Mirrors, dichroic

M, dichroic mirror P, dispersive prism I, iris L, lens T, 5-axis fiber positioner PUT 1, detection photomultiplier tube ... 

Figure 4.6 Block diagram of the apparatus for the fluorescence depolarization measurement. The dashed and solid arrows indicate the light paths ofthe excitation pulse and the fluorescence from the sample. OBJ microscope objective, M mirror, L lens, DM dichroic mirror, LP long-pass filter, PH pin-hole, PBS polarizing beam splitter, P polarizer, PMT photomultiplier.

In this case, the excitation and emission filters and dichroic mirror used with the sample are removed and replaced with a beam splitter [3, 36], A scattering solution is placed on the microscope and a... 

Fig. 10.2. FSPIM analysis of the interaction between maize transcriptional coactivators—GCN5 and ADA2—fused to CFP and YFP. GCN5 is a histone acetyltransferase that, in conjunction with adaptor protein ADA2, modulates transcription in diverse eukaryotes by affecting the acetylation status of the core histones in nucleosomes [63]. CFP- and YFP-tagged proteins, expressed in protoplasts, were excited by the 458 nm and the 514 nm laser lines sequentially. CFP fluorescence was selectively detected by an FIFT 458 dichroic mirror and BP 470-500 band pass emission filter while YFP fluorescence was selectively detected by using an HFT 514 dichroic mirror and...

Fluorophores can be visualized in fluorescence microscopy using special filter blocks that are composed of the excitation filter, dichroic mirror and emission filter. The excitation filter must select wavelengths of light from a light source that fall in the maximum absorption region of the fluorophore. The emission filter must pass the fluorescent wavelengths but not the excitation wavelengths. The dichroic mirror... 

Dichroic mirror (or so called chromatic beam-splitter) reflects wavelengths of light below the transition wavelength value and transmits wavelengths above this value. 

Fig. n.1. Principles of fluorescence up-conversion. NLC nonlinear crystal DM dichroic mirror HW half-wave plate PM photomultiplier. 

Fig. 11.2. Fluorescence up-conversion instrument. DM dichroic mirror HW halfwave plate GG420 Schott filter CCD video camera for the visual superposition of the...

In a confocal microscope, invented in the mid-1950s, a focused spot of light scans the specimen. The fluorescence emitted by the specimen is separated from the incident beam by a dichroic mirror and is focused by the objective lens through a pinhole aperture to a photomultiplier. Fluorescence from out-of-focus planes above and below the specimen strikes the wall of the aperture and cannot pass through the pinhole (Figure 11.3). 

Figure 12. Laser configuration for SHG experiments, incorporating four single-narrow-stripe (SNS) red laser diodes and a prism (P) for wavelength tuning (DM dichroic mirror HWP half-wave plate PC polarization cube HR high reflector 1.5% output coupler).

Fluorescence microscope equipped with appropriate excitation filter, dichroic mirror, and barrier filter. For this example, an Olympus BHT compound microscope equipped with a BP490 excitation filter, BH-2DM500 dichroic mirror, and a LP515 barrier filter is used. 

FIGURE 2.1 A diagram of a multi-photon microscope. For AF, SFIG and exogenous probe fluorescence only one laser is used. The fluorescence or SHG signal passes through a dichroic mirror to the detector(s). One or two detectors and the appropriate filters can be used to collect multiple emission signals simultaneously. A spectrometer can be placed in the detector path to collect spectra, or a polarizer can be used to collect polarization data. 

The laser light travels through the epifluorescence or side port of the microscope. A dichroic mirror reflects the laser light and passes the green fluorescence to either of the detectors. Detectors are positioned on the bottom port of the inverted microscope or the top port of the upright microscope. The choice of detector is discussed in more detail below. Broadband and band-pass filters placed in the detection path prevent residual IR from reaching either of the detectors. 

CO Collection objective D Detector Daq Data aquisition Dm Dichroic mirror Eo Excitation objective E Eilter L Lens M Mirror Ph Pinhole... 

FIGURE 5.8 (a) CARS energy diagram, (b) Experimental setup BS, 15% beam splitter VA, variable attenuator A/2, half-waveplate Dl, 950 nm longpass dichroic mirror D2, 750 nm longpass dichroic mirror F, three 670 nm bandpass filters LI, aspheric lens L2, 10 cm concave lens. 

FIGURE 9.7 Schematic of an in-line interferometer. The anti-Stokes local oscillator field is collinearly overlapped with the pnmp and Stokes beams on a dichroic mirror (DM). All fields are focused by a microscope objective (MO) into the sample (S), and the total signal at the anti-Stokes frequency is detected throngh a spectral bandpass filter (F) at the photodetector. 

---