High-performance liquid affinity

J. B. Wheatley, J. A. Montali and D. E. Schmidt-Jr, Coupled affinity-reversed-phase high-performance liquid cliromatography systems for the measurement of glutathione 5-transferases in human tissues , 7. Chromatogr. A 676 65 - 79 (1994). 

B. Nilsson, Exti action and quantitation of cortisol by use of high-performance liquid affinity cliromatogr aphy , J. Chromatogr. 276 413-417 (1983). 

Lipophilicity represents the affinity of a molecule or a moiety for a lipophilic environment. It is commonly measured by its distribution behavior in a biphasic system, either liquid-liquid (e.g. partition coefficient in 1-octanol-water) or solid-liquid (retention on reversed-phase high-performance liquid chromatography or thin-layer chromatography system). 

Baker et al. [137] reported that Rhesus monkeys were administered primaquine (6-10.5 mg as the phosphate/kg intravenously) and plasma samples were analyzed by high performance liquid chromatography for the presence of the unchanged drug and the major metabolite, 8-[3-carboxy-l-methylpropylamino)-6-methoxyquinoline. Primaquine had an unusually high affinity for tissue compartments, which produced a rapid initial drop in plasma concentration. Within 15 min, the plasma concentration of the metabolite far exceeded that of primaquine. Thirty-five to eighty-three percent of the primaquine dose was converted to the major metabolite. This metabolite possessed much lower affinity for the tissues compartments than the drug itself. 

A third area of development in carbohydrate l.c. analyses is in the combined techniques (see Section IV,3) and other methods that provide qualitative, as well as quantitative, information about sample constituents, such as high-performance liquid affinity chromatography. The use of specific lectin- and monoclonal antibody-based, stationary phases for analytical and preparative applications is now being considered. The basic concepts of these techniques have been reviewed - and their applications to carbohydrates have been discussed. 

A Ahmed, H Ibrahim, F Pastore, DK Lloyd. Relationship between retention and effective selector concentration in affinity capillary electrophoresis and high-performance liquid chromatography. Anal Chem 68 3270—3273, 1996. 

Wikstrom, P. and Larsson, P.-O., Affinity fibre—A new support for rapid enzyme purification by high-performance liquid affinity chromatography, J. Chromatogr., 388, 123-134, 1987. 

Glad, M., Ohlson, S., Hansson, L., Mansson, M.-O., and Mosbach, K., High-performance liquid affinity chromatography of nucleosides, nucleotides and carbohydrates with boronic acid-substituted microparticulate silica, J. Chromatogr., 200, 254-260, 1980. 

Such a hapten-immobilizing affinity column was also operated on-line as part of a high-performance liquid chromatography (HPLC) system (13). This is a... 

Khasawneh MA, Vallano PT, Remcho VT. Affinity screening by packed capillary high performance liquid chromatography using molecular imprinted sorhents. II. Covalent imprinted polymers. J Chromatogr A 2001 922 87-97. 

Kariluoto, M. S., Vahteristo, L. T, Piironen, V. 1. (2001). Applicability of microbiological assay and affinity chromatography purification followed by high-performance liquid chromatography (HPLC) in studying folate contents in rye. J. Sci. Food Agric., 81, 938-942. 

G Stubbings, JA Tarbin, G Shearer. Online metal chelate affinity chromatography cleanup for the high-performance liquid chromatographic determination of tetracycline antibiotics in animal tissues. J Chromatogr B 679 137-145, 1996. 

Ohlson, S., Nilsson, R., Niss, U., Kjellberg, B. M., and Freiburghaus, C. (1988) A novel approach to monoclonal antibody separation using high performance liquid affinity chromatography (HPLAC) with SelectiSpher-10 protein G. J. Immunol. Methods 114,175-180... 

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