3T3 fibroblast cells

In the EST, two murine cell lines are used to assess teratogenic potential the embryonic D3 stem cell (ES) which represents the embryonic tissue and the 3T3 fibroblast cell which represents the adult tissue. The D3 cells are maintained in an undifferentiated stage in the presence of leukemia inhibiting factor (LIE), then released from LIE and allowed to form embryo bodies that differentiate into cardiomyocytes. The D3 and 3T3 cells are exposed to a range of concentrations of the test ingredient. After a 10-day... 

Schatz, D.G. Baltimore, D. (1988). Stable expression of immunoglobulin gene V(D)J recombinase activity by gene transfer into 3T3 fibroblasts. Cell 53,107-115. 

Like the other 5-Hrl receptors, the major signaling pathway of the 5-HT1D receptor is to the inhibition of AC through pertussis toxin-sensitive G proteins in the substantia nigra (153), in MDCK cells (154), and in transfected C6 glioma and NIH-3T3 fibroblast cells (116,134,155,156). 

Fig. 3.11 (a) Averaged Raman spectrum of 50 different NIH/3T3 fibroblast cells measured at a 785 nm excitation wavelength, 50 mW laser power at the sample for 180 s. (b-e) SER spectra taken by excitation of NS in different cells at the same wavelength with 5 s acquisition time and 3 mW laser power at the sample [56]... 

Fig. 3.12 (a) TEM image taken of a single NS in NIH/3T3 fibroblast cell located near the nuclear membrane, (b) SERS spectra of 4 mercaptobenzoic-acid functionalized NS in NIH/3T3 fibroblast. Overlying spectra depict NS-4MBA in solution black) and inside NIH/3T3 cells red) determining the pH to be 7.4 and 6.5, respectively [56]... 

Lithium uptake experiments in erythrocytes may have value in the prediction of those patients who are most likely to respond to treatment (114-117). Other studies have used squid axon, hepatocytes, 3T3 fibroblast cell cultures, and liposomes to investigate lithium transport across the plasma membrane (77, 118). 

HT29-5M21 cells, seeding ratios influenced the paracellular permeability of the cell monolayer [56], Adult rat hepatocytes maintained on top of a mesenchymal progenitor cell line C3H/10T1/2 cells [57] or rat 3T3 fibroblast cells [58] maintained better viability and higher ethoxyresorufin O-dealkylase (EROD) activity and albumin secretion functions [58],... 

Conrad, P.A., Nederlof, M.A., Herman, l.M. et al. (1989). Correlated distribution of actin, myosin, and microtubules at the leading edge of migrating Swiss 3T3 fibroblasts. Cell Motil. Cytosheleton 14, 52T--543. 

Cell culture studies have shown that HEMA is unquestionably cytotoxic towards cells [75,76]. In one study using the resin-modified glass-ionomer Vitremer, severe cytotoxic effects were shown to occur on exposure to mouse 3T3 fibroblast cells [77]. Surprisingly, the authors attributed this finding to release of the monomer TEGDMA, but in view of the relative amounts of monomers released, and their generally known biological effects, it seems more likely that HEMA was responsible. 

T3 Fibroblast cells (American Type Culture Collection, Accession Number CCL 92). 

Levi-Schaffer, F. and Riesel, N. (1989) In vitro regeneration of activated rat peritoneal mast cells cocultured with 3T3 fibroblasts. Cell Immunol. 119, 30-40. 

Kg. 14 Optical micrograph showing the adhesion of 3T3 fibroblast cells on a MUA-MUD-derived FN-BSA gradient prepared by electrochemical desorption. The top and bottom x-axes show the spatial/potential... 

POMC films promoted the adhesion and proliferation of human aortic smooth muscle cells and NIH-3T3 fibroblast cell lines and demonstrated minimal inflammatory response when subcutaneously implanted in Sprague-Dawley rats. The success in... 

The NIH 3T3 fibroblast cell line was chosen for this study because it provides a robust and durable platform for investigating common cellular functions attachment, viability, proliferation, cellular properties, membrane states, etc.( 14). Figure 5 illustrates the morphology of mouse fibroblast 3T3 cells cultured on a PCL film and PAOS films. To a first, visual approximation cells grown on the sorbitol-containing polyester films and the PCL control exhibit no morphological differences. This indicates qualitatively comparable biocompatibility between PAOS and PCL. 

Okai, Y, Higashi-Okai, K., Yano, Y, Otani, S., 1996. Suppressive effects of chlorophyllin on mutagen-induced umu C gene expression in Salmonella typhimurium (TA 1535/pSK 1002) and tumor promoter-dependent ornithine decarboxylase induction in BALB/c 3T3 fibroblast cells. Mutation Research/Genetic Toxicology 370 (1), 11-17. 

Abstract— In the present study we show that the improved proliferation of the 3T3 fihrohlast cell line and the cells attachment could be obtained when applied a suitable chitosan solution as additive and supplementing ingredient in the cell culture medium of DMEM with 10% FBS, SO U/ml Penicillin, SO pg/ml Streptomycin, SO pg/ml Neomycin. In addition, the 3T3 Fibroblast cells formed clear cell colonies in the cultured medium. By applying a concentration of chitosan solution of 3Sppm with a volume ratio of 1 3 to the standard cell culture medium, the cell growth of the 3T3 cell line was increased up to about 33.33% comparing to medium without chitosan solution. 

Keywords— cell line, chitin, chitosan, DMEM, shrimp shells, 3T3 fibroblast cells. 

Efforts has been made to look for applicability of CTS solution by using it in cell culture as an additive and a supplementing ingredient in culturing medium of the 3T3 fibroblast cell line. Scientifically, it is necessary to investigate the effect of chitosan solution in cell culture and applying to 3T3 fibroblast cell line is as a good example. In this paper successful application of chitosan solution is reported. 

T3 fibroblast cell lines (provided by the Laboratory of Research and Applied Stem Cells, University of Natural Sciences, Ho Chi Minh City). 

The 3T3 fibroblast cell line was provided by the Lab of Stem Cell at the University of Natural Sciences which was originated from the Sigma snpplier (Sigma 93061524). Cell at passage of 4-6 was nsed for the experiments. The cells were already sub-cnltnred in flask containing of DMEM (plus 10% raS), 50U/ml Penicilhn, 50pg/ml Streptomycin and SOpg/ml Neomycin. 

Application of Shrimp Chitosan Solution as Additive and Supplementing Ingredient in Culturing 3T3 Fibroblast Cells... 

The study demorrstrated that the improved proliferation of the 3T3 fibroblast cell lirre arrd the cells attachment could be obtained when applied a suitable chitosan solution as... 

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