Uniform labeling

HL-60 cellular proteins were labeled uniformly with [35S]methionine and then oxidized with H202. The proteins were then assayed for the presence of Met(O) using either the CNBr assay or amino acid analysis. 

EQUIPMENT Labeling equipment clearly labeled uniform coding mislabeled or not labeled... 

In order to establish which form is present in the solution, /(15N,15N) coupling constants were measured using ID 15N INADEQUATE on a compound that was 20% 15N-labelled uniformly in all four nitrogen positions. Values of 12.1-12.3 Hz in different solvents were obtained. These correspond to the 1/(15N,15N) between Nl and N2 (N4 and N5) and prove that this compound exists exclusively in the... 

Double labeling. 3-Phosphoglycerate labeled uniformly with is incubated with 1,3-BPG labeled with 32p at C-1. What is the radioisotope distribution of the 2,3-BPG that is formed on addition of BPG mutase ... 

Nick translation widely used classical method producing probes of varying lengths and labels uniformly incorporated requires ds DNA amount of DNA decreases during labeling... 

NMR has been greatly enhanced by the ability to produce proteins labeled uniformly or at specific sites with and with the use of recom-... 

The most convincing evidence that ascorbic acid can be synthesized from a hexose sugar in animals comes from the work of Jackel and his collaborators (1950). When D-glucose labeled uniformly in all positions with was supplied to rats which had been fed chloretone to stimulate the synthesis of the vitamin, L-ascorbic acid was excreted which was also labeled uniformly. This clearly indicates that n-glucose can serve as an ultimate precursor but does not exclude the possibility that the glucose may have to be converted to other sugars before being transformed. 

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