Bilirubin binding

Mir MM, Fazili KM, Abul Qasim M. Chemical modification of buried lysine residues of bovine serum albumin and its influence on protein conformation and bilirubin binding. Biochim. Biophys. Acta 1992 1119 261-267. 

FlC. 9. Nonparallel regeneration of the functions for bilirubin binding and fatty acid binding in bovine plasma albumin. The oxidative regeneration of reduced albumin was carried out at protein concentrations of 1 ft,M at 25°C in 0.10 M Tris-chloride buffer, pH 8.0, containing 1 mM EDTA and 1 mM reduced and 0.10 mM oxidized glutathione (Johanson et al, 1977, 1981). 

M8. Meuwissen, J. A. T. P., Fevery, J., and Heirwegh, K. P. M., Bilirubin-binding properties of plasma and liver cytosol preparations from rats. Biochem. J. 126, 8P (1971). 

M9. Meuwissen, J., Heirwegh, K., and De Groote, J., Intracellular bilirubin binding to proteins. World Congr. Gastroenterol. [Proc.], 3rd, Tokyo, 1966 4, 85-86 (1967). 

A) Competes for the bilirubin-binding sites on plasma proteins... 

J. Jacobsen and R, Broderson, Albumin-bilirubin binding mechanism Kinetic and spectroscopic studies of binding of bilirubin and xanthobilirubic acid to human serum albumin, J. Biol. Chem., 258 6319 (1983). 

Concern has been expressed over the use of methylparaben in infant parenteral products because bilirubin binding may be affected, which is potentially hazardous in hyperbilirubinemic neonates. ... 

W5. Watson, D., Bilirubin-binding capacity of blood plasma in relation to foetal erythroblastosis. Australia and New Zealand J. Obstel. Gynaecol. 6, 121-124 (1964). 

H45. Hirano, K., Watanabe, Y., Adachi, T., and Sugiura, M., Carrier proteins in human fetal serum Bilirubin-binding abilities of albumin, alpha-fetoprotein and ligandin. Chem. Pharm. Bull. 32, 708-715 (1984). 

Twenty percent of all human newborns accumulate enough bilirubin to stain their skin, resulting in jaundice. Bilirubin binds to cellular and mitochondrial membranes, causing cell death in a variety of tissues clinically, bilirubin toxicity may lead to mental retardation, cerebral palsy, deafness, seizures, or death. 

In addition, there are some hepatic metabolic uses. Because hepatic glucuronyl transferase and the bilirubin-binding Y protein are increased by the barbiturates, phenobarbital has been used successfully to treat hyperbilirubinemia and kernicterus in the neonate. The nondepressant barbiturate phetharbital (A-phenylbarbital) works equally well. Phenobarbital may improve the hepatic transport of bihrubin in patients with hemolytic jaundice. 

L. Soltes, B. Sebille, J. P. Tillement and D. Berek, Study of bilirubin binding in human serum by high-performance liquid chromatography, /. Clin. Chem. Clin. Biochem., 1989, 27, 935-939. 

Jacobsen, C. Chemical Modification of the High-Affinity Bilirubin Binding Site of Human Serum Albumin. Eur. J. Biochem. 27, 513-519 (1972). 

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