Yeast gene expression

Figure 6.27. Monitoring Changes in Yeast Gene Expression. This microarray analysis shows levels of gene expression for yeast genes under different conditions. [Adapted from Iyer et al., Nature 409(2000) 533.]...

Rossignol, T., Dulau, L., Julien, A., and Blondin, B. 2003. Genome-wide monitoring of wine yeast gene expression during alcoholic fermentation. Yeast 20,1369-1385. 

Varela, C., Cardenas, J., Melo, F., and Agosin, E. 2005. Quantitative analysis of wine yeast gene expression profiles under winemaking conditions. Yeast 22,369-383. 

Mitogen activated protein kinase (MARK) cascades are three kinase modules activated by phosphorylation. The three kinase modules are composed of a MAPK, a MAPKK, and a MAPKKK. There are multiple members of each component of the MAPK cascade that are conserved from yeast to human. Activation of selective MAPK modules by specific stimuli regulates cell functions such as gene expression, adhesion, migration, differ entiation, and apoptosis. 

The test system was considerably less sensitive to endosulfan when mouse ER, rather than human ER, was used to mediate (3-gal activity (Ramamoorthy et al. 1997). In similar assays, endosulfan at 10 jM had no effect on (3-gal activity in yeast Saccharomyces) transfected with either the human or rainbow trout ER (Andersen et al. 1999). In addition, no effect was observed on transcriptional activation of HeLa cells transfected with plasmids containing an estrogen receptor as a responsive element (Shelby et al. 1996). Endosulfan also did not induce transient reporter gene expression in MCF-7 human breast cancer cells at an incubation concentration of 2.5 pM (Andersen et al. 1999). Maximum endosulfan-induced ER-mediated luciferase reporter gene expression occurred in vitro in a T47D human breast adenocarcinoma cell line at approximately 10 pM, while 50% expression of luciferase occurred at about 5.9 pM the maximum expression was approximately 59% of the effect from exposure to 0.03 nM estradiol (0.00003 pM) (Legler et al. 1999). Luciferase expression from combined treatment with endosulfan and dieldrin was additive over concentrations ranging from 3 to 8 pM. 

Keaveney M, Struhl K Activator-mediated recruitment of the RNA polymerase machinery is the predominant mechanism for transcriptional activation in yeast. Mol Cell 1998 1 917. Lemon B, Tjian R Orchestrated response a symphony of transcription factors for gene control. Genes Dev 2000 l4 255l. Maniatis T, Reed R An extensive network of coupling among gene expression machines. Nature 2002 416 499. 

Herskowitz I., Rine J. Strathem J.N. (1992) Mating-type determination and mating-type interconversion in Saccharomyces cerevisiae. In The Molecular and Cellular Biology of the Yeast Saccharomyces cerevisiae. vol. 2, Gene Expression (eds E.W. Jones, J.R. Pringle J.R. Broach), pp. 583-656. Cold Spring Harbor, NY Cold Spring Harbor Laboratory. 

Friedman et al. (2000) tested this approach on a data set for yeast cell cycle expression patterns provided by Spellman and co-workers (1998), writing that This data set contains 76 gene expression measurements of the mRNA levels of 6177 S. cerevisiae ORFs. These experiments measure six... 

MacKay, V. L., Li, X., Flory, M. R., Turcott, E., and Law, G. L. (2004). Gene expression analyzed by high-resolution state array analysis and quantitative proteomics Response of yeast to mating pheromone. Mol. Cell Proteomics 3, 478—489. 

Some of the gene expression profiling analysis biochips on the market include Affymetrix s standardized GeneChip for a variety of human and yeast genes and HyX Gene Discovery Modules for genes from tissues of the cardiovascular and from tissues that are germ infected. 

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