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Untreated macrophages

Gomez-Flores et al. (2000) found that endotoxin-free methanol extracts from P. major leaves, at doses of 50, 100, 250, and 500 pg/ml, were associated with 4.4 1, 6 1, 12 0.4, and 18 0.4-fold increases of NO production, and increased TNF production (621 31, 721 36, 727 36, and 1056 52 U/ml, respectively) by peritoneal macrophages, in the absence of IFNy or LPS. NO and TNF production by untreated macrophages was negligible. In addition, P. major extracts potentiated ConA-induced lymphoproliferation (3- to 12-fold increases) in a dose-dependent fashion, compared with the effect of ConA alone. [Pg.494]

Figure 2 PBMCs were placed in a 3-pm filter above a well that contained the supernatant of untreated macrophages (left) or LPS-stimulated macrophages (right). After a 90 min incubation at 37 °C, filters were fixed and stained with hematoxylin, and migrated monocytes were counted. Figure 2 PBMCs were placed in a 3-pm filter above a well that contained the supernatant of untreated macrophages (left) or LPS-stimulated macrophages (right). After a 90 min incubation at 37 °C, filters were fixed and stained with hematoxylin, and migrated monocytes were counted.
Close membrane contacts between activated macrophages and tumor cells YC-8 were observed by electron microscopy (Puvion et al., 1975). Many tumor cells, fixed by macrophages, had cavities and probably were dead. Such a picture has never been observed with untreated macrophages. C. parvum was most effective against immunogenic tumors induced by chemical carcinogens. [Pg.239]

HIV is an RNA retrovirus that infects CD4 lymphocytes, macrophages,and dendritic cells.Untreated HIV infection causes the progressive loss of CD4 T cells, the immune system white blood cells that protect against infection and malignancy. AIDS is diagnosed based on a low CD4 count, a high viral load, and the increased susceptibility to various infections or malignancies. [Pg.204]

Similar to the in vitro study using Lewis lung cells, it was observed that animals pretreated with pyran had no detectable Ehrlich ascites cells in phase GgM and only a few in the S phase after 2 or 6 days (Table XIV) (60) compared to pyran untreated mice which had 58 in the S phase and 22 in the GgM phase. Consequently, the use of pyran activated macrophage resulted in a) a high overall level of tumoricidal activity both in vivo and in vitro, b) the appearance of a tumor cell population with 50 of their normal DNA content and c) a shift of tumor cells from phase GgM to G. These results indicate that a possible mechanism of activated macrophage tumoricidal activity involves the induction of tumor cells with a reduced DNA content. [Pg.216]

In order to measure secretion of lysosomal enzymes, macrophages of normal, untreated NMRI mice were incubated in serum-free culture medium (RPMI) and treated with different amounts of compounds (1c), (3a), (4a) (mol.wt. 16,800),and (1d), (3b), (3c) (mol.wt. 24,000). Secretion of the following enzymes was measured after 24 h of incubation using established biochemical test systems ... [Pg.91]

Currently, there is only one formulation of Amikacin in liposomes under clinical trials for treatment of tuberculosis. The antibiotic was found to be twofold to sixfold more active than the free drug in an acute experimental model of murine tuberculosis in which bacteria are located in macrophages. The count of viable bacteria found in the liver and spleen was reduced by a factor of 3-log compared with the untreated mice [158]. However, in human, this formulation was not as active as expected. To explain the disappointed results obtained, it was suggested that the liposomes could target the antibiotics in the macrophages but this was not enough to reach extracellular bacilli, which are clustered in cavity caseum in the human... [Pg.139]

FIGURE 6.1 Viability(MTT assay) of mouse peritoneal macrophages after 20 h of incubation with SLN, polystyrene particles, or control emulsion (1 1 mixture of soybean oil and MCT stabilized with Lipoid S75) of different sizes at a concentration of 0.1% (means + standard deviation, n = 3). The viability of untreated cells is 100%, and the IL-6 secretion is 1.0. D 114, Dynasan 114 L S75, Lipoid S75 CPC, cetylpyridinium chloride SO, soybean oil. [Pg.5]

FIGURE 6.9 Viability of peritoneal macrophages of the mouse after 20 h of incubation with SLN (different surfactants and Dynasan 114) in different concentrations (means + standard deviation, n = 3, untreated cells have a viability of 100%). [Pg.12]


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See also in sourсe #XX -- [ Pg.315 , Pg.315 ]




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