High performance liquid affinity chromatography HPLAC

Ohlson, S., Nilsson, R., Niss, U., Kjellberg, B. M., and Freiburghaus, C. (1988) A novel approach to monoclonal antibody separation using high performance liquid affinity chromatography (HPLAC) with SelectiSpher-10 protein G. J. Immunol. Methods 114,175-180... 

Heparinlike sulfated dextran derivatives, like carboxymethyldextran benzylamide sulfonates (CMDBS), have been immobilized on silica beads. By high-performance liquid affinity chromatography (HPLAC), they allow a good recovery of thrombin, with a yield of 80%. The affinity constant was estimated K lO M ) and was found superior to the value obtained between thrombin and heparin. On the contrary, the affinity of dextran derivatives for ATIII is estimated at a lower value than that of heparin [17],... 

Ohlson, S., Hansson, L., Larsson, P-O., and Mosbach, K., High performance liquid affinity chromatography (HPLAC) and its application to the separation of enzymes and antigens. FEBS Lett. 93, 5-9 (1978). 

High performance liquid affinity chromatography (HPLAC) is a recently introduced form of HPLC and few commercial stationary phases are currently available the Beckman Fast Affinity stationary phase, stationary phases from LKB and Biorad and the Pierce High Performance Affinity stationary phase. Only a few reports are available of their use and in the past most affinity stationary phases have been prepared by proprietary methods in individual laboratories. An... 

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