Yeast gene

Janke, C., Magiera, M. M., Rathfelder, N., Taxis, C., Reber, S., Maekawa, H., Moreno-Borchart, A., Doenges, G., Schwob, E., Schiebel, E., and Knop, M. (2004). A versatile toolbox for PCR-based tagging of yeast genes New fluorescent proteins, more markers, and promoter substitution cassettes. Yeast 21, 947-962. 

Knop, M., Siegers, K., Pereira, G., Zachariae, W., Winsor, B., Nasmyth, K., and Schiebel, E. (1999). Epitope tagging of yeast genes using a PCR-based strategy More tags and improved practical routines. Yeast 15, 963—972. 

Some of the gene expression profiling analysis biochips on the market include Affymetrix s standardized GeneChip for a variety of human and yeast genes and HyX Gene Discovery Modules for genes from tissues of the cardiovascular and from tissues that are germ infected. 

Yeast gene arrays, 26 490, 491 Yeast gene deletion libraries, 26 490-492 Yeast genome, 22 515, 26 446 comparison of, 26 450t Yeast leavening... 

Thompson, M. J., et al.. Cloning and characterization of two yeast genes encoding members of the... 

Patton, E. E., Willems, A. R., Sa, D., Kuras, L, Thomas, D., Craig, K. L, and Tyers, M. Cdc53 is a scaffold protein for multiple Cdc34/Skpl/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast. Genes Dev. 1998, 32, 692-705. 

S. A. The 19S complex of the proteasome regulates nucleotide excision repair in yeast. Genes. Dev. 2001, 15, 1528-1539. 

CHDl Chdlp Not known In yeast involved in the regulation of 2-4% of yeast genes. Approximately... 

Figure 5.5 Examining regulation of yeast genes from evolving strains. (From Perea, T.L. et al., Proc. Natl. Acad. Sci. USA, 96, 9721-9726, 1999. With permission.)...

Barral, Y., Jentsch, S., and Mann, C. (1995). G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast. Genes Dev 9, 399-409. 

Semenza, J.C., Hardwick, K.G., Dean, N and Pelham, H.R. (1990). ERD2, a yeast gene reqnired for the receptor-mediated retrieval of Inminal ER proteins from the secretory pathway. Cell 61 1349-1357. 

Yeast cells can exist as haploids of opposite mating types (either a or a). When an a and an a cell are allowed to mate, they form a diploid cell (a/a). To study interactions between two proteins, cDNA sequences of a protein of interest (PT1) are expressed as a fusion protein, linked to a DNA-binding domain (DBD) of a yeast gene-transcript activator in a haploid cell (e.g., a). cDNA sequences corresponding to another test protein (PT2) are linked to the Continued on next page)... 

Figure 16.1. Schematic representation of the yeast two-hybrid system for evaluation of protein-protein interactions. Haploid yeast of a and a cells can mate to form (a/a) diploid cells. (A) If two test proteins, PT1 and PT2—expressed in (a/a) diploid cells as fusion proteins of DNA binding domains (DAB) and activation domains (AD) of yeast gene-transcript activator proteins—bind to each other, the binding interaction allows the diploid cells to grow in histidine selection media. Histidine selection media is permissive for diploid cells that express the HISS reporter gene only if PT1 and PT2 interact. (B) If PT1 and PT2 do not interact, no HISS gene product is expressed and the hybrid cell cannot grow in histidine media.

---