TYansforming Effects on Cells in Culture

2 Systems to Study Neoplastic Tkinsformation of Cells in Culture 

Neoplastic transformation of fibroblastic cells has been reported with Chinese hamster lung cells, rat embryo cells, BHK cells, mouse 3T3 cell lines, a mouse ventral prostate cell line, and the mouse C3H/10T1/2 cell line (Heidelberger et al, 1983 Barrett and Ts o, 1978). The Syrian hamster embryo cells and the various mouse cell lines have been employed most often in studies employing fibroblastic cells. Neoplastic and preneoplastic transformation of normal human fibroblasts has also been reported in recent years (DeMars and Jackson, 1977, Freedman and Shin, 1977 Kakunagti, 1978 Milo and DiPaolo, 1978 Namba et al, 1978 Borek, 1980 McCormick et al, 1980 Sutherland et al, 1980 SilinskasetoiL, 1981). 

A number of experimaital systems utilizing cells from a variety of species and tissues have been employed for studies of cell transformation by chemical carcinogens (Thbles 7.5 and 7.6). Because fibroblasts were employed in the initial systems, these cells are the best character- 

mammary gland (Richards et al, 1980) salivary gland (Wigley, 

and trachea (Marchok etal, 1977 Marchok etal, 1978 Steele et al, 1979 Pai et al, 1983 Thomassen et al, 1983). Neoplastic transformation of glial cells has also been reported (Laerum and Rajewsky, 1975 Roscoe and Claisse, 1978). 

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