High-performance liquid affinity chromatography

Lipophilicity represents the affinity of a molecule or a moiety for a lipophilic environment. It is commonly measured by its distribution behavior in a biphasic system, either liquid-liquid (e.g. partition coefficient in 1-octanol-water) or solid-liquid (retention on reversed-phase high-performance liquid chromatography or thin-layer chromatography system). 

Baker et al. [137] reported that Rhesus monkeys were administered primaquine (6-10.5 mg as the phosphate/kg intravenously) and plasma samples were analyzed by high performance liquid chromatography for the presence of the unchanged drug and the major metabolite, 8-[3-carboxy-l-methylpropylamino)-6-methoxyquinoline. Primaquine had an unusually high affinity for tissue compartments, which produced a rapid initial drop in plasma concentration. Within 15 min, the plasma concentration of the metabolite far exceeded that of primaquine. Thirty-five to eighty-three percent of the primaquine dose was converted to the major metabolite. This metabolite possessed much lower affinity for the tissues compartments than the drug itself. 

A Ahmed, H Ibrahim, F Pastore, DK Lloyd. Relationship between retention and effective selector concentration in affinity capillary electrophoresis and high-performance liquid chromatography. Anal Chem 68 3270—3273, 1996. 

Such a hapten-immobilizing affinity column was also operated on-line as part of a high-performance liquid chromatography (HPLC) system (13). This is a... 

Khasawneh MA, Vallano PT, Remcho VT. Affinity screening by packed capillary high performance liquid chromatography using molecular imprinted sorhents. II. Covalent imprinted polymers. J Chromatogr A 2001 922 87-97. 

Kariluoto, M. S., Vahteristo, L. T, Piironen, V. 1. (2001). Applicability of microbiological assay and affinity chromatography purification followed by high-performance liquid chromatography (HPLC) in studying folate contents in rye. J. Sci. Food Agric., 81, 938-942. 

Schonmann and Kern [33] have used on-line enrichment for microgram per litre analysis of PAHs in water by high performance liquid chromatography. The trace enrichment method is based on the affinity of non polar pollutants for reversed phase chromatography supports. 

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