High-performance affinity

Rush, R. S., Derby, P. L., Strickland, T. W., and Rohde, M. F. (1993). Peptide-mapping and evaluation of glycopeptide microheterogeneity derived from endoproteinase digestion of erythropoietin by affinity high-performance capillary electrophoresis. Anal. Chem. 65, 1834—1842. 

Solid Adsorption Ion exchange Exclusion Affinity High-performance thin-layer chromatography (HPTLC)... 

J. B. Wheatley, J. A. Montali and D. E. Schmidt-Jr, Coupled affinity-reversed-phase high-performance liquid cliromatography systems for the measurement of glutathione 5-transferases in human tissues , 7. Chromatogr. A 676 65 - 79 (1994). 

B. Nilsson, Exti action and quantitation of cortisol by use of high-performance liquid affinity cliromatogr aphy , J. Chromatogr. 276 413-417 (1983). 

Lipophilicity represents the affinity of a molecule or a moiety for a lipophilic environment. It is commonly measured by its distribution behavior in a biphasic system, either liquid-liquid (e.g. partition coefficient in 1-octanol-water) or solid-liquid (retention on reversed-phase high-performance liquid chromatography or thin-layer chromatography system). 

Baker et al. [137] reported that Rhesus monkeys were administered primaquine (6-10.5 mg as the phosphate/kg intravenously) and plasma samples were analyzed by high performance liquid chromatography for the presence of the unchanged drug and the major metabolite, 8-[3-carboxy-l-methylpropylamino)-6-methoxyquinoline. Primaquine had an unusually high affinity for tissue compartments, which produced a rapid initial drop in plasma concentration. Within 15 min, the plasma concentration of the metabolite far exceeded that of primaquine. Thirty-five to eighty-three percent of the primaquine dose was converted to the major metabolite. This metabolite possessed much lower affinity for the tissues compartments than the drug itself. 

High performance adhesives, 1 545 High performance affinity chromatography support, 6 394-395... 

A third area of development in carbohydrate l.c. analyses is in the combined techniques (see Section IV,3) and other methods that provide qualitative, as well as quantitative, information about sample constituents, such as high-performance liquid affinity chromatography. The use of specific lectin- and monoclonal antibody-based, stationary phases for analytical and preparative applications is now being considered. The basic concepts of these techniques have been reviewed - and their applications to carbohydrates have been discussed. 

The issue of which antibody to select for an assay is not a new problem. Certainly anyone involved in the development of an immunoassay has been faced with this choice. Consider attempting to create a multianalyte, microarray-based micro-ELISA of modest density (10 to 100 analytes) and determining which capture antibodies to use based upon their affinities, stabilities, and cross-reactivities. For a sandwich assay, add in the 10 to 100 analyte-specific secondary (reporter) antibodies and determine their levels of cross-reactivity with each other and with the specified antigens and capture antibodies. In other words, achieving high performance for all analytes with a microarray immunoassay is indeed a formidable challenge. 

Shibukawa et al. (40) discussed the frontal analysis method, also called high-performance frontal analysis (HPFA) or high-performance capillary electrophoresis/frontal analysis (HPCE/FA), compared it to conventional methods, and focused on the application to stereoselective protein binding. The affinity of the drugs warfarin, verapamil, and carbamazepine and the drug candidate BOF-4272 to HSA was investigated. 

Y Kuroda, B Cao, A Shibukawa, T Nakagawa. Effect of oxidation of low-density lipoprotein on drug binding affinity studied by high-performance frontal analysis—capillary electrophoresis. Electrophoresis 22 3401—3407, 2001. 

A Ahmed, H Ibrahim, F Pastore, DK Lloyd. Relationship between retention and effective selector concentration in affinity capillary electrophoresis and high-performance liquid chromatography. Anal Chem 68 3270—3273, 1996. 

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