Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Acids identification

Gas Chromatography (gc). A principal advantage of gas chromatography has been the faciUty with which it can be combined with mass spectrometry for amino acid identification and confirmation of purity. The gc-mass spectrometry combination offers the advantage of obtaining stmctural information rather than the identification by retention time in hplc. [Pg.284]

Sanger s reagent (Section 27.11) Thecompound l-fluoro-2,4-dinitrobenzene, used in N-terminal amino acid identification. [Pg.1293]

Humans can synthesize 12 of the 20 common amino acids from the amphiboHc intermediates of glycolysis and of the citric acid cycle (Table 28-1). While nutritionally nonessenrial, these 12 amino acids are not nonessential. AH 20 amino acids are biologically essential. Of the 12 nutritionally nonessential amino acids, nine are formed from amphibolic intermediates and three (cysteine, tyrosine and hydroxylysine) from nutritionally essential amino acids. Identification of the twelve amino acids that humans can synthesize rested primarily on data derived from feeding diets in which purified amino acids replaced protein. This chapter considers only the biosynthesis of the twelve amino acids that are synthesized in human tissues, not the other eight that are synthesized by plants. [Pg.237]

Dansyl chloride (dimethylaminonaphthalene-5-sulphonyl chloride) will react with free amino groups in alkaline solution (pH 9.5-10.5) to form strongly fluorescent derivatives (Figure 10.14). This method can also be used in combination with chromatographic procedures for amino acid identification in a similar manner to the FDNB reagent but shows an approximately 100-fold increase in sensitivity. This makes it applicable to less than 1 nmol of material and more amenable for use with very small amounts of amino acids liberated after hydrolysis of peptides. The dansyl amino acids are also very resistant to hydrolysis and they can be located easily after chromatographic separation by viewing under an ultraviolet lamp see Procedure 10.1. [Pg.359]

Bhown, A.S., J.E. Mole, and J.C. Bennett, improved procedure for high-sensitivity microsequencing use of aminoethyl amino-propyl glass beads in the Beckman sequencer and the ultrasphere ODS column for PTH amino acid identification. Anal Biochem, 1981.110(2) 355-9. [Pg.60]

Ha, Y.L., Grimm, N.K.. and Pariza, M.W. (1989) Newly recognized anticarcinogenic fatty acids. Identification and quantification in natural and processed cheeses. J. Agric. Food Chem., 37, 75-81. [Pg.297]

The dansyl analysis was thereafter directed toward obtaining an NH2-terminal amino acid identification for each of these five major peaks (in Fig. 4.6). In each case, the NH2-terminus was identified by two separate thin-layer chromatographic methods... [Pg.73]

NH2-terminal amino acid identification of the molecular subclasses Composing (dansylated) microbubble glycopeptide surfactant. (Taken from ref. 322.)... [Pg.90]

Sanches-Silva, A., Rodriquez-Bernaldo de Quiros, A., Lopez-Hemandez, J., Paseiro-Losada, P. 2004. Comparison between high-performance liquid chromatography and gas chromatography methods for fatty acid identification and quantification in potato crisps. J. Chromatogr. A. 1032, 7—15. [Pg.695]

Labeling Indicate the concentration of Lactic Acid. Identification A sample gives positive tests for Lactate, Appendix IIIA. [Pg.240]

Conrad, F., Hanne, A., Gaur, R.K. and Krupp, G. (1995). Enzymatic synthesis of 2 -modified nucleic acids identification of important phosphate and ribose moieties in RNase P substrates. Nucleic Acids Res. 23, 1845-1853. [Pg.54]

K Kassahun, P Hu, MP Grillo, MR Davis, L Jin, TA Baillie. Metabolic activation of unsaturated derivatives of valproic acid. Identification of novel glutathione adducts formed through coenzyme A-dependent and -independent processes. Chemico-Biol Int 90 253, 1994. [Pg.192]

The major problem in food analysis is the complexity of the matrices. For example, coffee has a complex matrix. Acids from coffee are important for the sensory quality of the coffee beverage. For every acid identification, it is necessary to perform an electrophoretic cleanup of all organic acids followed by the use of the GC-MS technique.34... [Pg.21]

There are numerous references in the literature to the preparation of quinoxalinones as characterizing derivatives for a-keto acids. Paper chromatographic, " gas-liquid chromatographic, and fluorimetric methods have been used for the microestimation of quinoxalinones. Quinoxalinone formation is also the basis of a method for amino acid identification, as shown in Scheme 18. It has found application in... [Pg.93]

Peak identification and potential coelution of organic acids are not always given due consideration, although AEC has much inferior separation efficiencies compared to GC. Ideally, organic acid identification, which is typically done by retention time comparison, should be confirmed with another independent method. Several publications investigating rain and atmospheric precipitation validated their AEC results with either lEC or Other... [Pg.487]


See other pages where Acids identification is mentioned: [Pg.638]    [Pg.403]    [Pg.182]    [Pg.72]    [Pg.74]    [Pg.232]    [Pg.81]    [Pg.106]    [Pg.175]    [Pg.114]    [Pg.2017]    [Pg.52]    [Pg.103]    [Pg.104]    [Pg.589]    [Pg.708]    [Pg.356]    [Pg.403]   
See also in sourсe #XX -- [ Pg.1274 , Pg.1275 ]




SEARCH



Acetic acid bacteria identification

Acid anhydrides, detection identification

Acid chlorides, detection identification

Acids progressive identification

Acids, aromatic identification

Amino acid identification

Amino acid residue identification/sequence

Amino acid residues identification

Amino acid sequences analysis/identification

Amino acid sequences identification

Carboxylic acid derivatives identification

Carboxylic acids, addition compounds with identification

Cyanuric acid, identification

Deoxyribonucleic acid identification

Esters, simple, identification of acidic

Esters, simple, identification of acidic components

Experiment 61 Identification of Amino Acids in Food by Paper Chromatography

Fatty acids, identification

Functional groups: chemical identification acidic compounds

Histochemical Identification of Sialic Acids

Hydroxy acids, detection identification

Identification of Amino Acid Residues Relevant to Substrate Specificity and Enantioselectivity

Identification of Phosphoamino Acids (Paper Electrophoresis)

Identification of acids

Identification of sulfonic acid

Keto acids, detection identification

LABORATORY PROCEDURES FOR IDENTIFICATION OF ACETIC ACID BACTERIA

Lactic acid bacteria identification

Lactic acid bacteria strain identification

Modified amino acids, identification

Quantitation and Identification of Proteins by Amino Acid Analysis ABRF-96AAA Collaborative Trial

Sulfonic acid chlorides, identification

Sulfonic acids identification

Sulfonic acids, detection identification

Sulphonic acids identification

Uronic acids identification

© 2024 chempedia.info