Acetone elute

Addition of acetone/ Elution with 3 ml Addition of EtOH/ Elution with 3 ml EtOH Elution with 4 ml... 

Alternatively, a measured volume of air drawn through a prepacked cartridge coated with acidified DNPH the DNPH derivative of acetone eluted with acetonitrile and measured by HPLC-UV as above (U.S. EPA Method TO-11, 1988). 

Extract sample with acetone elute from HPLC column with methanol/potassiurn phosphate... 

For solvent desorption, adsorbents such as modified silica containing cyanopropyl groups with acetone elution and GC-FID/GC-MS detection (Woitowitz and Knecht,... 

Atmosphere adsorption on polyurethane foam (desorption by Soxhlet extraction with petroleum ether) alumina fractionation (elution with 2 1 MeOH/ CH2CI2) water. SPE on XAD-2 resin Matrix solid phase dispersion extraction with Cijj (MeOH elution) AI2O3 cleanup of MeOH eluate SPE on poly(styrene/divinylbenzene) disk (elution with acetone, MeCl2, hexane) NP-HPLC fractionation Water. SPE on Cig (acetone elution) sediment and algae Soxhlet extraction with hexane cleanup by SPE NH2 media (elution with 75 25 hexane/acetone)... 

Acrolein is produced according to the specifications in Table 3. Acetaldehyde and acetone are the principal carbonyl impurities in freshly distilled acrolein. Acrolein dimer accumulates at 0.50% in 30 days at 25°C. Analysis by two gas chromatographic methods with thermal conductivity detectors can determine all significant impurities in acrolein. The analysis with Porapak Q, 175—300 p.m (50—80 mesh), programmed from 60 to 250°C at 10°C/min, does not separate acetone, propionaldehyde, and propylene oxide from acrolein. These separations are made with 20% Tergitol E-35 on 250—350 p.m (45—60 mesh) Chromosorb W, kept at 40°C until acrolein elutes and then programmed rapidly to 190°C to elute the remaining components. 

Adsorption of lA of POMs with CV and Malachite Green (MG) on the polyurethane foams (PF) and some other adsorbents is investigated. While lA is fully adsorbed on the PF in wide pH range (0,4 M H SO - pH 4) extent of dye adsoi ption does not exceed 0,4%. lA are adsorbed faster then POMs. Extent of sorption of lA is 60-70% at 5 minutes and is complete after 15 minutes. lA can be eluted from PF most effectively by methylbutylketone, acetone or alcohols can be used too. 

This material Is purified by recrystallization from ethyl acetate acetone 2 1 (v v) to give a first crop (6.8 g), and by flash chromatography of the residue from the mother liquor, using 150 g of 230-400 mesh silica gel (Merck), a 40-mm diameter column, and elution with 10 1 (v v) ethyl acetate methanol. A fast moving orange band and a slower moving lemon-yellow band can be clearly seen on the column. The lemon-yellow hand is collected from the column and evaporation gives a second crop (1.4 g) of comparably pure material. The total yield of the pale yellow isoquinoline is 8.2 g (86t), mp 135-137°C (Note 10). 

Carbon Black Leached for 24h with 1 1 HCl to remove oil contamination, then washed repeatedly with distd water. Dried in air, and eluted for one day each with benzene and acetone. Again dried in air at room temp, then heated in a vacuum for 24h at 600 to remove adsorbed gases. [Tamamushi and Tamaki Trans Faraday Soc 55 1007 7959.]... 

Dioctadecyldimethylammonium bromide [3700-67-2] M 630.9, m 161-163 . Crystd from acetone then MeOH [Lukac J Am Chem Soc 106 4387 1984]. Also purified by chromatography on alumina by washing with CgHg and eluting with Me2CO, evap and cryst from MeCN [Swain and Kreevoy J Am Chem Soc 77 1126 1955]. 

Trisodium 8-hydroxy-l,3,6-pyrenetrisulfonate [6358-69-6J M 488.8, m >300(dec). Purified by chromatography with an alumina column, and eluted with -propanol-water (3 1, v/v). Recrystd from aqueous acetone (5 95, v/v) using decolorising charcoal. 

It has been recrystd from H2O (fine needles) and is freely soluble in boiling H2O. Crysts also from H2O by addition of acetone. Purified by chromatography on Dowex 1 (in formate form), eluting with 0.25M formic acid. It was then adsorbed onto charcoal (which had been boiled for 15min with M HCI, washed free of chloride and dried at 100°), and recovered by stirring three times with isoamyl alcohol/H20 (1 9 v/v). The aqueous layer from the combined extracts was evaporated to dryness under reduced pressure, and the product was crystallised twice from hot H2O. [Morrison and Doherty Biochem J19 433 7967]. It has A-max 259nm (e 15,400) in H2O at pH 7.0. [Alberty et al. J Biol Chem 193 425 7957 Martell and Schwarzenbach Heh Chim Acta 39 653 7956]. The acridinium salt has m 208° [Baddiley and Todd J Chem Soc 648 1947 Pettit Synthetic Nucleotides, van Nostrand-Reinhold, NY, Vol 1 252 1972 NMR Sarma et al. J Am Chem Soc 96 7337 1974 Norton et al. J Am Chem Soc 98 1007 1976 IR of diNa salt Miles Biochem Biophys Acta 27 324 1958],... 

Commercial material contains up to 4% desmethylcolchicine. Purified by chromatography on alumina, eluting with CHCI3 [Ashley and Harris J Chem Soc 677 1944]. Alternatively, an acetone solution on alkali-free alumina has been used, and eluting with acetone [Nicholls and Tarbell J Am Chem Soc 75 1104 1953]. 

A solution of 1 g of the dione in 200 ml of methanol at 0° is treated with 75 mg of sodium borohydride and the mixture is kept for 2 hr. After addition of 0.1 ml of acetic acid the mixture is concentrated to ca. 20 ml. Dilution with water gives 0.9 g of crystals which are chromatographed on 20 g of unwashed alumina. Elution with benzene-ether (40 60) yields 0.73 g of the methyl-hydroxytestosterone, mp 245-249°, which after crystallization from acetone has mp 255-256° [a] 111° (CHCI3). 

The mixture is cooled to room temperature, then filtered. The solvent is removed under reduced pressure, leaving the tribromide (47) as a foam. The foam is mixed with sodium iodide (9.55 g, 0.064 mole) and acetone (74 ml) and heated under reflux in a nitrogen atmosphere for 3.5 hr. The acetone is removed under reduced pressure and the residue is treated with chloroform and aqueous sodium thiosulfate solution. The chloroform layer is separated and washed with sodium thiosulfate solution until it is free from iodine, then dried over magnesium sulfate, filtered and evaporated to dryness under reduced pressure. The crude product (48) is obtained as a brown sohd (4.85 g) which is chromatographed over alumina (122 g, Merck acid-washed). The column is developed with hexane, benzene and ethyl acetate mixtures. The product (3.43 g) is eluted by benzene and benzene-ethyl acetate (10 1). Recrystallization from acetone yields purified 3jS-acetoxy-pregna-5,14,16-trien-20-one (48), 3.25 g, mp 158-159° 309 m/ (e 10,700). 

A solution of cholest-4-en-3-one (139), 1 g, in diethylene glycol dimethyl ether (20 ml) is treated for 1 hr with a large excess of diborane at room temperature under nitrogen and then left for a further 40 min. Acetic anhydride (10 ml) is added and the solution refluxed for 1 hr. The mixture is concentrated to a small volume, diluted with water and extracted with ether. The extracts are washed with 10% sodium hydroxide solution, then with water and dried over sodium sulfate. Removal of the solvent leaves a brown oil (1.06 g) which is purified by chromatography on alumina (activity I). Hexane elutes the title compound (141), 0.68 g mp 76-77°. Successive crystallization from acetone-methanol yields material mp 78-79°, [a]p 66°. 

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