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Albumin, serum chemical binding

Protein concentration can be determined using a method introduced by Bradford,4 which utilises Pierce reagent 23200 (Piece Chemical Company, Rockford, IL, USA) in combination with an acidic Coomassie Brilliant Blue G-250 solution to absorb at 595 nm when the reagent binds to the protein. A 20 mg/1 bovine serum albumin (Piece Chemical Company, Rockford, IL, USA) solution will be used to prepare a standard calibration curve for determination of protein concentration. The sample for analysis of SCP is initially homogenised or vibrated in a sonic system to break down the cell walls. [Pg.16]

A variety of hydrolases catalyze the hydrolysis of acetylsalicylic acid. In humans, high activities have been seen with membrane-bound and cytosolic carboxylesterases (EC 3.1.1.1), plasma cholinesterase (EC 3.1.1.8), and red blood cell arylesterases (EC 3.1.1.2), whereas nonenzymatic hydrolysis appears to contribute to a small percentage of the total salicylic acid formed [76a] [82], A solution of serum albumin also displayed weak hydrolytic activity toward the drug, but, under the conditions of the study, binding to serum albumin decreased chemical hydrolysis at 37° and pH 7.4 from tm 12 1 h when unbound to 27 3 h for the fully bound drug [83], In contrast, binding to serum albumin increased by >50% the rate of carboxylesterase-catalyzed hydrolysis, as seen in buffers containing the hydrolase with or without albumin. It has been postulated that either bound acetylsalicylic acid is more susceptible to enzyme hydrolysis, or the protein directly activates the enzyme. [Pg.405]

Mir MM, Fazili KM, Abul Qasim M. Chemical modification of buried lysine residues of bovine serum albumin and its influence on protein conformation and bilirubin binding. Biochim. Biophys. Acta 1992 1119 261-267. [Pg.321]

Chemical reactions Polymerization of casein and whey proteins are due to some kind of chemical reactions. The different proteins as found in the supernatant of milk after precipitation at pH 4.6 are collectively called whey proteins. These globular proteins are more water soluble than caseins and are subject to heat dena-turation. Denaturation increases their water-binding capacity. The principal fractions are P-lactoglobulin, a-lactalbumin, bovine serum albumin (BSA), and immunoglobulins (Ig). [Pg.208]

Fig. 2 Determination of the binding stoichiometry of human serum albumin (HSA) to its mouse monoclonal IgG antibody (anti-HSA). (A) The concentration of anti-HSA was 0.33 /J.M. DNS-E was dansylglutamic acid used as internal standard. The intermediate species is considered to be due to the 1 1 complex. (B) A plot of the concentration of free ligand vs. the ratio of [HSA]/[anti-HSA] gives a sharp break at the stoichiometric point. (Reprinted with permission from Ref. 8. Copyright 1994 American Chemical Society.)... Fig. 2 Determination of the binding stoichiometry of human serum albumin (HSA) to its mouse monoclonal IgG antibody (anti-HSA). (A) The concentration of anti-HSA was 0.33 /J.M. DNS-E was dansylglutamic acid used as internal standard. The intermediate species is considered to be due to the 1 1 complex. (B) A plot of the concentration of free ligand vs. the ratio of [HSA]/[anti-HSA] gives a sharp break at the stoichiometric point. (Reprinted with permission from Ref. 8. Copyright 1994 American Chemical Society.)...
Damsten, M.C., Commandeur, J.N.M., Fidder, A., Hulst, A.G.,Touw, D., Noort, D. and Vermeulen, N.P.E. (2007) Liquid chromatography/tandem mass spectrometry detection of covalent binding of acetaminophen to human serum albumin. Drug Metabolism and Disposition The Biological Fate of Chemicals, 35 (8), 1408-1417. [Pg.163]

For the modification of the gold surface different chemical and biological substances (dodecanethiol, lectins, dextran sulfate DS, with the mass of 5 kDa and polyelectrolytes) were used. The mercaptane layer was formed in ethanol atlO" M concentration of thiol. Polyaniline hydrochloride (PAH), Aldrich , USA, was used to cover the transducer surface by a water insoluble polymer. Lectins from Phaseolus vulgaris (PLA), Solanum tuberosum (STA), Helix pomatia (HPA) and Tuberosum vulgaris (WGA) were immobilized on the surface pre-treated with dodecanethiol or PAH. These lectins were used for the immobilization of glycoproteins. Blocking of the free binding sites was carried out with 1% bovine serum albumin (BSA). [Pg.79]

Reactions between cisplatin and serum albumin are thought to be the main route for platinum binding in human blood plasma. Several clinical and experimental observations have suggested that albumin-bound platinum may be anticancer active [47] [48]. Additionally, albumin binding may reduce some of the side-effects of cisplatin treatment, especially its nephrotoxicity [49]. The reaction of cisplatin with intact and chemically modified recombinant human albumin (rHA), and with HSA (human serum al-... [Pg.315]

The available concentration of a compound may determine whether it is toxic to cells. The concentration of a test compound available to cells may not be the same as the final concentration added to the sample if the toxin is bound by albumin present in serum used to supplement the medium. Chemical compounds also may bind to plastic surfaces, become concentrated inside cells, be chemically converted by modifying enzymes in the cytoplasm, or actively pumped out of cells. [Pg.104]

The separation technique can be used in two different ways for the measurements of physico-chemical parameters. In one approach the propenies of the compounds are characterised directly from the chromatographic retention which is determined by the interaction of solutes with the stationary and the mobile phases. This approach can be used for lipophilicity determination, measurements of serum albumin binding and estimating the membrane transport of compounds from their retention on immobilised artificial membrane. [Pg.545]


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See also in sourсe #XX -- [ Pg.388 , Pg.463 , Pg.466 , Pg.471 , Pg.472 ]




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