Umbilical vein cells, culture

A layer-by-layer microfluidics technology was used to construct a 3D microscale hierarchical tissue-like structure. For instance, three layers of tissues, fibroblasts (human lung), myocytes (smooth muscle cells), and endothelial cells (human umbilical vein) were cultured on top of each other using consecutive microchannel cell matrix delivery. Cell viability was confirmed by fluorescent staining [862]. 

Kaneko et al. (1993) have described a group of lipophilic ascorbic-acid analogues that have been studied in cultured human umbilical vein endothelial cells that were first incubated with test drug and then exposed to lipid hydroperoxides. Although ascorbate itself did not protect the endothelial cells, derivatives like CV3611 protected. Pretreatment was necessary. CV3611 was synergistic with vitamin E. The authors concluded that these lipophilic antioxidants incorporate into endothelial cell membranes where they are effective inhibitors of lipid peroxidation. In contrast, lipophobic antioxidants were not effective in their hands (Kaneko et al., 1993). 

Fig. 9.2. Physiological concentrations of estradiol decrease the production of F2a-isoprostanes in medium from cultured human umbilical vein endothelial cells in culture. (From Hermenegildo et al. 2002)...

Halichlorine (1) (Fig. 11.1) is an alkaloid which was isolated from the marine sponge H. okadai Kadota (Kuramoto et ah, 1996). This compound was revealed to be a novel alkaloid containing an azaspiro[4.5]decane skeleton clarified by detailed spectroscopic analyses. The absolute stereostructure was confirmed by many synthetic studies (Arimoto et ah, 1998 Clive et ah, 2005 Liu et ah, 2009 Trauner et ah, 1999). Halichlorine was shown to inhibit the induction of vascular cell adhesion molecule-1 (VCAM-1) in cultured human umbilical vein endothelial cells. Drugs that block the inflammatory stimuli-induced expression of VCAM-1 may be useful for treating atherosclerosis, coronary artery diseases, angina, and noncardiovascular inflammatory diseases (Kock et ah, 1995). We introduce here the recent aspects of the biological and physiological activities of halichlorine. 

Min C, Kang E, Yu SH, Shinn SH, Kim YS. Advanced glycation end products induce apoptosis and procoagulant activity in cultured human umbilical vein endothelial cells. Diabete Res Clin Pract 1999 46 197-202. 

Simak J, Holada K, Vostal JG. Release of annexin V-binding membrane microparticles from cultured human umbilical vein endothelial cells after treatment with camptothecin. BM Cell Biol 2002 3 11. 

Endothelial cells produce prostacyclins in response to ATP. AlF41 enhanced the stimulatory effect of ATP on prostacyclin release [101]. A dose-and time-dependent generation of inositol phosphates, release of arachidonic acid, and the production of prostacyclin (PGI2) in human umbilical vein endothelial cells was described [102], Stimulation of arachidonic acid release and prostacyclin production by AlF41 was described in cultured endothelial cells from rabbit coronary microvessels [103],... 

Cajero-Juarez M, Avila B, Ochoa A et al. (2002) Immortalization of bovine umbilical vein endothelial cells a model for the study of vascular endothelium. Eur J Cell Biol 81 1-8 Gerhart DZ, Broderius MA, Drewes LR (1988) cultured human and canine endothelial cells from brain microvessels. Brain Res BuU 21 785-793... 

Sharma, S., Vinh, j., Vaubourdolle, M., Baudin, B. (2003). Proteomic study of human umbilical vein endothelial cells in culture. Proteomics 3, 714—723. 

Jalfe, E. A., Nachman, R. L., Becker, C. G. and Minick, C. R. (1973). Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J. Clin. Invest. 52, 2745-2756. 

The development of parallel-plate perfusion chambers [67,68] made possible the study of platelet interaction with the extracellular matrix (ECM) generated by cells in culture or with isolated subendothelial components under defined experimental conditions. The use of the ECM produced by human umbilical vein endothelial cells (HUVEC) in culture as adhesive substrate has Su tated the understanding of the mechanisms involved in primary hemostasis [68]. HUVECs are immature and not subjected to flow conditions during their culture, two Ikctors which may influence the reactivity of their ECM towards platelets [69]. Interestingly, the properties and reactivity of the underlying ECM can be modified by exposure of HUVECs to different stimuli, an experimental approach which has fevored the investigation of basic mechanisms of thrombosis [33]. 

The generally accepted view is that ECE is a metalloprotease. Enzymes of this type have been isolated from a variety of tissue sources including cultured endothelial cells [65], vascular smooth muscle [66], human umbilical vein [67] and human brain [68], and have been characterized as neutral endopeptidases sensitive to phosphoramidon. Several groups have demonstrated an endothelial cell derived ECE which is active at neutral pH and inhibited by phosphoramidon and other metal chelators, but not by... 

Kyan-Aung, U., Haskard, D.O. and Lee, T.H. (1991). Vascular cell adhesion molecule-1 in eosinophil adhesion to cultured human umbilical vein endothelial cells in vitro. Am. J. Respir. CeU Mol. Biol. 5, 445-450. 

It has been shown that combretastatin A-4 disrupts the microtubules of human umbilical vein endothelial cells (HUVECs) in culture, thus confirming that the tubulin binding properties shown in cell-free systems are retained when the compound enters cells and that tubulin binding is a significant component of the biological activity. Also 3-fluoro- and 3-chloro derivatives retained activity in human umbilical vein endothelial cells. This kind of activity against endothelial cells is extremely important, as endothelial cells play a key role in the angiogenic process. 

Human umbilical vein endothelial cells (HUEVCs) (American Type Culture Collection, Manassas, VA). 

Fig. 2. Comparison of viability of human umbilical vein endothelial cells in culture media treated with and without ATP-vesicles before and after 6 h of anoxia...

Expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) is known to be elevated at sites of inflammation. Studies have been conducted into the effects of EGCG and TF-3 on the expression of these adhesion molecules induced by interleukin-ip (IL-lp) in cultured human umbilical vein endothelial cells (HUVECs). Both compounds significantly inhibited IL-ip-induced protein expression of VCAM and ICAM in dose-dependent manners and were associated with reduced adhesion of leukocytes to HUVECs. The m-RNA level of VCAM-1 was also inhibited by these tea polyphenolics, as was the NF-KB-dependent transcriptional activity induced by IL-lp. It is concluded that these molecules exhibit anti-inflammatory and anti-invasion properties, probably via a route involving blockage of IkB kinase. 

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