Amino acid residues identification

An alternative approach to peptide sequencing uses a dry method in which the whole sequence is obtained from a mass spectrum, thereby obviating the need for multiple reactions. Mass spec-trometrically, a chain of amino acids breaks down predominantly through cleavage of the amide bonds, similar to the result of chemical hydrolysis. From the mass spectrum, identification of the molecular ion, which gives the total molecular mass, followed by examination of the spectrum for characteristic fragment ions representing successive amino acid residues allows the sequence to be read off in the most favorable cases. 

Hemanz-Falcon P, Rodriguez-Frade JM, Serrano A, et al. Identification of amino acid residues crucial for chemokine receptor dimerization. Nat Immunol 2004 5(2) 216-223. 

V. Monchois, M. Remaud-Simeon, R. R. Russell, P. Monsan, and R. M. Willemot, Characterization of Leuconostoc mesenteroides NRRL B-512F dextransucrase (DSRS) and identification of amino-acid residues playing a key role in enzyme activity, Appl. Microbiol. Biotechnol., 48 (1997) 465 -72. 

SUH, D.-Y., FUKUMA, K., KAGAMI, J., YAMAZAKI, Y., SHIBUYA, M., EBIZUKA, Y., SANKAWA, U., Identification of amino acid residues important in the cyclization reactions of chalcone and stilbene synthases, Biochem. J., 2000,350, 229-235. 

Tan J, Soderlund DM (2005) Identification of amino acids residues in the insect sodium channel critical for pyrethroids binding. Mol Pharmacol 67 513-522... 

Grutter T, Ehret-Sabatier L, Kotzyba-Hibert E, Goeldner M. 2000. Photoaffinity labelling of Torpedo nicotinic receptor with the agonist [ H] DCTA identification of amino acid residues which contribute to the binding of the ester moiety of acetylcholine. Biochemistry 39 3034-3043. 

Somatropin is a protein having the structure (191 amino acid residues) of the major component of growth hormone produced by the human pituitary. Somatropin is produced by a method based on recombinant DNA technology. Recently the results of a collaborative study between 14 laboratories have been reported, in which a new CE method was compared with the existing test for isoform distribution by isoelectric focusing. The CE method was found to be superior to the method of isoelectric focusing. As a result CE is applied for identification... 

Stuible, H.P. and Kombrink, E., Identification of the substrate specificity-conferring amino acid residues of 4-coumarate coenzyme A ligase allows the rational design of mutant enz5mes with new catalytic properties. J. Biol Chem., 276, 26893, 2001. 

Marsolais, E. and Varin, L., Identification of amino acid residues critical for catalysis and cosubstrate binding in the fiavonol 3-sulfotransferase. J. Biol Chem., 270, 30458, 1995. 

Identification of Amino Acid Residues Relevant to Substrate Specificity and Enantioselectivity... 

---