Umbilical cord blood processing

Transplant with umbilical cord blood (UCB) offers an alternative stem cell source to patients who do not have an acceptable matched related or unrelated donor. When allogeneic hematopoietic cells are obtained from UCB, the cord blood is obtained from a consenting donor in the delivery room after birth and delivery of the placenta.32 The cord blood then is processed, a sample is sent for HLA typing, and the cord blood... 

Denning-Kendall, P., Donaldson, C., Nicol, A. et al. (1996) Optimal processing of human umbilical cord blood for clinical banking, Exp. //emato/.24 1394-1401. 

Hematopoietic stem cell transplantation (HSCT) is a process that involves intravenous infusion of hematopoietic stem cells from a compatihle donor into a recipient, usually following administration of high-dose chemotherapy. Hematopoietic stem cells can be derived from the bone marrow, peripheral blood, or umbilical cord blood. The rationale for HSCT in the treatment of malignant disease is based on studies that show that most anticancer drugs have a steep... 

Cadmium in umbilical cord blood and placental tissue was determined for 31 samples collected at the Gynecology Maternity Ward in Kikinda Hospital, and 30 samples collected at the Gynecology Maternity Clinic in Novi Sad, in the period 1996-1997. The samples of hepatizated blood and placental tissue were broken down by nitrous acid using a wet process, the fats were extracted using diethyl ether, and the cadmium concentration was determined by atomic absorption spectrophotometry on a graphite furnace (Vather, 1982 Stoeppler, 1983 Beauty, 1988). 

Stem Cell Transplant. Stem cells can be found in either the bone marrow or the peripheral circulating blood. They also can be found in umbilical cord blood and fetal marrow and liver. Before stem cells can be harvested from a donor, mobilization of stem cells into the peripheral blood is stimulated by the use of cytokines. A process called apheresis is used to collect the stem cells from the donor s blood. These harvested stem cells are then injected into the patient. As with a bone marrow transplant, it is also possible to perform an autologous stem cell transplant. The transplanted patient will usually begin to produce new blood cells ten to twenty-one days after receiving the harvested stem cells. 

Exposure to heavy metals especially during the gestational period may impair development of visual processing, as suggested in a follow-up study in school-age children (mean age = 10.9years). Mercury and lead concentrations in umbilical cord blood (infants) and in blood (children) were negatively correlated with pattern-reversal visual evoked potentials in children that were scalp-recorded at the occipital cortex [190 j. 

Untraumatized, freshly excised vessels are placed in cold HBSS and processed in the next few hours. Small arteries branching out from aorta have to be closed by clamping or suturing. Umbilical cord ends that have been in contact with clamps have to be cut away to ensure sterility. The vessel is flushed with PBS (100-200 ml use blunt needles to cannulate umbilical vein) until no traces of blood are visible, then its lower end is clamped and the vessel is filled with collagenase (0.5-1.0 mg/ml) in PBS prewarmed at 37°C. The upper end is then clamped, and the vessel segment is incubated for 15 min at 37° C or 40 min at room temperature. After incubation, the vessel content is poured out and the vessel is refilled a few times with 50% of its volume capacity with complete culture medium, clamped again at both ends, and agitated back and forth several... 

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