Vector construction

Wall ID, Rapp-Giles BJ, Ronsset M. 1993. Characterization of a small plasmid from Desulfovibrio desulfuricans and its nse for shnttle vector construction. J Bacteriol 175 4121-8. 

J. Hodgson, Expression systems a user s guide. Emphasis has shifted from the vector construct to the host organism, Bio/Technology 1993, 11, 887-893. 

Production of vector virions requires transfection of the vector construct in plasmid form together with the introduction on separate plasmids and/or helper viruses of both the AAV genes and the helper virus genes required for a productive AAV infection (Xiao et al., 1998) (Fig. 1.1). Variations have included producing vectors... 

Vectors constructed with such proteolytic linkers can thus be released from their target during panning with a mild buffer containing the appropriate protease, without having to guess at optimal elution conditions (see Ref. 85 thrombin cleavage for elution). 

Kim, S. C., Posfai, G., and Szybalski, W. (1991). A novel gene-fusing vector construction of a 5 -GGmCGspecific chimeric methyltransferase, M-BspRl/M-IlsuRl. Gene, 100, 45-50. 

Great efforts have been made to develop the preparation of antibiotics by novel biotechnological methods. In order to increase productivity of the wide spectrum of Streptomyces strains, experts developed a novel vector construction in the Cephalosporium fungus. Streptomyces vectors were also applied for the transformation of Micromonospora strains. By optimization of the essential parameters of fermentation, 15-20% higher Cephalosporin C production has been attained, compared to the results of the original technology. 

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