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Agrobacterium transformation system

Figure 13. Agrobacterium tumefaciens, the natural transformation system for plants. The bacterium contains a circular piece of DNA, the Ti-plasmid. The bacterium infects a dicotyledonous plant and transfers the T-DNA from the Ti-plasmid to the plant chromosomal DNA causing a tumor to form. The tumor is called a "crown gall". The gall, or teratoma, can be removed from the plant and placed into culture on medium without exogenous hormones. Figure 13. Agrobacterium tumefaciens, the natural transformation system for plants. The bacterium contains a circular piece of DNA, the Ti-plasmid. The bacterium infects a dicotyledonous plant and transfers the T-DNA from the Ti-plasmid to the plant chromosomal DNA causing a tumor to form. The tumor is called a "crown gall". The gall, or teratoma, can be removed from the plant and placed into culture on medium without exogenous hormones.
F, et al (2012) Development of efficient Catharanthus roseus regeneration and transformation system using Agrobacterium tumefaciens and hypocotyls as explants, BMC Biotechnol, 12, 34. [Pg.265]

In order to reduce the time required to confirm the accumulation of a given recombinant protein, we have developed a cell culture system in which transgenic alfalfa callus material produced at the proliferation step of Agrobacterium-based transformation is used to initiate cell cultures. These cell suspensions can be subcultured to sustain batch production of modest protein amounts. The protein blot shown in Fig. 1.2 demonstrates our ability to detect a recombinant protein in total... [Pg.6]

Agrobacterium tumefaciens transformation, 22 491 system, 22 488 Agro-based fibers, 22 17-18 Agrochemical market, profitability in, 23 285... [Pg.23]

Fuentes, A. Ramos, P.L. Ayra, C. Rodriguez, M. Ramirez, N. Pujol, M. Development of a highly efficient system for assessing recombinant gene expression in plant cell suspensions via Agrobacterium tumefaciens transformation. Biotechnol. Appl. Biochem. 2004, 39, 355-361. [Pg.2499]

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See also in sourсe #XX -- [ Pg.351 ]

See also in sourсe #XX -- [ Pg.351 ]



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