High performance affinity chromatography

The single most important advance in the purification of biomolecules in the last 20 years has been the introduction of affinity chromatography (Cuatrecasas et al., 1968). Affinity chromatography, as discussed 

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High performance adhesives, 1 545 High performance affinity chromatography support, 6 394-395... 

The ultimate combination of HPLC and AC is effectuated in High Performance Affinity Chromatography (HPAC).47 The development of this hybrid technique was highly assisted by the use of modified silica. Traditional polysaccharide supports may not be used for HPAC, because they lack mechanical stability to withstand the high pressure drops, inherent to this method. Modified silica beads are well suited. These may be coated with active groups as in normal AC applications. Additionally, if the separation requires the use of an organic stationary phase, the silica beads are modified with a silane or polymer with subsequent deposition of polysaccharides such as dextrans, agarose or cellulose.50... 

A. S. Clairbois, D. Letourneur, D. Muller, and J. Joze-fonvicz. High-performance affinity chromatography for the purification of heparin-binding proteins from detergent-solubilized smooth muscle cell membranes, J. Chromatogr. B 706 55 (1998). 

High-performance affinity chromatography employs bonded-phase silica with particle sizes of 10 pm, having pore sizes of 300 A, as well as nonporous particles of 1.5-pm diameter or less. With these support media, pressures in excess of 2500 psi may be used, and these are the pressures commonly encountered in HPLC systems. 

Anderson DJ, Branum EL, O Brien JF. Liver- and bone-derived isoenzymes of alkaline phosphatase in serum as determined by high-performance affinity chromatography. Clin Chem 1990 36 240-6. 

Hage, D. S., NoctopT. A. G.,Wainer, I. W. Characterization of the protein binding of chiral drugs by high-performance affinity chromatography. Interactions of... 

Hage, D. S. High-performance affinity chromatography a powerful tool for studying serum protein binding,... 

Hage, D.S. and Austin, J., High-performance affinity chromatography and immobilized serum albumin as probes for drug- and hormone-protein binding, J. Chromatogr. B, 739, 39, 2000. 

Interaction Mechanism of Thrombin with Functional Polystyrene Surfaces A Study Using High-Performance Affinity Chromatography... 

In the present paper, we report high-performance affinity chromatography of thrombin in presence of AT III and Hep, using two types of resins as stationary phases either heparin-like PSSO or AT Ill-like PAOM. In order to differentiate their mechanisms of interaction with thrombin, we examined the chromatographic behavior of thrombin in the presence, or in the absence of AT III and/or heparin. Finally, thrombin was injected on the columns at low ionic strength. The desorption of bound thrombin from the two solid surfaces was then carried out using AT III, heparin and the AT Ill-Hep complex, to elucidate the specificity of the interactions involved. 

Walters, R.R. (1982) High-performance affinity chromatography. Pore size effects./. Chromatogr., 249, 19-28. 

J. Chen, C. Phnmacht and D. S. Hage, Studies of phenytoin binding to human serum albumin by high-performance affinity chromatography, /. Chromatogr., A, 2004, 809,137-145. 

K. Yasukawa, F. Abe, N. Shida, Y. Koizumi, T. Uchida, K. Noguchi and K. Shima, High-performance affinity chromatography system for the... 

High-performance affinity chromatography HPAC) is characterized by a support that consists of small, rigid... 

Source From Studies of phenytoin binding to human serum albumin by high-performance affinity chromatography, in J. Chromatogr. 

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