapsulated formulations may contain a gelatin coating with a multipurpose cocktail, such as [1006,1007] ... 

NOTE Brain sections are at the level of bregma -3.8 mm of the rat brain atlas (paxinos and Watson 1982). 20-Micron thick coronal sections of frozen rat brain were cut, thaw-mounted onto gelatin-coated slides, and incubated with (a) 10 nM 3H-PCP... 

T Dahl, ILT Sue, A Yum. The effect of pancreatin on the dissolution performance of gelatin-coated tablets exposed to high-humidity conditions. Pharm Res 8 412-414,1991. 

M Aikman, L Augsburger, I Berry et al. Collaborative development of two-tiered dissolution testing for gelatin capsules and gelatin-coated tablets using enzyme-containing media. Pharmacop Forum 24(5) 7045-7050, 1998. 

Fig. 20.2 Chronic lithium and valproate robustly increase bcl-2 immunoreactive neurons in the frontal cortex. Male Wistar Kyoto rats were treated with either Li2C03, valproate or saline by twice daily i.p. injections for four weeks. Rats brains were cut at 30 pm serial sections were cut coronally through the anterior portion of the brain, mounted on gelatin-coated glass slides and were stained with thio-nin. The sections of the second and third sets were incubated free-floating for 3 d at 4°C in 0.01 M PBS containing a polyclonal antibody against bcl-2 (N-19, Santa Cruz Biotechnology,...

The PMBV/PVA hydrogel containing ES cells was dissociated by the addition of 0.2 M D-fructose solution after 3 days. After dissociation of the PMBV/PVA hydrogel, the recovered ES cells were cultured on gelatin-coated TCPS in the culture medium as usual, and the differentiation characters of recovered ES cells were estimated by alkaline phosphatase (ALP) staining (Fig. 14). 

Tan, K. M. Tay, C. M. Tjin, S. C. Chan, C. C. Rahardjo H., High relative humidity measurements using gelatin coated long period grating sensors, Sens. Actuators B 2005, 110, 335 341... 

Dichromated gelatin coatings, 12 444 Dichromated resists, 15 156 Dichromate treatment, 9 397 Dickite, 6 659... 

After the completion of the in vivo microdialysis experiment, rats are euthanized with an overdose of sodium pentobarbital (100 mg/kg) and perfused intracardially with 0.9% saline followed by 10% formalin. The brains are extracted and stored in a 10% formalin solution until they are sectioned into slices (40-60 mm) consecutively through the guide cannula tract. The sections are mounted onto gelatin-coated glass slides and stained with thionin. An observer unaware of the rat s treatment or results verifies the cannula location for each rat. 

Current Trends in Dissolution—Implications for HPLC Methods I. Gelatin Coatings... 

A recent addition to General Chapter Dissolution <711> allows for the addition of enzyme to the dissolution medium. This is justified when gelatin-coated tablets or gelatin-encapsulated products exhibit a slow-down in the dissolution rate attributed to the crosslinking of the gelatin. The addition of enzyme will break up the crosslinking and therefore remove the... 

APES may interfere with silver salt solutions in intensification steps of immunogold silver-staining techniques therefore, for those applications, poly-L-lysine or gelatin-coated slides are preferable. 

Efficiency of stably integrated shRNA vectors should be confirmed in vitro. Therefore, expand two positives clones per shRNA construct on feeders, freeze aliquots, and cultivate the cells feeder-free on gelatine-coated 10 cm dishes for at least 2 days. Harvest cells and analyze the four clones for knockdown efficiency as described in steps 2 and 3 of Subheading 3.1.2. Determine the best performing clone for each siRNA target sequence. 

A modified version of the ECVAM-approved embryonic stem cell test (EST) (52) was recently developed by Hunter and coworkers in NHEERL (31). This assay is capable of quantitatively assessing cytotoxicity and cardiomyocyte differentiation and was used to test the ToxCast Phase I chemical library (32). Briefly, male murine J1 mES cells were seeded onto gelatin-coated 96-well plates at a known density in differentiation media on day 0. On day 1, cells were treated with chemicals ranging from 0.0125 to 12.5 pM, and on day 9 In-Cell Western (Li-Cor Biosciences) assays were assayed for a- and P-cardiac Myosin Heavy Chain (MYH6/MYH7)... 

Dissolution testing should be carried out in USP Apparams 1 at 100 rpm or Apparatus 11 at 50 rpm using 900 ml of the following dissolution media (1) 0.1 N HCl or Simulated Gastric Fluid USP without enzymes (2) a pH 4.5 buffer and (3) a pH 6.8 buffer or Simulated Intestinal Fluid USP without enzymes. For capsules and tablets with gelatin coating. Simulated Gastric and Intestinal Fluids USP (with enzymes) can be used. 

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