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Urea, labelled

The first enzyme to be crystallized (and hence the first evidence that enzymes are proteins) was urease, which catalyses the hydrolysis of urea to ammonium and carbon dioxide. The original preparation of urease was from plant material, but the enzyme is also known to occur in a number of bacteria. In this study, a group of volunteers were given an intravenous infusion of 20 mmol urea labelled with both and and their urinary excretion of label was measured over 24 hours. Complete recovery of the label in urine would amount to 40 mmol and 20 mmol C. The results are shown in column 2 of Table 9.19. The experiment was repeated a week later, after they had received the antibiotic neomycin for 4 days to sterilize the gut these results are shown in column 3. [Pg.283]

The estimation. Label two 250 ml. conical flasks A and B, and into each measure 5 ml. of urine solution (or about o i g. of solid urea, accurately weighed). Add to each about 20 ml. of water and bring the temperature to about 60°. To A add 3 drops of phenolphthalein solution and to B add i ml. of 0-5% mercuric chloride solution. Now to each solution, add 10 ml. of the urease solution and mix well. The mixture A soon turns red. [Pg.520]

Biosynthesis of Protein. The dynamic equilibrium of body protein was confirmed by animal experiments using A/-labeled amino acids in 1939 (104). The human body is maintained by a continuous equilibrium between the biosynthesis of proteins and their degradative metabolism where the nitrogen lost as urea (about 85% of total excreted nitrogen) and other nitrogen compounds is about 12 g/d under ordinary conditions. The details of protein biosynthesis in living cells have been described (2,6) (see also Proteins). [Pg.282]

Synthetic chemical approaches to the preparation of carbon-14 labeled materials iavolve a number of basic building blocks prepared from barium [ CJ-carbonate (2). These are carbon [ C]-dioxide [ CJ-acetjlene [U— C]-ben2ene, where U = uniformly labeled [1- and 2- C]-sodium acetate, [ C]-methyl iodide, [ C]-methanol, sodium [ C]-cyanide, and [ CJ-urea. Many compHcated radiotracers are synthesized from these materials. Some examples are [l- C]-8,ll,14-eicosatrienoic acid [3435-80-1] inoxn. [ CJ-carbon dioxide, [ting-U— C]-phenyhsothiocyanate [77590-93-3] ftom [ " CJ-acetjlene, [7- " C]-norepinephrine [18155-53-8] from [l- " C]-acetic acid, [4- " C]-cholesterol [1976-77-8] from [ " CJ-methyl iodide, [l- " C]-glucose [4005-41-8] from sodium [ " C]-cyanide, and [2- " C]-uracil [626-07-3] [27017-27-2] from [ " C]-urea. All syntheses of the basic radioactive building blocks have been described (4). [Pg.438]

The sampling kit consists of the labelled urea, which is a drug a sampling straw, which is a device and a sample container, which would be considered an in-vitro diagnostic (IVD) medical device under EU definitions. Other examples of diagnostic drug products used in conjunction with medical devices include dyes administered to visualise blocked veins and arteries. [Pg.19]

Ammonia, Ammonium nitrate, Diphenyl carbonate Buzard, J. et al., J. Amer. Chem. Soc., 1952, 74, 2925-2926 During the synthesis of 15N-labelled urea by interaction of labelled ammonium nitrate, liquid ammonia and diphenyl carbonate in presence of copper powder, a series of explosions of the refrigerated sealed tubes was encountered. This was almost certainly caused by condensation of traces of oxygen in the tubes cooled to — 196°C dining condensation of ammonia before sealing the tubes. Cooling to —80°C would have been adequate and have avoided the hazard. [Pg.1854]

An analogous series of reactions is used to produce depyrimidinated DNA fragments. Hydrazine is used in these reactions, since both cytosine and thymine react with hydrazine. The bases are cleaved to yield urea and a pyrazole ring. The deoxyribose moiety is left as a hydrazone. Piperidine, which reacts with the hydrazone, is used to cleave the nucleotide chain. Cytosines react specifically with hydrazine in 5 mol/ L NaCl, but no specific reaction exists for thymines. Consequently, one aliquot yields labeled oligonu-cleotides 3 -terminated at cytosines, whereas a second aliquot contains nucleotides cleaved in the absence of NaCI at both cytosine and thymine residues. [Pg.247]

A competitive version of ABPP identifies the target(s) and assesses the selectivity of an enzyme inhibitor in biological systems by gauging how well the inhibitor slows the enzyme s reaction with an ABP. For example, fluorophosphonate ABP 3 was used to profile the selectivity of fatty acid amide hydrolase (FAAH) inhibitors within the serine hydrolase superfamily [27] (FAAH hydrolyzes endocannabinoids such as anandamide). Serine hydrolases that exhibited reduced labeling by the probe in the presence of inhibitor were scored as targets of the inhibitor. Urea FAAH inhibitors exemplified by PF-3845 (5) that covalently modify the active-site serine nucleophile of FAAH were found to be exquisitely selective for FAAH in brain and liver... [Pg.351]

Urea acts not only as an ammonia source but also forms decomposition products, such as biuret and higher condensation products. 14C labeling has indicated that the carbon atom of the urea molecule is not incorporated into the phthalocyanine structure. Employing a phthalic anhydride molecule bearing one radioac-tively labeled carbonyl function affords labeled copper phthalocyanine and phthalimide (as a side product), while the liberated carbon dioxide was found not to show any radioactivity. Labeled carbon dioxide, on the other hand, has been obtained in corresponding experiments using 14C labeled urea. [Pg.431]

CASRN 57-13-6 molecular formula CH4N2O FW 60.06 Biological. In a 42-d experiment, C-labeled urea applied to soil-water suspensions under aerobic and anaerobic conditions gave C02 yields of 66.3 and 70.1%, respectively (Scheunert et al, 1987). [Pg.1620]

Chemicals. Urea (99+%), glucose and D2O (99.8%) were obtained from Aldrich Chemical Co. (Gold Label). [Pg.209]

Nitrogen-15 NMR has been used to study the course of acylation and carbamidization reactions of 3-amino-5-methylthio-1,2,4-thiadiazole (3). Using a N label in the 2-position of (3), its reaction with hard nucleophiles was found to proceed via initial reactioa on the 2-position followed by a Dimroth rearrangement to give the acylated product (4) with the N label in the exocyclic position. The reaction of (3) with soft nucleophiles, such as methyl isocyanate occurs directly on the exocyclic nitrogen to give the urea (5) (Scheme 1) <84CHEC-l(6)463 >. [Pg.310]


See other pages where Urea, labelled is mentioned: [Pg.308]    [Pg.1857]    [Pg.85]    [Pg.69]    [Pg.69]    [Pg.277]    [Pg.308]    [Pg.1857]    [Pg.85]    [Pg.69]    [Pg.69]    [Pg.277]    [Pg.355]    [Pg.355]    [Pg.456]    [Pg.13]    [Pg.141]    [Pg.457]    [Pg.156]    [Pg.19]    [Pg.247]    [Pg.90]    [Pg.215]    [Pg.179]    [Pg.145]    [Pg.161]    [Pg.476]    [Pg.166]    [Pg.160]    [Pg.353]    [Pg.491]    [Pg.465]    [Pg.382]    [Pg.77]    [Pg.107]    [Pg.107]    [Pg.300]    [Pg.420]    [Pg.55]    [Pg.27]    [Pg.32]    [Pg.165]   
See also in sourсe #XX -- [ Pg.58 , Pg.61 ]

See also in sourсe #XX -- [ Pg.58 , Pg.61 ]




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