Gene discovery

The PCR technique is very useful during all stages of the research and development of biotech crops. PCR analysis is used for gene discovery, event selection, screening, transformant identification, line selection and plant breeding. Quantitative real-time PCR is used to determine the number of transgene copies inserted in experimental... 

Andrews J et al. Gene discovery using computational and microarray analysis of transcription in the Drosophila melanoga-ster testis. Genome Res 2000 10 2030-2043. 

Some of the gene expression profiling analysis biochips on the market include Affymetrix s standardized GeneChip for a variety of human and yeast genes and HyX Gene Discovery Modules for genes from tissues of the cardiovascular and from tissues that are germ infected. 

TRETHEWY, R.N., Gene discovery via metabolic profiling, Current Opin. Biotechnol., 2001,12,135-138. 

Gene Expression Profiling Gene Discovery Pathways p53... 

Craven RJ, Lightfoot H, Cance WG. A decade of tyrosine kinases from gene discovery to therapeutics. Surg Oncol 2003 12 39-49. 

Collier LS, Carlson CM, Ravimohan S et al (2005) Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse. Nature 436 272-276... 

Copeland NG, Jenkins NA (2010) Harnessing transposons for cancer gene discovery. Nat Rev Cancer 10 696-706... 

Dubnau J, Tully T (1998) Gene discovery in Drosophila new insights for learning and memory. Annu Rev Neurosci 21 407-444... 

Mazur, B. Krebbers, E. Tingey, S. (1999) Gene discovery and product development for grain quality traits. Science, 285, 372-5. 

Polyak K, Riggins GJ. Gene discovery using the serial analysis of gene expression technique implications for cancer research. J C/i Oncol 2001 19 2948-2958. 

Foster, J.M. and Johnston, D.A. (2002) Helminth genomics from gene discovery to genome sequencing. Trends in Parasitology 1 8, 241-242. 

Merrick, J.M., Osman, A., Tsai, J., Quackenbush, J., LoVerde, P.T. and Lee, N.H. (2003) The Schistosoma mansoni gene index gene discovery and biology by reconstruction and analysis of expressed gene sequences. Journal of Parasitology 89, 261-269. 

Rabelo, E.M., Franco, G.R., Azevedo, V.A., Pena, H.B., Santos, T.M., Meira, W.S., Rodrigues, N.A., Ortega, J.M. and Pena, S.D. (1997) Update of the gene discovery program in Schistosoma mansoni with the expressed sequence tag approach. Memorias do Instituto Oswaldo Cruz 92, 625-629. 

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