Volume and Mix

Figure 5-41. Scale-up relation for power, volume, and mixing slope. By permission, Rushton, J. H. and Oldshue, J. Y., Chem. Engr. Prog., V. 49, No. 4,1953, p. 161 [20].

Test 2. Transfer 40 mg to a 100 mL volumetric flask, dissolve in 50 mL of isopropyl alcohol, add 10 mL of 0.1 N hydrochloric acid, dilute with isopropyl alcohol to volume, and mix the ultraviolet absorption spectrum of this solution exhibits maxima and minima at the same wavelength as that of a similar solution of USP Miconazole RS, concomitantly measured. 

Dragendorff s reagent Dissolve 0.85 g of bismuth subnitrate in a mixture of 40 mL of water and 10 mL of glacial acetic acid (,Solution A). Dissolve 8 g of potassium iodide in 20 mL of water (Solution B). Transfer 5 mL of Solution A, 5 mL of Solution B, and 20 mL of glacial acetic acid to a 100 mL volumetric flask, dilute with water to volume, and mix. 

Standard preparation Dissolve an accurately weighed quantity of USP Miconazole RS in Mobile phase and dilute quantitatively, and stepwise if necessary, with Mobile phase to obtain a solution having a known concentration of about 0.5 mg/mL. Transfer 10 mL of this solution to 100 mL volumetric flask, dilute with Mobile phase to volume, and mix to obtain a Standard preparation having a known concentration of about 50 pg/mL. 

Assay preparation. Transfer not less than 20 Capsules to a blender jar or other container, and add about 150 mL of methylene chloride, and cool in a solid carbon dioxide acetone mixture until the contents have solidified. If necessary, transfer the mixture of capsules and methylene chloride to a blender jar, and blend with high-speed blender until all the solids are reduced to fine particles. Transfer the mixture to a 500-mL volumetric flask, add n-heptane to volume, mix, and allow solids to settle. Transfer an accurately measured volume of this solution, equivalent to 250 mg of valproic acid, to a 100 mL volumetric flask, dilute with w-heptane to volume, and mix. Transfer 5.0 mL to a container equipped with a closure. Add 2.0 mL of the internal standard solution, close the container, and mix. 

Weigh and finely powder not less than 20 methimazole tablets. Weigh accurately a portion of the powder equivalent to about 120 mg of methimazole and place in 100 ml volumetric flask. Add about 80 ml of water, insert the stopper and shake by mechanical means or occasionally by hand during 30 minutes, dilute with water to volume and mix. Filter and transfer 50.0 ml of the filtrate to a 125 ml conical flask. Add from a burett3.5 ml of 0.1N NaOH, mix, and add with agitation about 7 ml of 0.1N AgN03. Add 1 ml of bromothymol blue T.S. and continue the titration with 0.1N NaOH until a permanent, blue green color is produced. Each ml of 0.1N NaOH is equivalent to 11.42 mg of C,H,N S. 

Therefore, weigh accurately 0.5488 g of pure K2Cr207 and transfer it quantitatively into a 250 ml volumetric flask, dissolve in DW, make up the volume and mix thoroughly. 

Place 500 ml of water in a 1 L volumetric flask. Carefully measure 21 ml of cone, hydrochloric acid in a 25 ml graduated cylinder and add to the flask. Rinse the cylinder with water and add the washings to the flask. Mix, dilute to volume and mix again. Add 0.5 ml of Brij 35 and mix thoroughly. 

Add 8.4 ml of cone, hydrochloric acid to a volumetric flask containing 70 ml of water. Dilute to volume and mix. 

Add 330 g of potassium hydroxide to a 1 L volumetric flask containing 300 ml of water. Cool, dilute to volume and mix. 

Add 7.5 ml of 1 N sodium hydroxide to a volumetric flask containing 800 ml of water. Mix, dilute to volume, and mix again. 

Weigh 40 g of sodium hydroxide pellets and transfer to a pyrex 100 ml volumetric flask containing 50 ml of water. Swirl or mix to dissolve. Cool to room temperature, dilute to volume and mix again. 

Assay preparation Transfer approximately 0.24 g of sorbitol to a 50 mL volumetric flask, dissolve in a 10 mL of water, dilute with water to volume and mix. 

Add purified water to volume and mix well, for a minimum of 30 minutes. 

Assay preparation Transfer about 100 mg of Omeprazole, accurately weighed, to a 50-ml volumetric flask, dissolve in and dilute with Diluent to volume, and mix. Transfer 5 ml of this solution to a 50-ml volumetric flask, dilute with Diluent to volume, and mix. 

Implications for production include a re-allocation of volume and mix to optimize re-import opportunities from China. This will have consequences for existing plants in other locations - some may need to be closed, others upgraded or refurbished. Companies also need to consider how to transfer best practices between plants in different countries. 

Weigh and finely powder twenty tablets. Weigh accurately a portion of the powder, equivalent to about 100 mg of cimetidine and transfer to a 200 ml volumetric flask. Add 75 ml of 0.1N sulfuric acid, and shake mechanically for thirty minutes. Dilute to volume with the same solvent, mix, and filter through Whatman 1 filter paper, discarding the first 15 ml of filtrate. Pipet 5.0 ml of filtrate into a 200 volumetric flask, add 0.1N sulfuric acid to volume, and mix. Record the ultraviolet absorption spectrum of this solution frcm 300 to 215 nm in a 1 cm cell... 

System Suitability Preparation - Pipet 5 ml of the stock solution, prepared as directed under Standard preparation, into a 25-ml volumetric flask, dilute with isooctane to volume, and mix. Heat this solution at reflux for 2 hours, cool, and irradiate for 1 hour under long-wavelength and short-wavelength ultraviolet light. This solution contains cholecalciferol, pre-cholecalciferol, and tachysterol. 

This compound is determined using HPLC analysis. A Diluent reagent is prepared as a 9 1 mixture of water and methanol. The Mobile Phase is prepared as a filtered and degassed solution by dissolving 5.6 g of monobasic potassium phosphate in 820 mL of water in a 1-liter volumetric flask, adjusting with phosphoric acid to a pH of 4.3, diluting with methanol to volume, and mixing. Adjustments in the composition may be made if required by the System Suitability requirements. 

Standard Stock Solution 1 Transfer about 25 mg of benzoic acid reference standard, accurately weighed, to a 250-mL volumetric flask, add 5.0 mL of Stock Solution, dilute with Mobile Phase to volume, and mix. 

At the close of the dialysis the plasma was centrifuged to remove small amounts of precipitate and the clear plasma resulting was subjected to starch-block preparative zone electrophoresis by the technique of Kunkel and Slater (15). At the close of the electrophoresis 1-cm. segments of the starch block were cut and transferred to sintered glass funnels, and the proteins were quantitatively eluted with five successive 2-ml. aliquots of 0.9% sodium chloride. The filtrates containing the protein fractions were then made up to a constant volume and mixed, and small aliquots were analyzed for total protein content by the method of Lowry et al. (18). 

Sample Solution Transfer 1.00 0.05 g of previously dried sample, accurately weighed, into a silica or porcelain dish. Ash in a muffle furnace at 550° for 2 to 4 h. Allow the ash to cool, and dissolve in 5 mL of 20% hydrochloric acid, warming the solution if necessary to complete solution of the residue. Filter the solution through acid-washed filter paper into a 1000-mL volumetric flask. Wash the filter paper with hot water, dilute to volume, and mix. Use a 1 300 aqueous dilution as the Sample Solution. 

Standard Solutions Transfer 190.7 mg of potassium chloride, previously dried at 105° for 2 h, into a 1000-mL volumetric flask, dilute to volume with water, and mix. Transfer 100.0 mL of this solution to a second 1000-mL volumetric flask, dilute to volume with water, and mix to obtain a Stock Solution containing 10 jxg of potassium per milliliter (equivalent to 19.07 (xg of potassium chloride). Pipet 10.0-, 15.0-, and 20.0-mL aliquots of the Stock Solution into separate 100-mL volumetric flasks add 2.0 mL of a 1 5 solution of sodium chloride and 1.0 mL of hydrochloric acid to each dilute with water to volume and mix. The Standard Solutions obtained contain, respectively, 1.0, 1.5, and 2.0 (ig of potassium per milliliter. 

Standard Solutions Prepare a series of lead standard solutions serially diluted from the Standard Lead Solution. Pipet 2, 5, 10, and 20 mL, respectively, of Standard Lead Solution into separate 100-mL volumetric flasks, add 1 mL of nitric acid, dilute to volume, and mix. The Standard Solutions contain, respectively, 0.20, 0.50, 1.00 and 2.00 pig of lead per milliliter. 

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