Zeiss microscope

Figure 1. Schematic diagram of the Zeiss microscope-computer instrumentation.

The optical setup for time-resolved micro-luminescence measurements is based around an Axiotech 100 HD Zeiss microscope, modified to allow laser injection and fluorescence collection. The sample is observed either under transmission or reflection of polarized white light, or under UV illumination (HBO lamp). A set-up consisting of a dichroic mirror, for the selection of the excitation wavelength, and an objective (Epiplan Neofiuar obj. > 350 nm Ealing/Coherent reflection obj. < 350 nm) is used to focus the laser beam on the sample (spatial resolution over 5 pm with a x50 objective). The lim-... 

Recently, an automatic color video image analysis system was developed to quantify antigen expression (androgen receptor) (Kim et al.,T999a). This system provides a linear relationship between the antigen content and mean optical density of the immunoperoxidase-substrate reaction product. Titration of antibody, concentration, and reaction duration of the substrate can be optimized with this system. The imaging hardware consists of a Zeiss microscope, a three-chip charge-coupled-device camera, a camera control board, and a Pentium-based personal computer. 

The procedure was as described (5/11/12). In short/ the absorption spectrum of a bleb suspension was measured/ and the overall extinction coefficients were calculated by dividing the absorbances by the total chlorophyll concentration. The absorption spectrum of single blebs was recorded in a Zeiss microscope spectrophotometer. The chlorophyll content of a bleb was estimated by fitting its spectrum to the overall extinction coefficients. Its membrane surface was calculated from its diameter/ and the specific surface was obtained as ratio of surface to chlorophyll content. 

UV-vis absorption spectra were recorded on a Perkin-Elmer UV-Vis-NIR spectrometer Lambda 950, in a 1 cm optical path cuvette, with a data interval equal to 1 nm and a Scan Speed of 20 nm/mia Samples for SEM examinations were prepared by filtering a small amount of the colloidal solution on Anodisk membranes with a pore size of 20 nm. SEM micrographies of membranes loaded with AgNP were taken with an EVO ZEISS microscope, with an EHT of 25 kV. In order to limit filtering-related aggre tion phenomena, after the preparation of samples a and b, we reduced the quantity filtered thus obtaining a better dispersion. 

Somatostatin. Figure 1 Somatostatin-like im mu noreactivity in neurons of the periventricular hypothalamic nucleus of the rat. Coronal brain cryostat sections have been processed for im mu nohistochemistry and sequentially incubated with a primary monoclonal mouse anti human somatostatin antibody and secondary antimouse antibody conjugated with the fluorescence-dye Cy-3. Images have been taken with a Zeiss Axioplan fluorescence microscope. Scale bar, 100 pM. 

Fluorescence microscopy measurements were performed with a Zeiss Axioplan microscope (Zeiss Co. Germany) equipped with a mercury lamp and a 40X objective. Images were acquired by CCD camera CH250 (Photometrix Co., Germany) cooled at -40°C by a liquid cooling unit CH260 (Photometrix Co., Germany). 

Another important lesson from Table 8.1 is that all three of the methods tested yielded virtually the same FRET efficiencies for the same samples. The sRET method as implemented used two-photon excitation on a Zeiss 510 META/NLO microscope, as did the FLIM-FRET method, but FLIM-FRET used auxiliary time... 

To implement TIRF, we needed two modihcations hrstly we incorporated a Zeiss a-Plan Fluar 100 x NA 1.45 oil objective, and secondly, we modihed the conhguration of the output of the multi-mode hber to incorporate off axis coupling into the microscope. By using a micromanipulator moving the output of the hber off axis... 

Analyze the protoplasts under a fluorescence microscope suitable for FRET measurements (e.g., Leica SP2/SP5 or Zeiss LSM 510 Meta CLSM). 

III. Transmission electron microscopy of radish seeds Transmission electron microscopy (TEM) of radish seeds was done as listed below For TEM preparations, the specimens after fixation and dehydration, were embedded in Epon 812 resin (Luft, 1961). Thick sections (ca. 1mm each) were stained with 0.1% toluidine blue and observed with a Zeiss light photomicroscope. Thin sections, obtained with a diamond knife on a Supernova microtome, were sequentially stained at room temperature with 2% uranyle acetate (aqueous) for 5 min and by lead citrate for 10 min (Reynolds, 1963). Ultrastructural studies were made using a Philips CM12 transmission electrone microscope (TEM) operated at 80 KV. 

Materials required Carl Zeiss Laser Scanning Confocal Microscope LSM 510 NLO Carl Zeiss, object glasses, cover glasses, plant allelochemicals and pigments, azulene, quercetin and rutin, rutacridone, chlorophyll, sum of carotenoids... 

BT 20 cells incubated in serum free medium for 10 hours with the vector/ DNA complexes (DQAplexes, C-DQAplexes). For control, cells were exposed to naked DNA and empty vesicles. The cells were then stained with Mitotracker Red CMXRos (Molecular Probes) for five minutes to enable the visualization of mitochondria followed by confocal fluorescence microscopic analysis on a Zeiss Meta 510 Laser Scanning Microscope. 

Epifluorescence microscope equipped with appropriate filters (e.g., Zeiss Axioplan epifluorescence microscope with rhodamine filters). 

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